Biomarker FR+CTC Explored for Breast Cancer Diagnosis
|
By LabMedica International staff writers Posted on 30 Dec 2021 |
|
Image: A colored scanning electron micrograph (SEM) of a breast cancer cell (Photo courtesy of Case Western Reserve University School)
The prognosis of breast cancer is dramatically improved owing to progress on the early diagnosis and effective therapy, and the survivor number is higher than any other types of cancers. However, the number of new breast cancer cases reached 2.26 million in 2020, accounted for 11.7% of all new tumor cases.
Folate receptor (FR) is a glycosylated phosphatidylinositol-coupled protein with high affinity and endocytosis and transport capacity for its natural ligand folic acid. It is a cell surface protein that has been proven to be an ideal tumor marker for non-small cell lung cancer. The clinical significance of folate receptor-positive circulating tumor cells (FR+CTC) has been explored.
Clinical Laboratorians at the Renmin Hospital of Wuhan University (Wuhan, China) enrolled in a study 60 breast cancer female patients with a mean age of 52 years (range, 27–75 years) and 32 healthy controls with an age of 52 (range, 40–66 years), from January 2019 to January 2021. The diagnosis for the patients was confirmed by cytology or histopathology. Peripheral blood was collected for isolation of circulating tumor cells (CTC) on the diagnosis in all patients of the cohort. The 27 patients underwent radical mastectomy, and the peripheral blood was also collected in two weeks after the operation.
Circulating tumor cells was isolated with folate receptor-positive (FR+) CTC isolation method using CytoploRare kit (GenoSaber Biotech Co., Ltd., Shanghai, China). The method includes negative enrichment of FR+ circulating tumor cells with immunomagnetic beads to deplete leukocytes first, then CTC level was quantitated by ligand-targeted PCR. First, leukocytes and mononuclear macrophages were removed by anti-CD45 and anti-CD14 magnetic beads. Then, the cells were activated and washed to free up the folate binding site. The carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), and cancer antigen 153 (CA153) were measured by chemiluminescent immunoassay.
The investigators reported that the levels of FR+CTC were significantly higher in breast cancer patients compared with healthy controls. The levels of FR+CTC in breast cancer patients and healthy people were 10.95 (7.28–14.15) FU/3 mL and 6.93 (5.46–8.19) FU/3 mL, respectively. The detection rate of FR+CTC was decreased in 19 of 27 patients underwent the surgery in two weeks post-operation compared with pre-operation; statistical analysis showed the difference was significant. They also found that the combination of FR+CTC, CEA, CA125, and CA153 can significantly improve the diagnostic efficiency for breast cancer.
The authors concluded that the detection of FR+CTCs in peripheral blood is simple, noninvasive methods with high sensitivity and specificity. It can be used as a potential biomarker for auxiliary diagnosis and early detection in breast cancer patients. The study was published on December 17, 2021 in the Journal of Clinical Laboratory Analysis.
Related Links:
Renmin Hospital of Wuhan University
GenoSaber Biotech Co., Ltd.
Folate receptor (FR) is a glycosylated phosphatidylinositol-coupled protein with high affinity and endocytosis and transport capacity for its natural ligand folic acid. It is a cell surface protein that has been proven to be an ideal tumor marker for non-small cell lung cancer. The clinical significance of folate receptor-positive circulating tumor cells (FR+CTC) has been explored.
Clinical Laboratorians at the Renmin Hospital of Wuhan University (Wuhan, China) enrolled in a study 60 breast cancer female patients with a mean age of 52 years (range, 27–75 years) and 32 healthy controls with an age of 52 (range, 40–66 years), from January 2019 to January 2021. The diagnosis for the patients was confirmed by cytology or histopathology. Peripheral blood was collected for isolation of circulating tumor cells (CTC) on the diagnosis in all patients of the cohort. The 27 patients underwent radical mastectomy, and the peripheral blood was also collected in two weeks after the operation.
Circulating tumor cells was isolated with folate receptor-positive (FR+) CTC isolation method using CytoploRare kit (GenoSaber Biotech Co., Ltd., Shanghai, China). The method includes negative enrichment of FR+ circulating tumor cells with immunomagnetic beads to deplete leukocytes first, then CTC level was quantitated by ligand-targeted PCR. First, leukocytes and mononuclear macrophages were removed by anti-CD45 and anti-CD14 magnetic beads. Then, the cells were activated and washed to free up the folate binding site. The carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), and cancer antigen 153 (CA153) were measured by chemiluminescent immunoassay.
The investigators reported that the levels of FR+CTC were significantly higher in breast cancer patients compared with healthy controls. The levels of FR+CTC in breast cancer patients and healthy people were 10.95 (7.28–14.15) FU/3 mL and 6.93 (5.46–8.19) FU/3 mL, respectively. The detection rate of FR+CTC was decreased in 19 of 27 patients underwent the surgery in two weeks post-operation compared with pre-operation; statistical analysis showed the difference was significant. They also found that the combination of FR+CTC, CEA, CA125, and CA153 can significantly improve the diagnostic efficiency for breast cancer.
The authors concluded that the detection of FR+CTCs in peripheral blood is simple, noninvasive methods with high sensitivity and specificity. It can be used as a potential biomarker for auxiliary diagnosis and early detection in breast cancer patients. The study was published on December 17, 2021 in the Journal of Clinical Laboratory Analysis.
Related Links:
Renmin Hospital of Wuhan University
GenoSaber Biotech Co., Ltd.
New
Gold Member
Rotavirus Test
Rotavirus Test - 30003 – 30073
New
Respiratory Bacterial Panel
Real Respiratory Bacterial Panel 2
New
H.pylori Test
Humasis H.pylori Card
Latest Pathology News
- Advanced Imaging Reveals Mechanisms Causing Autoimmune Disease
- AI Model Effectively Predicts Patient Outcomes in Common Lung Cancer Type
- AI Model Predicts Patient Response to Bladder Cancer Treatment
- New Laser-Based Method to Accelerate Cancer Diagnosis
- New AI Model Predicts Gene Variants’ Effects on Specific Diseases
- Powerful AI Tool Diagnoses Coeliac Disease from Biopsy Images with Over 97% Accuracy
- Pre-Analytical Conditions Influence Cell-Free MicroRNA Stability in Blood Plasma Samples
- 3D Cell Culture System Could Revolutionize Cancer Diagnostics
- Painless Technique Measures Glucose Concentrations in Solution and Tissue Via Sound Waves
- Skin-Based Test to Improve Diagnosis of Rare, Debilitating Neurodegenerative Disease
- Serum Uromodulin Could Indicate Acute Kidney Injury in COVID-19 Patients
- AI Model Reveals True Biological Age From Five Drops of Blood
- First-Of-Its-Kind AI Tool Visualizes Cell’s ‘Social Network’ To Treat Cancer
- New Test Diagnoses High-Risk Childhood Brain Tumors
- Informatics Solution Elevates Laboratory Efficiency and Patient Care
- Microfluidic Device Assesses Stickiness of Tumor Cells to Predict Cancer Spread
Channels
Clinical Chemistry
view channel
Carbon Nanotubes Help Build Highly Accurate Sensors for Continuous Health Monitoring
Current sensors can measure various health indicators, such as blood glucose levels, in the body. However, there is a need to develop more accurate and sensitive sensor materials that can detect lower... Read more
Paper-Based Device Boosts HIV Test Accuracy from Dried Blood Samples
In regions where access to clinics for routine blood tests presents financial and logistical obstacles, HIV patients are increasingly able to collect and send a drop of blood using paper-based devices... Read more
Molecular Diagnostics
view channel
RNA-Based Blood Test Detects Preeclampsia Risk Months Before Symptoms
Preeclampsia remains a major cause of maternal morbidity and mortality, as well as preterm births. Despite current guidelines that aim to identify pregnant women at increased risk of preeclampsia using... Read more
First Of Its Kind Test Uses microRNAs to Predict Toxicity from Cancer Therapy
Many men with early-stage prostate cancer receive stereotactic body radiotherapy (SBRT), a highly precise form of radiation treatment that is completed in just five sessions. Compared to traditional radiation,... Read more
Novel Cell-Based Assay Provides Sensitive and Specific Autoantibody Detection in Demyelination
Anti-myelin-associated glycoprotein (MAG) antibodies serve as markers for an autoimmune demyelinating disorder that affects the peripheral nervous system, leading to sensory impairment. Anti-MAG-IgM antibodies... Read more
Hematology
view channel
New Scoring System Predicts Risk of Developing Cancer from Common Blood Disorder
Clonal cytopenia of undetermined significance (CCUS) is a blood disorder commonly found in older adults, characterized by mutations in blood cells and a low blood count, but without any obvious cause or... Read more
Non-Invasive Prenatal Test for Fetal RhD Status Demonstrates 100% Accuracy
In the United States, approximately 15% of pregnant individuals are RhD-negative. However, in about 40% of these cases, the fetus is also RhD-negative, making the administration of RhoGAM unnecessary.... Read more
Immunology
view channel
Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer
Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more
Machine Learning-Enabled Blood Test Predicts Immunotherapy Response in Lymphoma Patients
Chimeric antigen receptor (CAR) T-cell therapy has emerged as one of the most promising recent developments in the treatment of blood cancers. However, over half of non-Hodgkin lymphoma (NHL) patients... Read more
Microbiology
view channel
Handheld Device Deliver Low-Cost TB Results in Less Than One Hour
Tuberculosis (TB) remains the deadliest infectious disease globally, affecting an estimated 10 million people annually. In 2021, about 4.2 million TB cases went undiagnosed or unreported, mainly due to... Read more
New AI-Based Method Improves Diagnosis of Drug-Resistant Infections
Drug-resistant infections, particularly those caused by deadly bacteria like tuberculosis and staphylococcus, are rapidly emerging as a global health emergency. These infections are more difficult to treat,... Read more
Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours
Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Technology
view channel
Pain-On-A-Chip Microfluidic Device Determines Types of Chronic Pain from Blood Samples
Chronic pain is a widespread condition that remains difficult to manage, and existing clinical methods for its treatment rely largely on self-reporting, which can be subjective and especially problematic... Read more
Innovative, Label-Free Ratiometric Fluorosensor Enables More Sensitive Viral RNA Detection
Viruses present a major global health risk, as demonstrated by recent pandemics, making early detection and identification essential for preventing new outbreaks. While traditional detection methods are... Read more
Industry
view channel
Cepheid and Oxford Nanopore Technologies Partner on Advancing Automated Sequencing-Based Solutions
Cepheid (Sunnyvale, CA, USA), a leading molecular diagnostics company, and Oxford Nanopore Technologies (Oxford, UK), the company behind a new generation of sequencing-based molecular analysis technologies,... Read more
Grifols and Tecan’s IBL Collaborate on Advanced Biomarker Panels
Grifols (Barcelona, Spain), one of the world’s leading producers of plasma-derived medicines and innovative diagnostic solutions, is expanding its offer in clinical diagnostics through a strategic partnership... Read more
