Simple Method Corrects Automated Hemoglobin Measurement Interference
|
By LabMedica International staff writers Posted on 30 Oct 2013 |
|
Image: Normal blood sample on the left and blood sample containing high triglycerides on the right (Photo courtesy of Prof. Tisha Joy, MD).
Hypertriglyceridemia is a well-known factor that can interfere with the measurement of hemoglobin (HGB) using automated hematology analyzers and it can cause falsely high HGB values.
A simple but effective correction method has been introduced to rectify the interference of high blood triglyceride (TG) levels in measurement of blood HGB by automated hematology analyzers commonly used in clinical laboratories.
Clinical laboratorians at the Sichuan University (Chengdu, China) analyzed 50 whole blood samples and 50 plasma samples containing variable TG concentrations. Complete blood cell counts (CBCs) were performed by an XE-2100 automated hematology analyzer (Sysmex; Kobe, Japan) for 102 blood samples, in which high-level TG were artificially added. The same blood samples were centrifuged at different speeds to separate the plasma from blood cells. An optimal centrifugal force and time were identified to separate plasma and blood cells and to exert the least influence on the chylomicrons (CMs) in the plasma, which was then analyzed.
By using the two CBC results, a correction formula was established to calculate the corrected HGB, mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) values. Comparisons were also made of HGB, MCH, and MCHC values before and after correction of in-patient individuals who received intralipid and developed lipemia. The percentage differences between the corrected and true values of HGB, MCH and MCHC were −0.28%, 0.06%, and −0.31%, respectively. The correlation coefficients of corrected values versus true values of HGB, MCH, and MCHC were 0.989, 0.935, and 0.717, respectively. This correction method was also effective for native lipemic samples.
The authors concluded that by adding a simple step of low-speed centrifugation, their method was able to provide reliable results of HGB, MCH, and MCHC in highly lipemic blood samples without special equipment. This provided evidence that the HGB concentration of intralipid and plasma mixture was not affected by different centrifugal conditions. Therefore, they recommended that the least time-consuming centrifugation parameter be adopted. The study was published on September 13, 2013, in the Journal of Clinical Laboratory Analysis.
Related Links:
Sichuan University
Sysmex
A simple but effective correction method has been introduced to rectify the interference of high blood triglyceride (TG) levels in measurement of blood HGB by automated hematology analyzers commonly used in clinical laboratories.
Clinical laboratorians at the Sichuan University (Chengdu, China) analyzed 50 whole blood samples and 50 plasma samples containing variable TG concentrations. Complete blood cell counts (CBCs) were performed by an XE-2100 automated hematology analyzer (Sysmex; Kobe, Japan) for 102 blood samples, in which high-level TG were artificially added. The same blood samples were centrifuged at different speeds to separate the plasma from blood cells. An optimal centrifugal force and time were identified to separate plasma and blood cells and to exert the least influence on the chylomicrons (CMs) in the plasma, which was then analyzed.
By using the two CBC results, a correction formula was established to calculate the corrected HGB, mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) values. Comparisons were also made of HGB, MCH, and MCHC values before and after correction of in-patient individuals who received intralipid and developed lipemia. The percentage differences between the corrected and true values of HGB, MCH and MCHC were −0.28%, 0.06%, and −0.31%, respectively. The correlation coefficients of corrected values versus true values of HGB, MCH, and MCHC were 0.989, 0.935, and 0.717, respectively. This correction method was also effective for native lipemic samples.
The authors concluded that by adding a simple step of low-speed centrifugation, their method was able to provide reliable results of HGB, MCH, and MCHC in highly lipemic blood samples without special equipment. This provided evidence that the HGB concentration of intralipid and plasma mixture was not affected by different centrifugal conditions. Therefore, they recommended that the least time-consuming centrifugation parameter be adopted. The study was published on September 13, 2013, in the Journal of Clinical Laboratory Analysis.
Related Links:
Sichuan University
Sysmex
Gold Member
Flocked Fiber Swabs
Puritan® Patented HydraFlock®
New
Centrifuge
Hematocrit Centrifuge 7511M4
New
Bordetella Pertussis Molecular Assay
Alethia Pertussis
Latest Hematology News
- New Scoring System Predicts Risk of Developing Cancer from Common Blood Disorder
- Non-Invasive Prenatal Test for Fetal RhD Status Demonstrates 100% Accuracy
- WBC Count Could Predict Severity of COVID-19 Symptoms
- New Platelet Counting Technology to Help Labs Prevent Diagnosis Errors
- Streamlined Approach to Testing for Heparin-Induced Thrombocytopenia Improves Diagnostic Accuracy
- POC Hemostasis System Could Help Prevent Maternal Deaths
- New Test Assesses Oxygen Delivering Ability of Red Blood Cells by Measuring Their Shape
- Personalized CBC Testing Could Help Diagnose Early-Stage Diseases in Healthy Individuals
- Non-Invasive Test Solution Determines Fetal RhD Status from Maternal Plasma
- First-Of-Its-Kind Smartphone Technology Noninvasively Measures Blood Hemoglobin Levels at POC
- Next Gen CBC and Sepsis Diagnostic System Targets Faster, Earlier, Easier Results
- Newly Discovered Blood Group System to Help Identify and Treat Rare Patients
- Blood Platelet Score Detects Previously Unmeasured Risk of Heart Attack and Stroke
- Automated Benchtop System to Bring Blood Testing To Anyone, Anywhere
- New Hematology Analyzers Deliver Combined ESR and CBC/DIFF Results in 60 Seconds
- Next Generation Instrument Screens for Hemoglobin Disorders in Newborns
Channels
Clinical Chemistry
view channel
Carbon Nanotubes Help Build Highly Accurate Sensors for Continuous Health Monitoring
Current sensors can measure various health indicators, such as blood glucose levels, in the body. However, there is a need to develop more accurate and sensitive sensor materials that can detect lower... Read more
Paper-Based Device Boosts HIV Test Accuracy from Dried Blood Samples
In regions where access to clinics for routine blood tests presents financial and logistical obstacles, HIV patients are increasingly able to collect and send a drop of blood using paper-based devices... Read more
Molecular Diagnostics
view channel
Novel Point-of-Care Technology Delivers Accurate HIV Results in Minutes
HIV diagnostic methods have traditionally relied on detecting HIV-specific antibodies, which typically appear weeks after infection. This delayed detection has hindered early diagnosis, complicating patient... Read more
Blood Test Rules Out Future Dementia Risk
Previous studies have suggested that specific biomarkers, such as tau217, Neurofilament Light (NfL), and Glial Fibrillary Acidic Protein (GFAP), may be valuable for early dementia diagnosis.... Read more
Immunology
view channel
Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer
Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more
Machine Learning-Enabled Blood Test Predicts Immunotherapy Response in Lymphoma Patients
Chimeric antigen receptor (CAR) T-cell therapy has emerged as one of the most promising recent developments in the treatment of blood cancers. However, over half of non-Hodgkin lymphoma (NHL) patients... Read more
Microbiology
view channel
New AI-Based Method Improves Diagnosis of Drug-Resistant Infections
Drug-resistant infections, particularly those caused by deadly bacteria like tuberculosis and staphylococcus, are rapidly emerging as a global health emergency. These infections are more difficult to treat,... Read more
Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours
Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Innovative ID/AST System to Help Diagnose Infectious Diseases and Combat AMR
Each year, 11 million people across the world die of sepsis out of which 1.3 million deaths are due to antibiotic-resistant bacteria. The burden of antimicrobial resistance (AMR) continues to weigh heavily,... Read more
Gastrointestinal Panel Delivers Rapid Detection of Five Common Bacterial Pathogens for Outpatient Use
Acute infectious gastroenteritis results in approximately 179 million cases each year in the United States, leading to a significant number of outpatient visits and hospitalizations. To address this, a... Read morePathology
view channel
AI Model Predicts Patient Response to Bladder Cancer Treatment
Each year in the United States, around 81,000 new cases of bladder cancer are diagnosed, leading to approximately 17,000 deaths annually. Muscle-invasive bladder cancer (MIBC) is a severe form of bladder... Read more
New Laser-Based Method to Accelerate Cancer Diagnosis
Researchers have developed a method to improve cancer diagnostics and other diseases. Collagen, a key structural protein, plays various roles in cell activity. A novel multidisciplinary study published... Read more
New AI Model Predicts Gene Variants’ Effects on Specific Diseases
In recent years, artificial intelligence (AI) has greatly enhanced our ability to identify a vast number of genetic variants in increasingly larger populations. However, up to half of these variants are... Read more
Powerful AI Tool Diagnoses Coeliac Disease from Biopsy Images with Over 97% Accuracy
Coeliac disease is an autoimmune disorder triggered by the consumption of gluten, causing symptoms such as stomach cramps, diarrhea, skin rashes, weight loss, fatigue, and anemia. Due to the wide variation... Read moreTechnology
view channel
Innovative, Label-Free Ratiometric Fluorosensor Enables More Sensitive Viral RNA Detection
Viruses present a major global health risk, as demonstrated by recent pandemics, making early detection and identification essential for preventing new outbreaks. While traditional detection methods are... Read more
Smartphones Could Diagnose Diseases Using Infrared Scans
Rapid advancements in technology may soon make it possible for individuals to bypass invasive medical procedures by simply uploading a screenshot of their lab results from their phone directly to their doctor.... Read more
Novel Sensor Technology to Enable Early Diagnoses of Metabolic and Cardiovascular Disorders
Metabolites are critical compounds that fuel life's essential functions, playing a key role in producing energy, regulating cellular activities, and maintaining the balance of bodily systems.... Read more
3D Printing Breakthrough Enables Large Scale Development of Tiny Microfluidic Devices
Microfluidic devices are diagnostic systems capable of analyzing small volumes of materials with precision and speed. These devices are used in a variety of applications, including cancer cell analysis,... Read moreIndustry
view channel
Grifols and Tecan’s IBL Collaborate on Advanced Biomarker Panels
Grifols (Barcelona, Spain), one of the world’s leading producers of plasma-derived medicines and innovative diagnostic solutions, is expanding its offer in clinical diagnostics through a strategic partnership... Read more
