A new high-performance, high-speed microplate reader has received HTRF (homogenous time-resolved fluorescence) accreditation that certifies that it complies with standards for detection set by a major manufacturer of HTRF reagents.

HTRF involves several carefully selected fluorophores. Fluorescence resonance energy transfer (FRET) partners must fulfill multiple compatibility criteria. First, their emission spectra must show non-overlapping regions in order to be able to measure each partner's fluorescence individually. Second, the FRET quantum yield (i.e., its efficacy) must be as high as possible. Third, fluorescence emission must occur within a region of the spectrum remote from that naturally produced by proteins; in other words, a red-shifted emission is theoretically better in order to avoid medium-intrinsic fluorescence. HTRF uses a combination of several fluorophores forming different FRET systems. HTRF's central element, the energy donor, consists of a rare earth complex in which the lanthanide ion (europium or terbium) is tightly embedded in a macrocycle. This very unique type of structure gives HTRF donors their long-lived fluorescence properties, as well as robustness that enables these molecules to be used in most assay conditions.

In order to broaden HTRF readout capabilities for use in the field of life sciences, Cisbio Bioassays (Codolet, France) has initiated partnerships with several detection system manufacturers. This collaboration has led to high standard reader certification, indicated by an HTRF sticker. This label guarantees that the reader meets the physical specifications for optimal HTRF readout.

Cisbio recently added the BioTek (Winooski, VT, USA) Synergy Neo2 Multi-Mode Reader to the list of instruments with HTRF certification.

The Synergy Neo2 Multi-Mode Reader was designed for speed and ultra-high quality performance. It incorporates BioTek's patented Hybrid technology, which combines independent monochromator-based and filter-based optics in the same instrument. Thus, continuously variable bandwidth quadruple monochromators, sensitive high transmission filter-based optics, and up to four photomultiplier tubes provide ultra-fast measurements with high quality results.

Complementing its sophisticated optic systems, the Synergy Neo2 boasts advanced environment controls, including CO2/O2 control and incubation up to 65 degrees Celsius. Variable shaking supports live cell assays, while cell-based detection is optimized with direct bottom illumination.

The instrument package is powered by BioTek's Gen5 Data Analysis Software and has been configured to integrate seamlessly with the optional BioStackNeo plate stacker, which was designed specifically for use with the Synergy Neo2.

Gary Barush, director of marketing and sales at BioTek, said, “For years BioTek has led the evolution of multimode detection. We are proud to introduce this top-of-the-line reader which combines best in class performance in all modes with the speed that customers demand. In addition to being optimized for biochemical assay performance, Synergy Neo2 provides unsurpassed results for cell based assay detection, while its modular configuration makes this new level of performance accessible for many budgets."

Related Links:
Cisbio Bioassays
BioTek