We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

LabMedica

Download Mobile App
Recent News Expo Clinical Chem. Molecular Diagnostics Hematology Immunology Microbiology Pathology Technology Industry Focus

Glycated Albumin Is a Promising Marker for Diabetes

By LabMedica International staff writers
Posted on 30 Nov 2015
Print article
The Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer
The Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Photo courtesy of Thermo Fisher Scientific)
Although glucose level is the contributing factor and diagnostic marker for type 2 diabetes, there is an imperative need to look “beyond glucose” and to seek additional biomarkers that could better predict the risk for diabetes as well its diagnosis with accurate precision.

Human serum albumin is one of the most abundant plasma proteins that readily undergoes glycation, thus glycated albumin has been suggested as an additional marker for monitoring glycemic status in people with type 2 diabetes.

Scientists at the National Chemical Laboratory (Pune, India) collected blood samples from a diabetes specialized clinic as well as healthy controls. Diagnostic parameters, including fasting blood glucose, HbA1c, oral glucose tolerance test, postprandial blood sugar, lipids, urea, creatinine, and microalbumin were measured. Equal volumes of two plasma samples with similar HbA1c (deviation of less than 0.2%) were pooled, and three such pooled plasma in each group were used for mass spectrometric technical triplicate analysis.

The team used high resolution accurate mass spectrometry (HR/AM) which has paved the way for the fragment ion library that was used for quantification of glycated peptides of albumin in the context of diabetes. Glycation is a non-enzymatic chemical reaction between glucose and proteins leading to formation of advanced glycation end products (AGEs), which have been implicated in the pathogenesis of type 2 diabetes and its complications. All samples were analyzed on hybrid quadruple Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific; Waltham, MA, USA).

Muthuswamy Balasubramanyam, PhD, senior author of the study said, “While earlier studies quantified only Amadori-modified lysine (AML) modifications, our study in addition characterized the carboxymethyl-lysine (CML) and carboxyethyl-lysine (CEL) modified peptides of albumin and this is an important advancement as CML and CEL are the predominant AGEs, constituting up to 80% of total AGEs. The association of CML modified peptides of albumin with prediabetes, diabetes, and microalbuminuria is a clinically relevant and therapeutically important finding.” The study was published originally online on August 1, 2015, in the journal Molecular Cell Proteomics.

Related Links:

National Chemical Laboratory 
Thermo Scientific 


Gold Member
Flocked Fiber Swabs
Puritan® Patented HydraFlock®
Antipsychotic TDM Assays
Saladax Antipsychotic Assays
New
Silver Member
Rubella Infection ELISA
ReQuest RUBELLA IgM ELISA Kit
New
Progesterone Serum Assay
Progesterone ELISA Kit
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Molecular Diagnostics

view channel
Image: The FDA clearance for the QIAstat-Dx Respiratory Panel Mini test follows the recent approval of QIAstat-Dx Respiratory Panel Plus (Photo courtesy of QIAGEN)

Respiratory Panel to Help Clinicians Make Precise Treatment Decisions in Outpatient Settings

Respiratory tract infections are the primary reason for visits to emergency departments and subsequent hospitalizations. In the U.S., it is estimated that there are up to 41 million cases of influenza... Read more

Hematology

view channel
Image: QScout CBC will give a complete blood count in 2 minutes from fingerstick or venous blood (Photo courtesy of Ad Astra Diagnostics)

Next Gen CBC and Sepsis Diagnostic System Targets Faster, Earlier, Easier Results

Every hour is critical in protecting patients from infections, yet there are currently limited tools to assist in early diagnosis before patients reach a hospital. The complete blood count (CBC) is a common... Read more

Microbiology

view channel
Image: The InfectoSynovia test has the potential to revolutionize the diagnosis of periprosthetic joint infection (Photo courtesy of 123RF)

High-Accuracy Bedside Test to Diagnose Periprosthetic Joint Infection in Five Minutes

Periprosthetic joint infection (PJI) represents a significant global issue that is worsening as the number of joint replacements increases due to aging populations. In the United States alone, the anticipated... Read more

Pathology

view channel
Image: The new technique allows properties of cancer cells and their surrounding tissue to be analyzed in detail at single-cell level (Photo courtesy of Universität Helsinki/Karolina Punovuori)

New Imaging Method Opens Door to Precision Diagnostics for Head and Neck Cancers

Head and neck cancers, while considered rare, represent a significant portion of cancer cases and have seen a notable increase over the past 30 years. These cancers encompass various malignant tumors that... Read more