Clinical Relevance of Biomarkers Evaluated for Malaria
|
By LabMedica International staff writers Posted on 31 Jul 2013 |
|
Image: Malaria parasites infect two blood cells (Photo courtesy of Lennart Nilsson).
Early diagnosis and risk stratification is important in patients with Plasmodium falciparum malaria for rapid initiation of antimalarial treatment and prevention of complications.
Routine laboratory values do not correlate well with disease severity and therefore it is important to determine the diagnostic and prognostic value of several biomarkers related to inflammation; endothelial and cardiac dysfunction; coagulation, and hemolysis.
Scientists at the University Medical Center Hamburg-Eppendorf (Germany) carried out a prospective case-control study of 79 adult travelers with both uncomplicated and complicated P. falciparum malaria between 2007 and 2011; 41 healthy subjects were included as controls. Blood samples were obtained within 24 hours after first consultation to assess routine laboratory values as well as markers related to inflammation, plasma protein-A (PAPP-A), copeptin, C-reactive protein (CRP); endothelial activation, myeloperoxidase (MPO), elastase-2, endothelin-1, soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular adhesion molecule-1 (sVCAM-1); cardiac function, N-terminal pro-brain natriuretic peptide (NT-proBNP), midregional pro-atrial natriuretic peptide (MR-proANP); coagulation, fibrinogen, D-dimers, platelet count; and hemolysis, lactate dehydrogenase (LDH).
Twelve (15.2%) patients had severe P. falciparum malaria. In the patient group, significant thrombocytopenia was found, all other markers were significantly elevated except for PAPP-A. Median values of CRP, MPO, sICAM-1, sVCAM-1, D-dimers, platelets, and LDH differed significantly between the two groups. However further analysis via receiver operating curve (AUROC) showed large 95% confidence intervals (CI), indicating poor association with disease severity for all markers. No significant association was found for copeptin, elastase-2, or fibrinogen. The performance was best for CRP, followed by MPO, D-dimers, elastase-2, and sICAM-1, but biomarker levels did not correlate well with disease severity. Some commercially available immunofluorescence assays were provided by Thermo Scientific (Hennigsdorf, Germany).
The authors concluded that the combination of travel history, fever prior to blood sampling, and CRP serum levels above or below 10.8 mg/L upon hospital admission, best discriminated between malaria patients and control individuals. None of the biomarkers studied predicted the presence or the development of malaria complications, neither at the time of admission nor during hospitalization. The study was published on July 16, 2013, in the Malaria Journal.
Related Links:
University Medical Center Hamburg-Eppendorf
Thermo Scientific
Routine laboratory values do not correlate well with disease severity and therefore it is important to determine the diagnostic and prognostic value of several biomarkers related to inflammation; endothelial and cardiac dysfunction; coagulation, and hemolysis.
Scientists at the University Medical Center Hamburg-Eppendorf (Germany) carried out a prospective case-control study of 79 adult travelers with both uncomplicated and complicated P. falciparum malaria between 2007 and 2011; 41 healthy subjects were included as controls. Blood samples were obtained within 24 hours after first consultation to assess routine laboratory values as well as markers related to inflammation, plasma protein-A (PAPP-A), copeptin, C-reactive protein (CRP); endothelial activation, myeloperoxidase (MPO), elastase-2, endothelin-1, soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular adhesion molecule-1 (sVCAM-1); cardiac function, N-terminal pro-brain natriuretic peptide (NT-proBNP), midregional pro-atrial natriuretic peptide (MR-proANP); coagulation, fibrinogen, D-dimers, platelet count; and hemolysis, lactate dehydrogenase (LDH).
Twelve (15.2%) patients had severe P. falciparum malaria. In the patient group, significant thrombocytopenia was found, all other markers were significantly elevated except for PAPP-A. Median values of CRP, MPO, sICAM-1, sVCAM-1, D-dimers, platelets, and LDH differed significantly between the two groups. However further analysis via receiver operating curve (AUROC) showed large 95% confidence intervals (CI), indicating poor association with disease severity for all markers. No significant association was found for copeptin, elastase-2, or fibrinogen. The performance was best for CRP, followed by MPO, D-dimers, elastase-2, and sICAM-1, but biomarker levels did not correlate well with disease severity. Some commercially available immunofluorescence assays were provided by Thermo Scientific (Hennigsdorf, Germany).
The authors concluded that the combination of travel history, fever prior to blood sampling, and CRP serum levels above or below 10.8 mg/L upon hospital admission, best discriminated between malaria patients and control individuals. None of the biomarkers studied predicted the presence or the development of malaria complications, neither at the time of admission nor during hospitalization. The study was published on July 16, 2013, in the Malaria Journal.
Related Links:
University Medical Center Hamburg-Eppendorf
Thermo Scientific
Gold Member
Chagas Disease Test
CHAGAS Cassette
New
Auto Clinical Chemistry Analyzer
cobas c 703
New
Vaginitis Test
Allplex Vaginitis Screening Assay
Latest Clinical Chem. News
- Carbon Nanotubes Help Build Highly Accurate Sensors for Continuous Health Monitoring
- Paper-Based Device Boosts HIV Test Accuracy from Dried Blood Samples
- AI-Powered Raman Spectroscopy Method Enables Rapid Drug Detection in Blood
- Novel LC-MS/MS Assay Detects Low Creatinine in Sweat and Saliva
- Biosensing Technology Breakthrough Paves Way for New Methods of Early Disease Detection
- New Saliva Test Rapidly Identifies Paracetamol Overdose
- POC Saliva Testing Device Predicts Heart Failure in 15 Minutes
- Screening Tool Detects Multiple Health Conditions from Single Blood Drop
- Integrated Chemistry and Immunoassay Analyzer with Extensive Assay Menu Offers Flexibility, Scalability and Data Commutability
- Rapid Drug Test to Improve Treatment for Patients Presenting to Hospital
- AI Model Detects Cancer at Lightning Speed through Sugar Analyses
- First-Ever Blood-Powered Chip Offers Real-Time Health Monitoring
- New ADLM Guidance Provides Expert Recommendations on Clinical Testing For Respiratory Viral Infections
- 3D Printed Point-Of-Care Mass Spectrometer Outperforms State-Of-The-Art Models
- POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection
- Highly Reliable Cell-Based Assay Enables Accurate Diagnosis of Endocrine Diseases
Channels
Molecular Diagnostics
view channel
RNA-Based Blood Test Detects Preeclampsia Risk Months Before Symptoms
Preeclampsia remains a major cause of maternal morbidity and mortality, as well as preterm births. Despite current guidelines that aim to identify pregnant women at increased risk of preeclampsia using... Read more
First Of Its Kind Test Uses microRNAs to Predict Toxicity from Cancer Therapy
Many men with early-stage prostate cancer receive stereotactic body radiotherapy (SBRT), a highly precise form of radiation treatment that is completed in just five sessions. Compared to traditional radiation,... Read more
Novel Cell-Based Assay Provides Sensitive and Specific Autoantibody Detection in Demyelination
Anti-myelin-associated glycoprotein (MAG) antibodies serve as markers for an autoimmune demyelinating disorder that affects the peripheral nervous system, leading to sensory impairment. Anti-MAG-IgM antibodies... Read more
Hematology
view channel
New Scoring System Predicts Risk of Developing Cancer from Common Blood Disorder
Clonal cytopenia of undetermined significance (CCUS) is a blood disorder commonly found in older adults, characterized by mutations in blood cells and a low blood count, but without any obvious cause or... Read more
Non-Invasive Prenatal Test for Fetal RhD Status Demonstrates 100% Accuracy
In the United States, approximately 15% of pregnant individuals are RhD-negative. However, in about 40% of these cases, the fetus is also RhD-negative, making the administration of RhoGAM unnecessary.... Read more
Immunology
view channel
Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer
Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more
Machine Learning-Enabled Blood Test Predicts Immunotherapy Response in Lymphoma Patients
Chimeric antigen receptor (CAR) T-cell therapy has emerged as one of the most promising recent developments in the treatment of blood cancers. However, over half of non-Hodgkin lymphoma (NHL) patients... Read more
Microbiology
view channel
Handheld Device Deliver Low-Cost TB Results in Less Than One Hour
Tuberculosis (TB) remains the deadliest infectious disease globally, affecting an estimated 10 million people annually. In 2021, about 4.2 million TB cases went undiagnosed or unreported, mainly due to... Read more
New AI-Based Method Improves Diagnosis of Drug-Resistant Infections
Drug-resistant infections, particularly those caused by deadly bacteria like tuberculosis and staphylococcus, are rapidly emerging as a global health emergency. These infections are more difficult to treat,... Read more
Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours
Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Pathology
view channel
Advanced Imaging Reveals Mechanisms Causing Autoimmune Disease
Myasthenia gravis, an autoimmune disease, leads to muscle weakness that can affect a range of muscles, including those needed for basic actions like blinking, smiling, or moving. Researchers have long... Read more
AI Model Effectively Predicts Patient Outcomes in Common Lung Cancer Type
Lung adenocarcinoma, the most common form of non-small cell lung cancer (NSCLC), typically adopts one of six distinct growth patterns, often combining multiple patterns within a single tumor.... Read more
Technology
view channel
Pain-On-A-Chip Microfluidic Device Determines Types of Chronic Pain from Blood Samples
Chronic pain is a widespread condition that remains difficult to manage, and existing clinical methods for its treatment rely largely on self-reporting, which can be subjective and especially problematic... Read more
Innovative, Label-Free Ratiometric Fluorosensor Enables More Sensitive Viral RNA Detection
Viruses present a major global health risk, as demonstrated by recent pandemics, making early detection and identification essential for preventing new outbreaks. While traditional detection methods are... Read more
Industry
view channel
Cepheid and Oxford Nanopore Technologies Partner on Advancing Automated Sequencing-Based Solutions
Cepheid (Sunnyvale, CA, USA), a leading molecular diagnostics company, and Oxford Nanopore Technologies (Oxford, UK), the company behind a new generation of sequencing-based molecular analysis technologies,... Read more
Grifols and Tecan’s IBL Collaborate on Advanced Biomarker Panels
Grifols (Barcelona, Spain), one of the world’s leading producers of plasma-derived medicines and innovative diagnostic solutions, is expanding its offer in clinical diagnostics through a strategic partnership... Read more
