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Molecular Diagnostics

Image: Illustration of a 2019- novel coronavirus (nCoV) virion (Photo courtesy of Alissa Eckert, MS/CDC).

CDC Provides Test Protocol for Novel Coronavirus

 The US Centers for Disease Control and Prevention (Atlanta GA, USA) has publicly posted its assay for novel coronavirus 2019-nCoV and is now developing the test into kits. The CDC diagnostic test is a reverse transcriptase real-time PCR (rRT-PCR) assay developed for respiratory and blood serum samples. The protocol to make the assay has been made publicly available. More...
11 Feb 2020
Image: Workflow of cancer personalized profiling by deep sequencing (CAPP-Seq) circulating tumor-DNA analysis (ctDNA) (Photo courtesy of Rashedul Islam Rony, PhD).

Lung Cancer Liquid Biopsy Guides MRD-Directed Adjuvant Therapy

 Minimal residual disease (MRD) is the name given to small numbers of leukemic cells (cancer cells from the bone marrow) that remain in the person during treatment or after treatment when the patient is in remission or have no symptoms or signs of disease. More...
11 Feb 2020
Image: Human sperm stained for semen quality testing in the clinical laboratory (Photo courtesy of Wikimedia Commons)

Detection of Pathogenic Microorganisms in Semen by Sequencing Sperm RNA

 Advanced sequencing of sperm RNA was used to detect genetic material from microorganisms that were present as contaminants in the associated semen. More...
11 Feb 2020
Image: Summary of findings in primary BRCA2mut/+ breast tissues: Epithelial progenitor cells of heterozygous germline BRCA2 carriers exhibit DNA damage, failed replication stress, and damage responses, together with attenuated apoptosis. Loss of heterozygosity (LOH) analyses suggests that these findings may reflect a haploinsufficient phenotype for BRCA2 in vivo (Photo courtesy of Massachusetts General Hospital).

Single-Cell Sequencing Identifies Breast Cancer Risk

 Women harboring heterozygous germline mutations of BRCA2 have a 50% to 80% risk of developing breast cancer, yet the pathogenesis of these cancers is poorly understood. Breast cancers arising in women who inherit heterozygous mutations in BRCA2 are associated with a high prevalence of genomic alterations and aggressive clinical behavior. More...
10 Feb 2020
Image: The flexible NextSeq 550 System offers a seamless transition between high-throughput sequencing and array scanning (Photo courtesy of Illumina).

Novel Enrichment Technology Indicates High Performance for Multi-Cancer Detection

 Breast, lung, pancreatic, and prostate cancer make up 41% of the cancer incidence in the USA. Early detection and a deep understanding of each cancer remain critical for implementing the highest quality of care. More...
10 Feb 2020
Image: Photomicrograph showing poor to moderately differentiated adenocarcinoma of the stomach (Photo courtesy of Wikimedia Commons)

Liquid Biopsy Predicts Prognosis of Gastric Cancer Following Chemotherapy or Surgery

 A liquid biopsy approach, which utilized analyses of matched circulating DNA (cfDNA) and white blood cells from the same patient, was used to predict recurrence of gastric cancer in patients after surgery. More...
10 Feb 2020
Image: The myTAIHEART test is a powerful non-invasive test that uses a small sample of blood to determine if a patient is at a low or increased risk of rejecting their heart transplant. myTAIHEART works by measuring the fraction of cell-free DNA (cfDNA) present in the bloodstream that is attributable to the transplanted heart. An elevated donor fraction (DF%) is associated with an increased risk of acute cellular rejection (Photo courtesy of TAI Diagnostics).

Heart Transplant Monitoring Test Validated

 A heart transplant, or a cardiac transplant, is a surgical transplant procedure performed on patients with end-stage heart failure or severe coronary artery disease. Noninvasive risk assessment for rejection in heart transplant recipients, both adult and pediatric, is an imperative and urgent clinical need. More...
06 Feb 2020
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