木瓜叶提取物可以减慢癌细胞培养物的生长
|
By LabMedica International staff writers Posted on 12 Apr 2010 |
|
癌症研究人员发现，使用一种从干木瓜叶中制备而来的水提取物对10多种不同种类肿瘤细胞培养物进行试验时，有很强的抗癌特性。
佛罗里达大学(Gainesville, FL, USA; www.ufl.edu)和东京大学(Japan; www.u-tokyo.ac.jp)的研究人员试图利用科学来研究木瓜的抗癌特性。他们的计划是，研究一种水提取的木瓜叶成份对各种肿瘤细胞株生长的作用和对人体淋巴细胞抗肿瘤作用的影响。另外，他们还尝试找出木瓜叶提取物中的功能分子量分值。在这项研究中，木瓜叶提取物对肿瘤细胞株和人体外周血单个核细胞(PBMC)的增生反应的作用以及对PBMC细胞毒素活性的作用是通过使用放射性胸腺嘧啶来进行评估的。他们还进行了流式细胞检测分析和半胱氨酸天冬酶-3/7活性测量来证实肿瘤细胞发生了细胞凋亡。PBMC产生的细胞因子是通过ELISA来衡量的。叶子提取物治疗效果的基因图谱是通过微阵列分析和实时RT-PCR进行的。
公布在2010年2月17号《民族药理学杂志》上的结果揭示出，木瓜叶提取物对宫颈癌，乳腺癌，肝，肺，胰腺肿瘤细胞株有明显生长抑制活性的作用。在PBMC中，加入木瓜叶提取物后白细胞介素-2(IL-2)和IL-4的生成减少，同时，IL-12p40, IL-12p70, IFN-γ（干扰素-γ）和TNF-α（肿瘤坏死因子－α）的生长增加，没有受到生长抑制。另外，活性PBMC的细胞毒性在加入提取物后增加。而且，微阵列分析显示，23种免疫调节基因的表达，根据基因本体论分析分类，在加入木瓜叶提取物后升高。
研究人员识别出了木瓜叶提取物的活性成份，会抑制肿瘤细胞生长和刺激抗肿瘤作用，这种成份的分子量小于1000。重要的是，木瓜提取物不会对正常细胞有任何有毒作用。
"根据我在临床情况中所看到的和所听到的，没有人使用这种提取物出现明显的毒副作用；似乎可以使用很长时间只要这种提取物有效的话"，佛罗里达大学血液学和肿瘤学教授、供稿作者Nam Dang博士说。
Related Links:
佛罗里达大学www.ufl.edu
东京大学www.u-tokyo.ac.jp
佛罗里达大学(Gainesville, FL, USA; www.ufl.edu)和东京大学(Japan; www.u-tokyo.ac.jp)的研究人员试图利用科学来研究木瓜的抗癌特性。他们的计划是，研究一种水提取的木瓜叶成份对各种肿瘤细胞株生长的作用和对人体淋巴细胞抗肿瘤作用的影响。另外，他们还尝试找出木瓜叶提取物中的功能分子量分值。在这项研究中，木瓜叶提取物对肿瘤细胞株和人体外周血单个核细胞(PBMC)的增生反应的作用以及对PBMC细胞毒素活性的作用是通过使用放射性胸腺嘧啶来进行评估的。他们还进行了流式细胞检测分析和半胱氨酸天冬酶-3/7活性测量来证实肿瘤细胞发生了细胞凋亡。PBMC产生的细胞因子是通过ELISA来衡量的。叶子提取物治疗效果的基因图谱是通过微阵列分析和实时RT-PCR进行的。
公布在2010年2月17号《民族药理学杂志》上的结果揭示出，木瓜叶提取物对宫颈癌，乳腺癌，肝，肺，胰腺肿瘤细胞株有明显生长抑制活性的作用。在PBMC中，加入木瓜叶提取物后白细胞介素-2(IL-2)和IL-4的生成减少，同时，IL-12p40, IL-12p70, IFN-γ（干扰素-γ）和TNF-α（肿瘤坏死因子－α）的生长增加，没有受到生长抑制。另外，活性PBMC的细胞毒性在加入提取物后增加。而且，微阵列分析显示，23种免疫调节基因的表达，根据基因本体论分析分类，在加入木瓜叶提取物后升高。
研究人员识别出了木瓜叶提取物的活性成份，会抑制肿瘤细胞生长和刺激抗肿瘤作用，这种成份的分子量小于1000。重要的是，木瓜提取物不会对正常细胞有任何有毒作用。
"根据我在临床情况中所看到的和所听到的，没有人使用这种提取物出现明显的毒副作用；似乎可以使用很长时间只要这种提取物有效的话"，佛罗里达大学血液学和肿瘤学教授、供稿作者Nam Dang博士说。
Related Links:
佛罗里达大学www.ufl.edu
东京大学www.u-tokyo.ac.jp
Gold Member
Flocked Fiber Swabs
Puritan® Patented HydraFlock®
New
FOB+Transferrin+Calprotectin+Lactoferrin Test
CerTest FOB+Transferrin+Calprotectin+Lactoferrin Combo Test
New
Calprotectin Assay
Fecal Calprotectin ELISA
Latest Biotech News
Channels
Clinical Chemistry
view channel
AI-Powered Blood Test Accurately Detects Ovarian Cancer
Ovarian cancer ranks as the fifth leading cause of cancer-related deaths in women, largely due to late-stage diagnoses. Although over 90% of women exhibit symptoms in Stage I, only 20% are diagnosed in... Read more
Automated Decentralized cfDNA NGS Assay Identifies Alterations in Advanced Solid Tumors
Current circulating cell-free DNA (cfDNA) assays are typically centralized, requiring specialized handling and transportation of samples. Introducing a flexible, decentralized sequencing system at the... Read more
Mass Spectrometry Detects Bacteria Without Time-Consuming Isolation and Multiplication
Speed and accuracy are essential when diagnosing diseases. Traditionally, diagnosing bacterial infections involves the labor-intensive process of isolating pathogens and cultivating bacterial cultures,... Read more
First Comprehensive Syphilis Test to Definitively Diagnose Active Infection In 10 Minutes
In the United States, syphilis cases have surged by nearly 80% from 2018 to 2023, with 209,253 cases recorded in the most recent year of data. Syphilis, which can be transmitted sexually or from mother... Read moreMolecular Diagnostics
view channel
POC Oral Swab Test to Increase Chances of Pregnancy in IVF
Approximately 15% of couples of reproductive age experience involuntary childlessness. A significant reason for this is the growing trend of delaying family planning, a global shift that is expected to... Read more
Microbial Cell-Free DNA Test Accurately Identifies Pathogens Causing Pneumonia and Other Lung Infections
Bronchoalveolar lavage (BAL) is a commonly used procedure for diagnosing lung infections, especially in immunocompromised patients. However, standard tests often fail to pinpoint the exact pathogen, leading... Read more
Hematology
view channel
First Point-of-Care Heparin Monitoring Test Provides Results in Under 15 Minutes
Heparin dosing requires careful management to avoid both bleeding and clotting complications. In high-risk situations like extracorporeal membrane oxygenation (ECMO), mortality rates can reach about 50%,... Read more
New Scoring System Predicts Risk of Developing Cancer from Common Blood Disorder
Clonal cytopenia of undetermined significance (CCUS) is a blood disorder commonly found in older adults, characterized by mutations in blood cells and a low blood count, but without any obvious cause or... Read more
Immunology
view channel
Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer
Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more
Machine Learning-Enabled Blood Test Predicts Immunotherapy Response in Lymphoma Patients
Chimeric antigen receptor (CAR) T-cell therapy has emerged as one of the most promising recent developments in the treatment of blood cancers. However, over half of non-Hodgkin lymphoma (NHL) patients... Read more
Microbiology
view channel
New Blood Test Detects Up to Five Infectious Diseases at POC
Researchers have developed a prototype flow-through assay capable of detecting up to five different infections, with results that can be quickly analyzed and transmitted via a specialized smartphone app.... Read more
Molecular Stool Test Shows Potential for Diagnosing TB in Adults with HIV
Tuberculosis (TB), caused by the bacterium Mycobacterium tuberculosis, led to 1.25 million deaths in 2023, with 13% of those occurring in people living with HIV. The current primary diagnostic method for... Read more
Pathology
view channel
Groundbreaking Chest Pain Triage Algorithm to Transform Cardiac Care
Cardiovascular disease is responsible for a third of all deaths worldwide, and chest pain is the second most common reason for emergency department (ED) visits. With EDs often being some of the busiest... Read more
AI-Based Liquid Biopsy Approach to Revolutionize Brain Cancer Detection
Detecting brain cancers remains extremely challenging, with many patients only receiving a diagnosis at later stages after symptoms like headaches, seizures, or cognitive issues appear. Late-stage diagnoses... Read more
Technology
view channel
Advanced Predictive Algorithms Identify Patients Having Undiagnosed Cancer
Two newly developed advanced predictive algorithms leverage a person’s health conditions and basic blood test results to accurately predict the likelihood of having an undiagnosed cancer, including ch... Read more
Light Signature Algorithm to Enable Faster and More Precise Medical Diagnoses
Every material or molecule interacts with light in a unique way, creating a distinct pattern, much like a fingerprint. Optical spectroscopy, which involves shining a laser on a material and observing how... Read more
Disposable Microchip Technology Could Selectively Detect HIV in Whole Blood Samples
As of the end of 2023, approximately 40 million people globally were living with HIV, and around 630,000 individuals died from AIDS-related illnesses that same year. Despite a substantial decline in deaths... Read more
Pain-On-A-Chip Microfluidic Device Determines Types of Chronic Pain from Blood Samples
Chronic pain is a widespread condition that remains difficult to manage, and existing clinical methods for its treatment rely largely on self-reporting, which can be subjective and especially problematic... Read moreIndustry
view channel
Qiagen Acquires NGS Analysis Software Company Genoox
QIAGEN (Venlo, the Netherlands) has signed a definitive agreement to acquire Genoox (Tel Aviv, Israel), a provider of artificial intelligence (AI)-powered software that enables clinical labs to scale and... Read more
Cepheid and Oxford Nanopore Technologies Partner on Advancing Automated Sequencing-Based Solutions
Cepheid (Sunnyvale, CA, USA), a leading molecular diagnostics company, and Oxford Nanopore Technologies (Oxford, UK), the company behind a new generation of sequencing-based molecular analysis technologies,... Read more
Grifols and Tecan’s IBL Collaborate on Advanced Biomarker Panels
Grifols (Barcelona, Spain), one of the world’s leading producers of plasma-derived medicines and innovative diagnostic solutions, is expanding its offer in clinical diagnostics through a strategic partnership... Read more