Prothrombin Time Derived from POC Test Compared to Laboratory Test
By LabMedica International staff writers Posted on 17 Feb 2015 |
Prothrombin time/international normalized ratio (PT/INR) is used for dosing and monitoring oral anticoagulant therapy (OAT) based on warfarin, as the therapeutic range of warfarin is narrow and there is a considerable variation in inter- and intra-individual pharmacologic response.
One of the most widely used point-of-care (POC) devices to evaluate prothrombin time for monitoring oral anticoagulant therapy is unlike other laboratory methods, as it detects electrical signals produced by thrombin activity to derive the international normalized ratio (INR).
Scientists at Yonsei University College of Medicine (Seoul, Korea) collected 91 venous blood samples submitted for fibrinogen testing to the clinical laboratory. Platelet-poor plasma was obtained by centrifugation. The fibrinogen level was measured using Fibrinogen-C XL reagent (Instrumentation Laboratory; Milan, Italy) via Clauss method using ACL TOP (Beckman Coulter; Fullerton, CA, USA). Samples were selected after fibrinogen testing according to the fibrinogen level. PT INR was measured by both the laboratory method and CoaguChek XS (Roche Diagnostics; Mannheim, Germany).
The samples were stratified into low, mid, and high fibrinogen groups by fibrinogen levels of <130 mg/dL, 130–450 mg/dL, and >450 mg/dL, respectively. The mean INR difference of the low fibrinogen group was significantly different from that of the mid or high fibrinogen group. In the low fibrinogen group, CoaguChek XS INR showed a negative bias compared with the laboratory INR, while the mid and high fibrinogen groups had positive bias.
The authors concluded that that patient selection according to fibrinogen status should precede the implementation of POC testing using CoaguChek XS. Also, periodic comparisons between CoaguChek XS and laboratory INR results should be continued during the use of CoaguChek XS. The study was published on January 31, 2015, in the Journal of Clinical Laboratory Analysis.
Related Links:
Yonsei University College of Medicine
Instrumentation Laboratory
Roche Diagnostics
One of the most widely used point-of-care (POC) devices to evaluate prothrombin time for monitoring oral anticoagulant therapy is unlike other laboratory methods, as it detects electrical signals produced by thrombin activity to derive the international normalized ratio (INR).
Scientists at Yonsei University College of Medicine (Seoul, Korea) collected 91 venous blood samples submitted for fibrinogen testing to the clinical laboratory. Platelet-poor plasma was obtained by centrifugation. The fibrinogen level was measured using Fibrinogen-C XL reagent (Instrumentation Laboratory; Milan, Italy) via Clauss method using ACL TOP (Beckman Coulter; Fullerton, CA, USA). Samples were selected after fibrinogen testing according to the fibrinogen level. PT INR was measured by both the laboratory method and CoaguChek XS (Roche Diagnostics; Mannheim, Germany).
The samples were stratified into low, mid, and high fibrinogen groups by fibrinogen levels of <130 mg/dL, 130–450 mg/dL, and >450 mg/dL, respectively. The mean INR difference of the low fibrinogen group was significantly different from that of the mid or high fibrinogen group. In the low fibrinogen group, CoaguChek XS INR showed a negative bias compared with the laboratory INR, while the mid and high fibrinogen groups had positive bias.
The authors concluded that that patient selection according to fibrinogen status should precede the implementation of POC testing using CoaguChek XS. Also, periodic comparisons between CoaguChek XS and laboratory INR results should be continued during the use of CoaguChek XS. The study was published on January 31, 2015, in the Journal of Clinical Laboratory Analysis.
Related Links:
Yonsei University College of Medicine
Instrumentation Laboratory
Roche Diagnostics
Read the full article by registering today, it's FREE!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
- Free digital version edition of LabMedica International sent by email on regular basis
- Free print version of LabMedica International magazine (available only outside USA and Canada).
- Free and unlimited access to back issues of LabMedica International in digital format
- Free LabMedica International Newsletter sent every week containing the latest news
- Free breaking news sent via email
- Free access to Events Calendar
- Free access to LinkXpress new product services
- REGISTRATION IS FREE AND EASY!
Sign in: Registered website members
Sign in: Registered magazine subscribers
Latest Hematology News
- Next Generation Instrument Screens for Hemoglobin Disorders in Newborns
- First 4-in-1 Nucleic Acid Test for Arbovirus Screening to Reduce Risk of Transfusion-Transmitted Infections
- POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy
- First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy
- Handheld White Blood Cell Tracker to Enable Rapid Testing For Infections
- Smart Palm-size Optofluidic Hematology Analyzer Enables POCT of Patients’ Blood Cells
- Automated Hematology Platform Offers High Throughput Analytical Performance
- New Tool Analyzes Blood Platelets Faster, Easily and Accurately
- First Rapid-Result Hematology Analyzer Reports Measures of Infection and Severity at POC
- Bleeding Risk Diagnostic Test to Reduce Preventable Complications in Hospitals
- True POC Hematology Analyzer with Direct Capillary Sampling Enhances Ease-of-Use and Testing Throughput
- Point of Care CBC Analyzer with Direct Capillary Sampling Enhances Ease-of-Use and Testing Throughput
- Blood Test Could Predict Outcomes in Emergency Department and Hospital Admissions
- Novel Technology Diagnoses Immunothrombosis Using Breath Gas Analysis
- Advanced Hematology System Allows Labs to Process Up To 119 Complete Blood Count Results per Hour
- Unique AI-Based Approach Automates Clinical Analysis of Blood Data