Crystal Structure Determined for Binding Site of Zika/Dengue Neutralizing Antibodies
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By LabMedica International staff writers Posted on 05 Jul 2016 |
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Image: A model of the three-dimensional structure of the Zika virus envelope protein (red, yellow and blue) in complex with the neutralizing antibody (in green and white) (Photo courtesy of the Institut Pasteur).
An international team of molecular virologists has identified neutralizing antibodies that bind to and block both dengue virus and Zika virus, which may pave the way for development of a vaccine to protect against both viruses simultaneously.
The international team, headed by investigators at Institut Pasteur (Paris, France), examined the effect of two antibodies isolated from dengue patients on the Zika virus.
They reported in the June 23, 2016, online edition of the journal Nature that one of the antibodies was even better at neutralizing the Zika virus than it was at neutralizing dengue virus. The X-ray crystal structure of the antibody in complex with the envelope protein of Zika virus revealed details of a conserved epitope, which was also the site of interaction of the envelope protein dimer with the precursor prM protein during virus maturation. This binding site on the Zika virus is the same as the one on the dengue virus, raising the possibility of producing a vaccine that would stimulate the production of antibodies capable of binding to and neutralizing both types of virus at the same time.
"We wanted to see whether the antibodies isolated for dengue could be used to neutralize other flaviviruses, and Zika seemed like the best candidate," said senior author Dr. Félix Rey, head of the structural virology laboratory at the Institut Pasteur. "We never expected to discover that the dengue virus and the Zika virus are so close that some antibodies produced against the dengue virus could also neutralize the Zika virus so potently. The antibodies could be used, for example, to protect pregnant women at risk of contracting the Zika virus, because there is currently no vaccine or treatment for this disease."
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Institut Pasteur
The international team, headed by investigators at Institut Pasteur (Paris, France), examined the effect of two antibodies isolated from dengue patients on the Zika virus.
They reported in the June 23, 2016, online edition of the journal Nature that one of the antibodies was even better at neutralizing the Zika virus than it was at neutralizing dengue virus. The X-ray crystal structure of the antibody in complex with the envelope protein of Zika virus revealed details of a conserved epitope, which was also the site of interaction of the envelope protein dimer with the precursor prM protein during virus maturation. This binding site on the Zika virus is the same as the one on the dengue virus, raising the possibility of producing a vaccine that would stimulate the production of antibodies capable of binding to and neutralizing both types of virus at the same time.
"We wanted to see whether the antibodies isolated for dengue could be used to neutralize other flaviviruses, and Zika seemed like the best candidate," said senior author Dr. Félix Rey, head of the structural virology laboratory at the Institut Pasteur. "We never expected to discover that the dengue virus and the Zika virus are so close that some antibodies produced against the dengue virus could also neutralize the Zika virus so potently. The antibodies could be used, for example, to protect pregnant women at risk of contracting the Zika virus, because there is currently no vaccine or treatment for this disease."
Related Links:
Institut Pasteur
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