合成 DNA / RNA 类似物显示强大的抗菌活性
|
By LabMedica International staff writers Posted on 19 Nov 2013 |
|
图片：鲍氏不动杆菌的扫描电子显微镜图像，显示其基因组（外圈）和异型岛序列（内圈，红色）（照片由美国疾病控制中心的 J. Carr、以及耶鲁大学的T. Gianoulis 和 D. Massa 提供）
肽共轭的磷酰二胺吗啉代寡核苷酸 (PPMO) 是合成的 DNR/RNA 类似物，是特定基因的静默表达，研究发现 PPMO 体外和体内均能抑制不动杆菌的生长。
俄勒冈州立大（美国俄勒冈州科瓦利斯）的研究人员试图确定 PPMO 体外和体内时是否以鲁氏不动杆菌和鲍氏不动杆菌的必需基因为靶点。他们使用药物最低抑菌浓度 (MIC) 和免疫活性评估了 PPMO 的体外培养物，并使用鼻内 PPMO 治疗的小鼠肺感染模型进行了体内评估。
研究结果已发表于 2013 年 10 月 14 日的网络版《传染病期刊》(Journal of Infectious Diseases)，揭示最有效的 PPMO 检测项目为 (RXR) 4-AcpP，后者以 acpP 基因为靶点。PPMO 降低鲁氏不动杆菌和鲍氏不动杆菌的免疫活性，每毫升形成 1000 多个菌落单位，MIC 为 5-8 倍。(RXR)4-AcpP 治疗的小鼠存活时间更长，比乱序对照 PPMO 或磷酸盐缓冲盐治疗的小鼠炎症少，且细菌肺负担小。治疗可以延迟至感染后，而仍能增高存活率。
“PPMO 杀死细菌的作用机理引起了巨大的变革，”第一作者，俄勒冈州立大的微生物学教授 Bruce Geller 说。“他们几乎可以被合成以任何基因为靶点的序列，并以此避免产生抗生素耐药性，并避免产生广谱抗生素相关的负面影响。”
Related Links:
俄勒冈州立大（美国俄勒冈州科瓦利斯）的研究人员试图确定 PPMO 体外和体内时是否以鲁氏不动杆菌和鲍氏不动杆菌的必需基因为靶点。他们使用药物最低抑菌浓度 (MIC) 和免疫活性评估了 PPMO 的体外培养物，并使用鼻内 PPMO 治疗的小鼠肺感染模型进行了体内评估。
研究结果已发表于 2013 年 10 月 14 日的网络版《传染病期刊》(Journal of Infectious Diseases)，揭示最有效的 PPMO 检测项目为 (RXR) 4-AcpP，后者以 acpP 基因为靶点。PPMO 降低鲁氏不动杆菌和鲍氏不动杆菌的免疫活性，每毫升形成 1000 多个菌落单位，MIC 为 5-8 倍。(RXR)4-AcpP 治疗的小鼠存活时间更长，比乱序对照 PPMO 或磷酸盐缓冲盐治疗的小鼠炎症少，且细菌肺负担小。治疗可以延迟至感染后，而仍能增高存活率。
“PPMO 杀死细菌的作用机理引起了巨大的变革，”第一作者，俄勒冈州立大的微生物学教授 Bruce Geller 说。“他们几乎可以被合成以任何基因为靶点的序列，并以此避免产生抗生素耐药性，并避免产生广谱抗生素相关的负面影响。”
Related Links:
Gold Member
Collection and Transport System
PurSafe Plus®
Gel Cards
DG Gel Cards
Urine Chemistry Control
Dropper Urine Chemistry Control
Latest Biotech News
Channels
Clinical Chemistry
view channel
New PSA-Based Prognostic Model Improves Prostate Cancer Risk Assessment
Prostate cancer is the second-leading cause of cancer death among American men, and about one in eight will be diagnosed in their lifetime. Screening relies on blood levels of prostate-specific antigen... Read more
Extracellular Vesicles Linked to Heart Failure Risk in CKD Patients
Chronic kidney disease (CKD) affects more than 1 in 7 Americans and is strongly associated with cardiovascular complications, which account for more than half of deaths among people with CKD.... Read more
Molecular Diagnostics
view channel
Diagnostic Device Predicts Treatment Response for Brain Tumors Via Blood Test
Glioblastoma is one of the deadliest forms of brain cancer, largely because doctors have no reliable way to determine whether treatments are working in real time. Assessing therapeutic response currently... Read more
Blood Test Detects Early-Stage Cancers by Measuring Epigenetic Instability
Early-stage cancers are notoriously difficult to detect because molecular changes are subtle and often missed by existing screening tools. Many liquid biopsies rely on measuring absolute DNA methylation... Read more
“Lab-On-A-Disc” Device Paves Way for More Automated Liquid Biopsies
Extracellular vesicles (EVs) are tiny particles released by cells into the bloodstream that carry molecular information about a cell’s condition, including whether it is cancerous. However, EVs are highly... Read more
Blood Test Identifies Inflammatory Breast Cancer Patients at Increased Risk of Brain Metastasis
Brain metastasis is a frequent and devastating complication in patients with inflammatory breast cancer, an aggressive subtype with limited treatment options. Despite its high incidence, the biological... Read moreHematology
view channel
New Guidelines Aim to Improve AL Amyloidosis Diagnosis
Light chain (AL) amyloidosis is a rare, life-threatening bone marrow disorder in which abnormal amyloid proteins accumulate in organs. Approximately 3,260 people in the United States are diagnosed... Read more
Fast and Easy Test Could Revolutionize Blood Transfusions
Blood transfusions are a cornerstone of modern medicine, yet red blood cells can deteriorate quietly while sitting in cold storage for weeks. Although blood units have a fixed expiration date, cells from... Read more
Automated Hemostasis System Helps Labs of All Sizes Optimize Workflow
High-volume hemostasis sections must sustain rapid turnaround while managing reruns and reflex testing. Manual tube handling and preanalytical checks can strain staff time and increase opportunities for error.... Read more
High-Sensitivity Blood Test Improves Assessment of Clotting Risk in Heart Disease Patients
Blood clotting is essential for preventing bleeding, but even small imbalances can lead to serious conditions such as thrombosis or dangerous hemorrhage. In cardiovascular disease, clinicians often struggle... Read moreImmunology
view channel
Blood Test Identifies Lung Cancer Patients Who Can Benefit from Immunotherapy Drug
Small cell lung cancer (SCLC) is an aggressive disease with limited treatment options, and even newly approved immunotherapies do not benefit all patients. While immunotherapy can extend survival for some,... Read more
Whole-Genome Sequencing Approach Identifies Cancer Patients Benefitting From PARP-Inhibitor Treatment
Targeted cancer therapies such as PARP inhibitors can be highly effective, but only for patients whose tumors carry specific DNA repair defects. Identifying these patients accurately remains challenging,... Read more
Ultrasensitive Liquid Biopsy Demonstrates Efficacy in Predicting Immunotherapy Response
Immunotherapy has transformed cancer treatment, but only a small proportion of patients experience lasting benefit, with response rates often remaining between 10% and 20%. Clinicians currently lack reliable... Read more
Microbiology
view channel
Comprehensive Review Identifies Gut Microbiome Signatures Associated With Alzheimer’s Disease
Alzheimer’s disease affects approximately 6.7 million people in the United States and nearly 50 million worldwide, yet early cognitive decline remains difficult to characterize. Increasing evidence suggests... Read more
AI-Powered Platform Enables Rapid Detection of Drug-Resistant C. Auris Pathogens
Infections caused by the pathogenic yeast Candida auris pose a significant threat to hospitalized patients, particularly those with weakened immune systems or those who have invasive medical devices.... Read more
Pathology
view channel
Engineered Yeast Cells Enable Rapid Testing of Cancer Immunotherapy
Developing new cancer immunotherapies is a slow, costly, and high-risk process, particularly for CAR T cell treatments that must precisely recognize cancer-specific antigens. Small differences in tumor... Read more
First-Of-Its-Kind Test Identifies Autism Risk at Birth
Autism spectrum disorder is treatable, and extensive research shows that early intervention can significantly improve cognitive, social, and behavioral outcomes. Yet in the United States, the average age... Read more
Technology
view channel
Robotic Technology Unveiled for Automated Diagnostic Blood Draws
Routine diagnostic blood collection is a high‑volume task that can strain staffing and introduce human‑dependent variability, with downstream implications for sample quality and patient experience.... Read more
ADLM Launches First-of-Its-Kind Data Science Program for Laboratory Medicine Professionals
Clinical laboratories generate billions of test results each year, creating a treasure trove of data with the potential to support more personalized testing, improve operational efficiency, and enhance patient care.... Read more
Aptamer Biosensor Technology to Transform Virus Detection
Rapid and reliable virus detection is essential for controlling outbreaks, from seasonal influenza to global pandemics such as COVID-19. Conventional diagnostic methods, including cell culture, antigen... Read more
AI Models Could Predict Pre-Eclampsia and Anemia Earlier Using Routine Blood Tests
Pre-eclampsia and anemia are major contributors to maternal and child mortality worldwide, together accounting for more than half a million deaths each year and leaving millions with long-term health complications.... Read moreIndustry
view channel
New Collaboration Brings Automated Mass Spectrometry to Routine Laboratory Testing
Mass spectrometry is a powerful analytical technique that identifies and quantifies molecules based on their mass and electrical charge. Its high selectivity, sensitivity, and accuracy make it indispensable... Read more
AI-Powered Cervical Cancer Test Set for Major Rollout in Latin America
Noul Co., a Korean company specializing in AI-based blood and cancer diagnostics, announced it will supply its intelligence (AI)-based miLab CER cervical cancer diagnostic solution to Mexico under a multi‑year... Read more
Diasorin and Fisher Scientific Enter into US Distribution Agreement for Molecular POC Platform
Diasorin (Saluggia, Italy) has entered into an exclusive distribution agreement with Fisher Scientific, part of Thermo Fisher Scientific (Waltham, MA, USA), for the LIAISON NES molecular point-of-care... Read more