New Instrument Automates Droplet Generation for Digital Droplet PCR Systems
By LabMedica International staff writers Posted on 09 Nov 2014 |
Biotech researchers and other life scientists working with PCR techniques will be interested in the availability of a new instrument that automates and standardizes droplet generation for droplet digital PCR (ddPRC).
Bio-Rad Laboratories, Inc. (Hercules, CA, USA) has launched its Automated Droplet Generator (AutoDG Instrument) that complements the QX100 and QX200 Droplet Readers. This combination of instruments and a laptop computer equipped with the QuantaSoft software package constitute the automated droplet digital PCR system.
The AutoDG Instrument creates droplets quickly, reproducibly, and reliably. At maximum capacity, the system can generate droplets for 96 wells in less than 45 minutes. The instrument can generate droplets for fluorescent probe-based or EvaGreen dye-based detection.
The Droplet Generator is used to partition the ddPCR reaction mix into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the Droplet Reader. Droplets are sipped, and the singulator unpacks the emulsified droplets and streams them in single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.
The AutoDG Instrument has its own hood and HEPA filter, reducing contamination so the system can be used on a standard laboratory bench, without the need for a PCR cabinet or cleanroom.
Bio-Rad's digital biology center marketing managers said, "With a quick setup, you are able to walk away and return to a full plate of droplets ready for thermal cycling and analysis. Droplet Digital PCR is sensitive enough to detect differences in people’s pipetting habits. This instrument eliminates that variability."
Related Links:
Bio-Rad Laboratories, Inc.
Bio-Rad Laboratories, Inc. (Hercules, CA, USA) has launched its Automated Droplet Generator (AutoDG Instrument) that complements the QX100 and QX200 Droplet Readers. This combination of instruments and a laptop computer equipped with the QuantaSoft software package constitute the automated droplet digital PCR system.
The AutoDG Instrument creates droplets quickly, reproducibly, and reliably. At maximum capacity, the system can generate droplets for 96 wells in less than 45 minutes. The instrument can generate droplets for fluorescent probe-based or EvaGreen dye-based detection.
The Droplet Generator is used to partition the ddPCR reaction mix into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the Droplet Reader. Droplets are sipped, and the singulator unpacks the emulsified droplets and streams them in single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.
The AutoDG Instrument has its own hood and HEPA filter, reducing contamination so the system can be used on a standard laboratory bench, without the need for a PCR cabinet or cleanroom.
Bio-Rad's digital biology center marketing managers said, "With a quick setup, you are able to walk away and return to a full plate of droplets ready for thermal cycling and analysis. Droplet Digital PCR is sensitive enough to detect differences in people’s pipetting habits. This instrument eliminates that variability."
Related Links:
Bio-Rad Laboratories, Inc.
Read the full article by registering today, it's FREE!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
- Free digital version edition of LabMedica International sent by email on regular basis
- Free print version of LabMedica International magazine (available only outside USA and Canada).
- Free and unlimited access to back issues of LabMedica International in digital format
- Free LabMedica International Newsletter sent every week containing the latest news
- Free breaking news sent via email
- Free access to Events Calendar
- Free access to LinkXpress new product services
- REGISTRATION IS FREE AND EASY!
Sign in: Registered website members
Sign in: Registered magazine subscribers
Latest BioResearch News
- Genome Analysis Predicts Likelihood of Neurodisability in Oxygen-Deprived Newborns
- Gene Panel Predicts Disease Progession for Patients with B-cell Lymphoma
- New Method Simplifies Preparation of Tumor Genomic DNA Libraries
- New Tool Developed for Diagnosis of Chronic HBV Infection
- Panel of Genetic Loci Accurately Predicts Risk of Developing Gout
- Disrupted TGFB Signaling Linked to Increased Cancer-Related Bacteria
- Gene Fusion Protein Proposed as Prostate Cancer Biomarker
- NIV Test to Diagnose and Monitor Vascular Complications in Diabetes
- Semen Exosome MicroRNA Proves Biomarker for Prostate Cancer
- Genetic Loci Link Plasma Lipid Levels to CVD Risk
- Newly Identified Gene Network Aids in Early Diagnosis of Autism Spectrum Disorder
- Link Confirmed between Living in Poverty and Developing Diseases
- Genomic Study Identifies Kidney Disease Loci in Type I Diabetes Patients
- Liquid Biopsy More Effective for Analyzing Tumor Drug Resistance Mutations
- New Liquid Biopsy Assay Reveals Host-Pathogen Interactions
- Method Developed for Enriching Trophoblast Population in Samples