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Microbiology

Image: Salmonella are gram-negative, rod-shaped, facultative anaerobic bacteria. Newly developed sensitive and specific assays are able to detect different serotypes of Salmonella, paving the way for rapid serotyping directly from specimens (Photo courtesy of Public Health Image Library, [U.S.] Centers for Disease Control and Prevention)

A Series of Sensitive and Specific DNA-Based Assays Detects and Differentiates Salmonella Serotypes

A team of Australian researchers developed a series of sensitive and specific assays to detect and identify different serotypes (serovars) of Salmonella, a common cause of bacterial foodborne disease worldwide. More...
07 May 2020
Image: Antibody Detection Is Critical for COVID-19 Diagnosis (Photo courtesy of Minnesota Department of Health).

Antibody Detection Is Critical for COVID-19 Diagnosis

Accurate and fast diagnosis of the causative SARS CoV-2 is important to isolate the patients with COVID-19 timely and stop the epidemics, as well as save people's lives. Viral nucleic acid detection using real-time polymerase chain reaction (RT-PCR) assay, which has been developed and used for detection of SARS-CoV-2 rapidly, remains the standard diagnosis of COVID-19. More...
06 May 2020
Image: The positive results of Helicobacter pylori infection by the traditional Giemsa stain. White arrows indicate stained Helicobacter pylori (blue) that are attached to the brush border of the gastric foveolar epithelial cells (Photo courtesy of National Health Research Institutes).

Giemsa Stain Outperforms Urease Test for Helicobacter Pylori Diagnosis

More than 50% of the worldwide population is infected by Helicobacter pylori which is harbored in their upper gastrointestinal tract. Despite having chronic gastritis, most people infected by H. pylori are asymptomatic and have no specific clinical signs and symptoms. More...
05 May 2020
Image: The LightCycler 96 Instrument is a real-time PCR system for rapid cycling up to 96 samples (Photo courtesy of Roche Molecular Systems).

Scabies Detected by Molecular Methods in Skin Scrapings

Scabies caused by Sarcoptes scabiei mites, is a highly contagious parasitic disease characterized by intense itching which is aggravated at night. Infections by scabies mites result in various skin eruptions consisting of papules, nodules, vesicles, and excoriated eczematous lesions due to scratching. More...
29 Apr 2020
Image: The PHOMO Microplate Reader: the sophisticated optical design combined with the accurate plate transport system allows for high resolution scanning (29 points per well) to support agglutination assays (Photo courtesy of Autobio Diagnostics Co).

Urea Dissociation Tests Reduces SARS-CoV-2 IgM False-Positives

At present, the diagnosis of COVID-19 is mainly based on epidemiological history inquiry, laboratory testing, and chest radiology examination. Among these examinations, the detection of nucleic acid from SARS-CoV-2 is the direct evidence for COVID-19 diagnosis. More...
28 Apr 2020
Image: The Abbott RealTime CT/NG assay provides detection of Chlamydia trachomatis and Neisseria gonorrhoeae with proven clinical performance in sexually transmitted disease testing (Photo courtesy of Abbott Laboratories).

Real-Time Assay Evaluated for Two Sexually Transmitted Diseases

Chlamydia trachomatis and Neisseria gonorrhoeae are the two most commonly reported sexually transmitted infections (STIs) in the USA and the numbers of cases are increasing. C. trachomatis and N. gonorrhoeae infections in the rectum and pharynx are important extragenital reservoirs of infection. More...
21 Apr 2020
Image: Lauco crystal violet (LCV) colorimetric detection results of limit of detection (LoD) tests for the primer sets used for SARS-CoV-2 detection. 20U/reaction of reverse transcriptase were used (Photo courtesy of Korea Research Institute of Chemical Technology).

RT-LAMP Assay Developed for SARS-CoV-2

SARS-CoV-2 is the causative viral pathogen of COVID-19, and diagnosis of COVID-19 can be done through CT scan of suspicious patients and a confirmatory laboratory test is performed using published real-time quantitative polymerase chain reaction (RT-qPCR) methods. More...
20 Apr 2020
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