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Molecular Diagnostics

Image: The ProSpecT Rotavirus Microplate ELISA Assay (Photo courtesy of Thermo Fisher Scientific)

Rotavirus Co-Infection with Diarrheagenic Pathogens Detected by Different Methods

Diarrhea is the second most common cause of childhood mortality worldwide, with Group A Rotavirus (RVA) recognized as a leading diarrheagenic agent, causing 38% of acute diarrhea among children aged five and below worldwide. More...
14 Jul 2021
Image: (c) Illustration of workflow for human buccal swab sample testing on the toroidal PCR. Total turnaround time is under one hour. (d) Genotyping 15 SNP loci from buccal swab samples from a family trio (Photo courtesy of Rice University)

Portable, PCR-Based Assay Developed for Rapid, Multiplexed DNA Detection

The detection of specific DNA sequences is central to precision medicine, from pathogen identification to the risk assessment of human genetic diseases to disease prognosis. While technologies exist for DNA detection, they tend to be limited either in terms of multiplexing, turnaround times, quantification accuracy, or specificity for single-nucleotide differences. More...
13 Jul 2021
Image: A normal bone marrow biopsy: Note the presence of an eosinophilic myelocyte, a basophilic myelocyte, and a plasma cell. There is an association between a common, benign genotype and unnecessary bone marrow biopsies among African-American patients (Photo courtesy of University of Utah School of Medicine)

Genotyping Helps Avoid Needless Bone Marrow Biopsies in African-Americans

Bone marrow biopsies may be used unnecessarily in some African American individuals who are genetically predisposed to having relatively low white blood cell counts. More...
13 Jul 2021
Image: Artist’s depiction of stress molecules in blood being detected electronically inside nanowells (Photo courtesy of Ella Marushchenko)

A Novel Nanowell Array Sensor for POC Measurement of Stress Hormones

Researchers have constructed a microchip "nanosensor" to measure stress hormones such as cortisol in a dedicated nanowell array, which does not require molecular labels or washing steps. More...
13 Jul 2021
Image: Micrograph showing inflammation of the large bowel in a case of inflammatory bowel disease (Photo courtesy of Wikimedia Commons)

Novel Stool Biomarker Screening Tests Detect Colorectal Cancer and Inflammatory Bowel Disease

Two recent papers described the use of novel stool protein biomarkers in screening tests to diagnose colorectal cancer (CRC) and inflammatory bowel disease (IBD). More...
12 Jul 2021
Image: The proximity extension assay identified 19 plasma hub proteins (indicated as yellow dots in the figure) in AD patients, which were irregular compared to healthy people (Photo courtesy of Hong Kong University of Science and Technology)

Protein Biomarker Blood Test Accurately Detects Alzheimer’s Disease

A 19-protein biomarker panel distinguished Alzheimer’s disease (AD) patients from healthy individuals with greater than 96% accuracy and differentiated among the early, intermediate, and late stages of AD while monitoring the progression of the disease. More...
07 Jul 2021
Image: Micrograph image of human papilloma virus associated oropharyngeal cancer (HPV+ OPSCC). The tissue was stained to show the presence of the virus by in situ hybridization (Photo courtesy of Wikimedia Commons)

Sensitive PCR Test Predicts Treatment Response in Metastatic Head and Neck Cancers

A recently developed droplet digital PCR (ddPCR)-based assay for circulating tumor DNA (ctDNA) from human papillomavirus HPV16 (which is responsible for almost 90% of HPV-positive oropharyngeal cancers) predicts treatment response in metastatic human papillomavirus related (HPV+) oropharyngeal squamous cell carcinoma (OPSCC). More...
06 Jul 2021
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