Stem Cell-Generated Stomach Organoids to Boost Gastric Disease Research
|
By LabMedica International staff writers Posted on 24 Jan 2017 |
|
Image: A confocal microscopic image showing tissue-engineered human stomach tissues from the corpus/fundus region, which produces acid and digestive enzymes (Photo courtesy of Cincinnati Children\'s Hospital Medical Center).
Research on gastric diseases will benefit from the development of complex organoid structures containing functional stomach fundic epithelium tissue that were generated from human pluripotent stem cells.
Despite the global prevalence of gastric disease, there are few adequate models in which to study the fundus epithelium of the human stomach. To fill this gap, investigators at Cincinnati Children's Hospital Medical Center differentiated human pluripotent stem cells (hPSCs) into gastric organoids containing fundic epithelium by first identifying and then recapitulating key events in embryonic fundus development.
The investigators reported in the January 4, 2017, online edition of the journal Nature that disruption of Wnt/beta-catenin signaling in mouse embryos led to conversion of fundic to antral epithelium, and that beta-catenin activation in hPSC-derived foregut progenitors promoted the development of human fundic-type gastric organoids (hFGOs). The investigators then used hFGOs to identify temporally distinct roles for multiple signaling pathways in epithelial morphogenesis and differentiation of fundic cell types, including chief cells and functional parietal cells.
"Now that we can grow both antral- and corpus/fundic-type human gastric mini-organs, it is possible to study how these human gastric tissues interact physiologically, respond differently to infection, injury and react to pharmacologic treatments," said senior author Dr. James M. Wells, director of the pluripotent stem cell facility at Cincinnati Children's Hospital Medical Center. "Diseases of the stomach impact millions of people in the United States and gastric cancer is the third leading cause of cancer-related deaths worldwide."
Despite the global prevalence of gastric disease, there are few adequate models in which to study the fundus epithelium of the human stomach. To fill this gap, investigators at Cincinnati Children's Hospital Medical Center differentiated human pluripotent stem cells (hPSCs) into gastric organoids containing fundic epithelium by first identifying and then recapitulating key events in embryonic fundus development.
The investigators reported in the January 4, 2017, online edition of the journal Nature that disruption of Wnt/beta-catenin signaling in mouse embryos led to conversion of fundic to antral epithelium, and that beta-catenin activation in hPSC-derived foregut progenitors promoted the development of human fundic-type gastric organoids (hFGOs). The investigators then used hFGOs to identify temporally distinct roles for multiple signaling pathways in epithelial morphogenesis and differentiation of fundic cell types, including chief cells and functional parietal cells.
"Now that we can grow both antral- and corpus/fundic-type human gastric mini-organs, it is possible to study how these human gastric tissues interact physiologically, respond differently to infection, injury and react to pharmacologic treatments," said senior author Dr. James M. Wells, director of the pluripotent stem cell facility at Cincinnati Children's Hospital Medical Center. "Diseases of the stomach impact millions of people in the United States and gastric cancer is the third leading cause of cancer-related deaths worldwide."
Gold Member
Immunochromatographic Assay
CRYPTO Cassette
Sample Transportation System
Tempus1800 Necto
HBV DNA Test
GENERIC HBV VIRAL LOAD VER 2.0
Latest BioResearch News
- Genome Analysis Predicts Likelihood of Neurodisability in Oxygen-Deprived Newborns
- Gene Panel Predicts Disease Progession for Patients with B-cell Lymphoma
- New Method Simplifies Preparation of Tumor Genomic DNA Libraries
- New Tool Developed for Diagnosis of Chronic HBV Infection
- Panel of Genetic Loci Accurately Predicts Risk of Developing Gout
- Disrupted TGFB Signaling Linked to Increased Cancer-Related Bacteria
- Gene Fusion Protein Proposed as Prostate Cancer Biomarker
- NIV Test to Diagnose and Monitor Vascular Complications in Diabetes
- Semen Exosome MicroRNA Proves Biomarker for Prostate Cancer
- Genetic Loci Link Plasma Lipid Levels to CVD Risk
- Newly Identified Gene Network Aids in Early Diagnosis of Autism Spectrum Disorder
- Link Confirmed between Living in Poverty and Developing Diseases
- Genomic Study Identifies Kidney Disease Loci in Type I Diabetes Patients
- Liquid Biopsy More Effective for Analyzing Tumor Drug Resistance Mutations
- New Liquid Biopsy Assay Reveals Host-Pathogen Interactions
- Method Developed for Enriching Trophoblast Population in Samples
Channels
Clinical Chemistry
view channel
VOCs Show Promise for Early Multi-Cancer Detection
Early cancer detection is critical to improving survival rates, but most current screening methods focus on individual cancer types and often involve invasive procedures. This makes it difficult to identify... Read more
Portable Raman Spectroscopy Offers Cost-Effective Kidney Disease Diagnosis at POC
Kidney disease is typically diagnosed through blood or urine tests, often when patients present with symptoms such as blood in urine, shortness of breath, or weight loss. While these tests are common,... Read more
Molecular Diagnostics
view channel
Simple Blood Test Could Reveal Kidney Disease Earlier
Kidney disease remains one of the leading causes of premature mortality, affecting 13% of the global population and nearly one-third of intensive care patients who develop acute kidney injury (AKI).... Read more
Revolutionary Blood Test Accurately Diagnoses Chronic Fatigue Syndrome
Myalgic Encephalomyelitis, also known as Chronic Fatigue Syndrome (ME/CFS), is a long-term debilitating illness that affects millions worldwide, including over 400,000 people in the UK. The condition causes... Read more
Hematology
view channel
Viscoelastic Testing Could Improve Treatment of Maternal Hemorrhage
Postpartum hemorrhage, severe bleeding after childbirth, remains one of the leading causes of maternal mortality worldwide, yet many of these deaths are preventable. Standard care can be hindered by delays... Read more
Pioneering Model Measures Radiation Exposure in Blood for Precise Cancer Treatments
Scientists have long focused on protecting organs near tumors during radiotherapy, but blood — a vital, circulating tissue — has largely been excluded from dose calculations. Each blood cell passing through... Read more
Platelets Could Improve Early and Minimally Invasive Detection of Cancer
Platelets are widely recognized for their role in blood clotting and scab formation, but they also play a crucial role in immune defense by detecting pathogens and recruiting immune cells.... Read more
Portable and Disposable Device Obtains Platelet-Rich Plasma Without Complex Equipment
Platelet-rich plasma (PRP) plays a crucial role in regenerative medicine due to its ability to accelerate healing and repair tissue. However, obtaining PRP traditionally requires expensive centrifugation... Read moreImmunology
view channel
Blood Test Tracks Treatment Resistance in High-Grade Serous Ovarian Cancer
High-grade serous ovarian cancer (HGSOC) is often diagnosed at an advanced stage because it spreads microscopically throughout the abdomen, and although initial surgery and chemotherapy can work, most... Read more
Luminescent Probe Measures Immune Cell Activity in Real Time
The human immune system plays a vital role in defending against disease, but its activity must be precisely monitored to ensure effective treatment in cancer therapy, autoimmune disorders, and organ transplants.... Read more
Blood-Based Immune Cell Signatures Could Guide Treatment Decisions for Critically Ill Patients
When a patient enters the emergency department in critical condition, clinicians must rapidly decide whether the patient has an infection, whether it is bacterial or viral, and whether immediate treatment... Read more
Microbiology
view channel
Fast Noninvasive Bedside Test Uses Sugar Fingerprint to Detect Fungal Infections
Candida bloodstream infections are a growing global health threat, causing an estimated 6 million cases and 3.8 million deaths annually. Hospitals are particularly vulnerable, as weakened patients after... Read more
Rapid Sepsis Diagnostic Device to Enable Personalized Critical Care for ICU Patients
Sepsis is a life-threatening condition that occurs when the body’s response to infection spirals out of control, damaging organs and leading to critical illness. Patients often arrive at intensive care... Read more
Pathology
view channel
Serially Testing Brain Tumor Samples Reveals Treatment Response in Glioblastoma Patients
Glioblastoma (GBM) is the most aggressive form of brain cancer, known for rapid growth, recurrence, and resistance to treatment. Understanding how tumors respond to therapy remains challenging since imaging... Read more
High-Accuracy Tumor Detection Method Offers Real-Time Surgical Guidance
Pancreatic neuroendocrine neoplasms (PNENs) are rare cancers that affect hormone-producing cells in the pancreas. Although uncommon, their incidence has been increasing, and surgery remains the only curative option.... Read more
Technology
view channel
AI Algorithm Assesses Progressive Decline in Kidney Function
Chronic kidney disease (CKD) affects more than 700 million people worldwide and remains a major global health challenge. The condition often progresses silently, and many patients remain undiagnosed until... Read more
Taste-Based Influenza Test Could Replace Nasal Swabs with Chewing Gum
Influenza is one of the most dangerous infectious diseases worldwide, claiming around half a million lives each year. What makes it particularly insidious is that flu viruses are contagious even before... Read more
3D Micro-Printed Sensors to Advance On-Chip Biosensing for Early Disease Detection
Early-stage disease diagnosis depends on the ability to detect biomarkers with exceptional sensitivity and precision. However, traditional biosensing technologies struggle with achieving this at the micro-scale,... Read more
Industry
view channel
Terumo BCT and Hemex Health Collaborate to Improve Access to Testing for Hemoglobin Disorders
Millions of people worldwide living with sickle cell disease and other hemoglobin disorders experience delayed diagnosis and limited access to effective care, particularly in regions where testing is scarce.... Read more
Revvity and Sanofi Collaborate on Program to Revolutionize Early Detection of Type 1 Diabetes
Type 1 diabetes (T1D) is a lifelong autoimmune condition in which the immune system destroys the pancreas’s insulin-producing beta cells, leading to dependence on insulin therapy. Early detection is critical... Read more
GSI Group Acquires Blood Processing Equipment Manufacturer GenesisBPS
Blood processing and storage are vital to healthcare and clinical practice, ensuring safe transfusions and cellular therapies. However, hospitals and laboratories worldwide face challenges in maintaining... Read more