Blood Biomarkers Diagnose Stroke Accurately
|
By LabMedica International staff writers Posted on 23 Apr 2014 |
|
Image: The IX71- DSU Spinning Disk Confocal inverted microscope (Photo courtesy of Olympus America).
A new blood test is being developed that one day could rapidly confirm whether someone is having a stroke and what kind, will lead to faster diagnosis and treatment, which could mean the difference between life and death.
A device has been constructed that can process whole blood and isolate genetic material for two potential stroke biomarkers within minutes, and by identifying more biomarkers could aid in diagnosis as the device can analyze a total of four biomarkers at the same time.
Scientists at the Louisiana State University (Baton Rouge, LA, USA) designed and tested the performance of a polymer microfluidic device that can affinity select multiple types of biological cells simultaneously with sufficient recovery and purity to allow for the expression profiling of messenger ribonucleic acid (mRNA) isolated from these cells.
The microfluidic device consisted of four independent selection beds with curvilinear channels that were 25 μm wide and 80 μm deep and were modified with antibodies targeting antigens specifically expressed by two different cell types. Bifurcated and Z-configured device geometries were evaluated for cell selection. Whole blood samples were obtained from anonymous healthy donors. To analyze the cells the scientists used flow cytometric analysis and evaluated carboxylic acid surface densities.
Cell characterization was performed by acquiring fluorescent images on an IX71-DSU Spinning Disk Confocal inverted microscope (Olympus America; Center Valley, PA, USA). Reverse transcription and polymerase chain reaction (PCR) of CD4+ T-cells and neutrophils was performed after they were isolated on a chip and lysed by infusing with lysis buffer. Reverse transcription (RT) was accomplished using a ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs; Ipswich, MA, USA).
The team demonstrated the affinity-based microfluidic system was capable of isolating highly pure subsets of leukocytes from whole blood. Processing 50 μL of whole blood in about three minutes provided sufficient genetic material for gene expression profiling. Special emphasis was placed on the fluid dynamics and design architecture of the device for T-cell and neutrophil isolation to obtain high purity leukocytes fractions using a single step. The isolation of two types of cells from whole blood was accomplished with purity of greater than 90%.
The expression of possible stroke biomarker genes from isolated T-cells and neutrophils, such as the calcium binding protein A9 (S100A9), the T cell receptor beta (TCRB), and the formyl peptide receptor 1 (FPR1), was evaluated using RT-PCR. The modification and isolation procedures can also be used to analyze other cell types as well where multiple subsets need to be investigated. The study was published online on March 20, 2014, in the journal Analytical Chemistry.
Related Links:
Louisiana State University
Olympus America
New England BioLabs
A device has been constructed that can process whole blood and isolate genetic material for two potential stroke biomarkers within minutes, and by identifying more biomarkers could aid in diagnosis as the device can analyze a total of four biomarkers at the same time.
Scientists at the Louisiana State University (Baton Rouge, LA, USA) designed and tested the performance of a polymer microfluidic device that can affinity select multiple types of biological cells simultaneously with sufficient recovery and purity to allow for the expression profiling of messenger ribonucleic acid (mRNA) isolated from these cells.
The microfluidic device consisted of four independent selection beds with curvilinear channels that were 25 μm wide and 80 μm deep and were modified with antibodies targeting antigens specifically expressed by two different cell types. Bifurcated and Z-configured device geometries were evaluated for cell selection. Whole blood samples were obtained from anonymous healthy donors. To analyze the cells the scientists used flow cytometric analysis and evaluated carboxylic acid surface densities.
Cell characterization was performed by acquiring fluorescent images on an IX71-DSU Spinning Disk Confocal inverted microscope (Olympus America; Center Valley, PA, USA). Reverse transcription and polymerase chain reaction (PCR) of CD4+ T-cells and neutrophils was performed after they were isolated on a chip and lysed by infusing with lysis buffer. Reverse transcription (RT) was accomplished using a ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs; Ipswich, MA, USA).
The team demonstrated the affinity-based microfluidic system was capable of isolating highly pure subsets of leukocytes from whole blood. Processing 50 μL of whole blood in about three minutes provided sufficient genetic material for gene expression profiling. Special emphasis was placed on the fluid dynamics and design architecture of the device for T-cell and neutrophil isolation to obtain high purity leukocytes fractions using a single step. The isolation of two types of cells from whole blood was accomplished with purity of greater than 90%.
The expression of possible stroke biomarker genes from isolated T-cells and neutrophils, such as the calcium binding protein A9 (S100A9), the T cell receptor beta (TCRB), and the formyl peptide receptor 1 (FPR1), was evaluated using RT-PCR. The modification and isolation procedures can also be used to analyze other cell types as well where multiple subsets need to be investigated. The study was published online on March 20, 2014, in the journal Analytical Chemistry.
Related Links:
Louisiana State University
Olympus America
New England BioLabs
Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Gold Member
Plasma Control
Plasma Control Level 1
Latest Technology News
- New Diagnostic System Achieves PCR Testing Accuracy
- DNA Biosensor Enables Early Diagnosis of Cervical Cancer
- Self-Heating Microfluidic Devices Can Detect Diseases in Tiny Blood or Fluid Samples
- Breakthrough in Diagnostic Technology Could Make On-The-Spot Testing Widely Accessible
- First of Its Kind Technology Detects Glucose in Human Saliva
- Electrochemical Device Identifies People at Higher Risk for Osteoporosis Using Single Blood Drop
- Novel Noninvasive Test Detects Malaria Infection without Blood Sample
- Portable Optofluidic Sensing Devices Could Simultaneously Perform Variety of Medical Tests
- Point-of-Care Software Solution Helps Manage Disparate POCT Scenarios across Patient Testing Locations
- Electronic Biosensor Detects Biomarkers in Whole Blood Samples without Addition of Reagents
- Breakthrough Test Detects Biological Markers Related to Wider Variety of Cancers
- Rapid POC Sensing Kit to Determine Gut Health from Blood Serum and Stool Samples
- Device Converts Smartphone into Fluorescence Microscope for Just USD 50
- Wi-Fi Enabled Handheld Tube Reader Designed for Easy Portability
Channels
Clinical Chemistry
view channel
3D Printed Point-Of-Care Mass Spectrometer Outperforms State-Of-The-Art Models
Mass spectrometry is a precise technique for identifying the chemical components of a sample and has significant potential for monitoring chronic illness health states, such as measuring hormone levels... Read more.jpg "Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)")
POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection
Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more
Highly Reliable Cell-Based Assay Enables Accurate Diagnosis of Endocrine Diseases
The conventional methods for measuring free cortisol, the body's stress hormone, from blood or saliva are quite demanding and require sample processing. The most common method, therefore, involves collecting... Read more
Molecular Diagnostics
view channel
Blood Proteins Could Warn of Cancer Seven Years before Diagnosis
Two studies have identified proteins in the blood that could potentially alert individuals to the presence of cancer more than seven years before the disease is clinically diagnosed. Researchers found... Read more (1)_1.jpg "Image: Ultrasound image of micro-histotripsy liberation of biomarkers in a tumor (Photo courtesy of Joy Wang and Pradyumna Kedarisetti)"){kind=small}
Ultrasound-Aided Blood Testing Detects Cancer Biomarkers from Cells
Ultrasound imaging serves as a noninvasive method to locate and monitor cancerous tumors effectively. However, crucial details about the cancer, such as the specific types of cells and genetic mutations... Read more_1.jpg "Image: The innovative method to customize and strengthen DNA origami structure promises breakthroughs in medicine (Photo courtesy of Shutterstock)")
.jpg "Image: The QIAstat-Dx Respiratory Panel Plus has received U.S. FDA clearance (Photo courtesy of QIAGEN)")
Hematology
view channel
Next Generation Instrument Screens for Hemoglobin Disorders in Newborns
Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more
First 4-in-1 Nucleic Acid Test for Arbovirus Screening to Reduce Risk of Transfusion-Transmitted Infections
Arboviruses represent an emerging global health threat, exacerbated by climate change and increased international travel that is facilitating their spread across new regions. Chikungunya, dengue, West... Read more
POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy
Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more
First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy
Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read moreImmunology
view channel.jpg "Image: The AI predictive model identifies the most potent cancer killing immune cells for use in immunotherapies (Photo courtesy of Shutterstock)")
AI Predicts Tumor-Killing Cells with High Accuracy
Cellular immunotherapy involves extracting immune cells from a patient's tumor, potentially enhancing their cancer-fighting capabilities through engineering, and then expanding and reintroducing them into the body.... Read more
Diagnostic Blood Test for Cellular Rejection after Organ Transplant Could Replace Surgical Biopsies
Transplanted organs constantly face the risk of being rejected by the recipient's immune system which differentiates self from non-self using T cells and B cells. T cells are commonly associated with acute... Read more
AI Tool Precisely Matches Cancer Drugs to Patients Using Information from Each Tumor Cell
Current strategies for matching cancer patients with specific treatments often depend on bulk sequencing of tumor DNA and RNA, which provides an average profile from all cells within a tumor sample.... Read more
Genetic Testing Combined With Personalized Drug Screening On Tumor Samples to Revolutionize Cancer Treatment
Cancer treatment typically adheres to a standard of care—established, statistically validated regimens that are effective for the majority of patients. However, the disease’s inherent variability means... Read moreMicrobiology
view channel
Integrated Solution Ushers New Era of Automated Tuberculosis Testing
Tuberculosis (TB) is responsible for 1.3 million deaths every year, positioning it as one of the top killers globally due to a single infectious agent. In 2022, around 10.6 million people were diagnosed... Read more
Automated Sepsis Test System Enables Rapid Diagnosis for Patients with Severe Bloodstream Infections
Sepsis affects up to 50 million people globally each year, with bacteraemia, formerly known as blood poisoning, being a major cause. In the United States alone, approximately two million individuals are... Read more
Enhanced Rapid Syndromic Molecular Diagnostic Solution Detects Broad Range of Infectious Diseases
GenMark Diagnostics (Carlsbad, CA, USA), a member of the Roche Group (Basel, Switzerland), has rebranded its ePlex® system as the cobas eplex system. This rebranding under the globally renowned cobas name... Read more
Clinical Decision Support Software a Game-Changer in Antimicrobial Resistance Battle
Antimicrobial resistance (AMR) is a serious global public health concern that claims millions of lives every year. It primarily results from the inappropriate and excessive use of antibiotics, which reduces... Read morePathology
view channel
AI Integrated With Optical Imaging Technology Enables Rapid Intraoperative Diagnosis
Rapid and accurate intraoperative diagnosis is essential for tumor surgery as it guides surgical decisions with precision. Traditional intraoperative assessments, such as frozen sections based on H&E... Read more
HPV Self-Collection Solution Improves Access to Cervical Cancer Testing
Annually, over 604,000 women across the world are diagnosed with cervical cancer, and about 342,000 die from this disease, which is preventable and primarily caused by the Human Papillomavirus (HPV).... Read more
Hyperspectral Dark-Field Microscopy Enables Rapid and Accurate Identification of Cancerous Tissues
Breast cancer remains a major cause of cancer-related mortality among women. Breast-conserving surgery (BCS), also known as lumpectomy, is the removal of the cancerous lump and a small margin of surrounding tissue.... Read more
Industry
view channel
Danaher and Johns Hopkins University Collaborate to Improve Neurological Diagnosis
Unlike severe traumatic brain injury (TBI), mild TBI often does not show clear correlations with abnormalities detected through head computed tomography (CT) scans. Consequently, there is a pressing need... Read more
Beckman Coulter and MeMed Expand Host Immune Response Diagnostics Partnership
Beckman Coulter Diagnostics (Brea, CA, USA) and MeMed BV (Haifa, Israel) have expanded their host immune response diagnostics partnership. Beckman Coulter is now an authorized distributor of the MeMed... Read more_1.jpg "Image: Thermo Fisher will distribute Bio-Techne products across Europe (Photo courtesy of 123RF)")
