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Microbiology

Image: Lauco crystal violet (LCV) colorimetric detection results of limit of detection (LoD) tests for the primer sets used for SARS-CoV-2 detection. 20U/reaction of reverse transcriptase were used (Photo courtesy of Korea Research Institute of Chemical Technology).

RT-LAMP Assay Developed for SARS-CoV-2

SARS-CoV-2 is the causative viral pathogen of COVID-19, and diagnosis of COVID-19 can be done through CT scan of suspicious patients and a confirmatory laboratory test is performed using published real-time quantitative polymerase chain reaction (RT-qPCR) methods. More...
20 Apr 2020
Image: An enzyme-linked immunosorbent assay (ELISA) kit for the diagnosis of visceral leishmaniasis in humans (Photo courtesy of Bordier Affinity Products)

Commercial Kits Evaluated for Mediterranean Visceral Leishmaniasis

Human visceral leishmaniasis (VL) is a neglected disease endemic in the Mediterranean region, caused by Leishmania infantum. Accurate VL diagnosis is critical for rapid and correct patient management. Dogs are the main reservoir of L. infantum parasites that are transmitted to humans by the bite of infected phlebotomine sand flies. More...
16 Apr 2020
DIASOURCE (A Biovendor Company)
Image: Colored transmission electron microscopy of the coronavirus. SARS-CoV-2–positive sputum and feces was found after conversion of pharyngeal samples in patients with COVID-19. This virus was isolated from the fecal matter of a patient with diarrhea (Photo courtesy of BSIP/UIG)

SARS-CoV-2 Found in Sputum/Feces After Negative Pharyngeal Specimens

The outbreak of coronavirus disease 2019 (COVID-19) has become a global public health problem. In the absence of a specific therapy or vaccine, timely diagnosis and the establishment of a sufficient isolation period for infected individuals are critical to containment efforts. More...
14 Apr 2020
Image: Photomicrograph of a vaginal epithelial clue cell coated with Gardnerella vaginalis (Photo courtesy of Universidad Autónoma de Zacatecas).

Potential Microbial Markers Identified for Cervical Pre-Cancer Progression

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections. However, only a small percentage of high-risk (HR) HPV infections progress to cervical pre-cancer and cancer. The role of the cervicovaginal microbiome (CVM) in the natural history of HR-HPV has been investigated. More...
08 Apr 2020
Image: The Oxford Nanopore MinION device (Photo courtesy of Oxford Nanopore Technologies).

Deep Throat SARS-CoV-2 Viral Load Ascertained in Saliva Samples

Posterior oropharyngeal saliva samples are a non-invasive specimen more acceptable to patients and health-care workers. Unlike severe acute respiratory syndrome, patients with COVID-19 had the highest viral load near presentation, which could account for the fast-spreading nature of this epidemic. More...
06 Apr 2020
Image:  The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates (Photo courtesy of Molecular Devices).

Novel Rapid Test Developed for Detecting Carbapenemase

As a potent β-lactamase, carbapenemase can degrade almost all β-lactam antimicrobial drugs, including the carbapenems, regarded as the last line of therapy for many life-threatening infections. If uncontrolled, the spread of these carbapenemases is expected to increase therapeutic failure and leave many patients with no effective treatment options. More...
02 Apr 2020
Image: The image shows bacterial cells trapped in a three-dimensional microbead matrix (Photo courtesy of Wenrong He, Rochester Institute of Technology)

Novel Nanofluidic Device Traps and Concentrates Pathogenic Bacteria

 A nanofluidic device containing stacked magnetic beads of different sizes has been developed to efficiently trap, concentrate, and retrieve Escherichia coli (E. coli) bacteria from liquid suspensions. More...
31 Mar 2020
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The LabMedica Microbiology channel provides the latest news in the fields of epidemiology, bacteriology, virology, and parasitology, all viewed from the unique perspective of Laboratory Medicine.
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