Stem Cell Therapy is Safe: Reprogramming Does Not Increase the Rate of Mutagenesis
By LabMedica International staff writers Posted on 22 Feb 2017 |
Image: Induced pluripotent stem cells (iPSCs) are the basis of a technique that opens the doors to many medical advances involving repair and restoration of tissues and organs (Photo courtesy of Darryl Leja, National Human Genome Research Institute).
DNA mapping studies have shown that the genomes of induced pluripotent stem cells do not contain more mutations that did the original cell line from which they were generated.
One important unsolved question in the stem cell field is, do induced pluripotent stem cells (iPSCs) have more mutations than other cultured somatic cells because of the reprogramming process? The delay in answering this question has slowed adoption of iPSCs in biomedical research and medicine, as concerns that these cells are prone to increased numbers of genetic mutations persist.
Investigators at the [U.S.] National Human Genome Research Institute adopted a unique approach to answering the question of the possible increase in mutation rate in iSPCs by deriving fibroblast subclones and clonal iPSC lines from the same fibroblast population. This provided the investigators with parallel cell lines from the same source, only one of which having undergone the process to turn them into iPSCs.
The investigators applied next-generation sequencing to compare genomic variations in these lines. They reported in the February 6, 2017, online edition of the journal Proceedings of the [U.S.] National Academy of Sciences that targeted deep sequencing of parental fibroblasts revealed that most variants detected in clonal iPSCs and fibroblast subclones were rare variants inherited from the parental fibroblasts. More than 90% of these mutations were random, preexisting sequence variants in small subsets of the parental fibroblast population. This data strongly suggested that reprogramming process was not mutagenic.
"This technology will eventually change how doctors treat diseases. These findings suggest that the question of safety should not impede research using iPSC," said senior author Dr. Paul Liu, senior investigator in the Translational and Functional Genomics Branch of the [U.S.] National Human Genome Research Institute.
One important unsolved question in the stem cell field is, do induced pluripotent stem cells (iPSCs) have more mutations than other cultured somatic cells because of the reprogramming process? The delay in answering this question has slowed adoption of iPSCs in biomedical research and medicine, as concerns that these cells are prone to increased numbers of genetic mutations persist.
Investigators at the [U.S.] National Human Genome Research Institute adopted a unique approach to answering the question of the possible increase in mutation rate in iSPCs by deriving fibroblast subclones and clonal iPSC lines from the same fibroblast population. This provided the investigators with parallel cell lines from the same source, only one of which having undergone the process to turn them into iPSCs.
The investigators applied next-generation sequencing to compare genomic variations in these lines. They reported in the February 6, 2017, online edition of the journal Proceedings of the [U.S.] National Academy of Sciences that targeted deep sequencing of parental fibroblasts revealed that most variants detected in clonal iPSCs and fibroblast subclones were rare variants inherited from the parental fibroblasts. More than 90% of these mutations were random, preexisting sequence variants in small subsets of the parental fibroblast population. This data strongly suggested that reprogramming process was not mutagenic.
"This technology will eventually change how doctors treat diseases. These findings suggest that the question of safety should not impede research using iPSC," said senior author Dr. Paul Liu, senior investigator in the Translational and Functional Genomics Branch of the [U.S.] National Human Genome Research Institute.
Latest BioResearch News
- Genome Analysis Predicts Likelihood of Neurodisability in Oxygen-Deprived Newborns
- Gene Panel Predicts Disease Progession for Patients with B-cell Lymphoma
- New Method Simplifies Preparation of Tumor Genomic DNA Libraries
- New Tool Developed for Diagnosis of Chronic HBV Infection
- Panel of Genetic Loci Accurately Predicts Risk of Developing Gout
- Disrupted TGFB Signaling Linked to Increased Cancer-Related Bacteria
- Gene Fusion Protein Proposed as Prostate Cancer Biomarker
- NIV Test to Diagnose and Monitor Vascular Complications in Diabetes
- Semen Exosome MicroRNA Proves Biomarker for Prostate Cancer
- Genetic Loci Link Plasma Lipid Levels to CVD Risk
- Newly Identified Gene Network Aids in Early Diagnosis of Autism Spectrum Disorder
- Link Confirmed between Living in Poverty and Developing Diseases
- Genomic Study Identifies Kidney Disease Loci in Type I Diabetes Patients
- Liquid Biopsy More Effective for Analyzing Tumor Drug Resistance Mutations
- New Liquid Biopsy Assay Reveals Host-Pathogen Interactions
- Method Developed for Enriching Trophoblast Population in Samples