Platelet Indices Analyzed for Tuberculosis and Diabetes Mellitus Co-Morbidity
|
By LabMedica International staff writers Posted on 03 Jun 2021 |
|
Image: The Mindray BC-6800 hematology analyzer (Photo courtesy of Shenzhen Mindray Bio-Medical Electronics)
Studies have demonstrated that some diseases accelerate Tuberculosis (TB) occurrence and development. Type 2 diabetes mellitus (DM) has been verified as one of the threatening risk factors for TB, and patients have three times the risk of developing TB compared to non-diabetic patients due to pathogenic mechanisms and metabolic factors.
Platelets are enucleate cells and have critical roles in thrombosis, homeostasis and the inflammatory response. When the internal environment is changed, platelet morphology may be altered and play a role in certain platelet-associated parameters, mainly including platelet count (PC), plateletcrit (PCT), platelet distribution width (PDW) and mean platelet volume (MPV).
Clinical Laboratorians at Nantong University College of Medicine (Nantong, China) enrolled in a study 246 patients admitted to the hospital and were distributed into three groups (113 TB, 59 DM and 74 TB + DM). A total of 133 individuals were also recruited as healthy controls (HC). Platelet indices, namely, platelet count (PC), mean platelet volume (MPV), plateletcrit (PCT) and platelet distribution width (PDW), were compared among the four groups, and the relationship with inflammatory markers was explored by using statistical software.
TB diagnose was based on positive results of Xpert MTB/RIF (Cepheid Inc., Sunnyvale, CA, USA). The team used BACTEC MGIT 960 rapid liquid isolate culture (Becton Dickinson, Sparks, MD, USA), and the GenoType check system (Hain Lifescience, Nehren, Germany), and MTB smear confirmation by Ziehl-Neelsen acid-fast stain. Full blood counts were carried out using Mindray BC-6800 hematology analyzer (Shenzhen Mindray Bio-Medical Electronics Co. Ltd., Shenzhen, China). The Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were also measured.
The scientists reported that MPV and PCT were significantly downregulated in TB + DM patients (9.95 ± 1.25 fL, 0.20 ± 0.05%, separately) compared with DM individuals (10.92 ± 1.17 fL, 0.22 ± 0.04%). Moreover, the changes in MPV were significantly higher in TB + DM patients (9.95 ± 1.25 fL) than in TB patients (9.42 ± 1.01 fL). MPV improved the diagnosis sensitivity when it was combined with clinical parameters, such as fasting blood glucose in DM and Mycobacterium tuberculosis culture result in TB (76.3% versus 64.9%, 72.6% versus 60.8%), respectively. In addition, the sensitivity and specificity of PCT in the differential diagnosis of DM patients versus TB + DM patients were 69.5 and 59.4%, respectively. PCT improved the diagnosis sensitivity when combined with fasting blood glucose in DM (72.9% versus 64.9%). In addition, MPV was linked to CRP and ESR in the TB + DM patients.
The authors concluded that MPV is a valuable candidate marker to screen for TB-DM coinfection risk, as the occurrence of TB developing into TB-DM coinfection will increase MPV levels, and DM developing into a DM-TB coinfection will decrease MPV levels. MPV has significant specificity and sensitivity for predicting and diagnosing DM-TB coinfection. The study was published on May 20, 2021 in the journal BMC Infectious Diseases.
Related Links:
Nantong University College of Medicine
Cepheid Inc
Becton Dickinson
Hain Lifescience
Shenzhen Mindray
Platelets are enucleate cells and have critical roles in thrombosis, homeostasis and the inflammatory response. When the internal environment is changed, platelet morphology may be altered and play a role in certain platelet-associated parameters, mainly including platelet count (PC), plateletcrit (PCT), platelet distribution width (PDW) and mean platelet volume (MPV).
Clinical Laboratorians at Nantong University College of Medicine (Nantong, China) enrolled in a study 246 patients admitted to the hospital and were distributed into three groups (113 TB, 59 DM and 74 TB + DM). A total of 133 individuals were also recruited as healthy controls (HC). Platelet indices, namely, platelet count (PC), mean platelet volume (MPV), plateletcrit (PCT) and platelet distribution width (PDW), were compared among the four groups, and the relationship with inflammatory markers was explored by using statistical software.
TB diagnose was based on positive results of Xpert MTB/RIF (Cepheid Inc., Sunnyvale, CA, USA). The team used BACTEC MGIT 960 rapid liquid isolate culture (Becton Dickinson, Sparks, MD, USA), and the GenoType check system (Hain Lifescience, Nehren, Germany), and MTB smear confirmation by Ziehl-Neelsen acid-fast stain. Full blood counts were carried out using Mindray BC-6800 hematology analyzer (Shenzhen Mindray Bio-Medical Electronics Co. Ltd., Shenzhen, China). The Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were also measured.
The scientists reported that MPV and PCT were significantly downregulated in TB + DM patients (9.95 ± 1.25 fL, 0.20 ± 0.05%, separately) compared with DM individuals (10.92 ± 1.17 fL, 0.22 ± 0.04%). Moreover, the changes in MPV were significantly higher in TB + DM patients (9.95 ± 1.25 fL) than in TB patients (9.42 ± 1.01 fL). MPV improved the diagnosis sensitivity when it was combined with clinical parameters, such as fasting blood glucose in DM and Mycobacterium tuberculosis culture result in TB (76.3% versus 64.9%, 72.6% versus 60.8%), respectively. In addition, the sensitivity and specificity of PCT in the differential diagnosis of DM patients versus TB + DM patients were 69.5 and 59.4%, respectively. PCT improved the diagnosis sensitivity when combined with fasting blood glucose in DM (72.9% versus 64.9%). In addition, MPV was linked to CRP and ESR in the TB + DM patients.
The authors concluded that MPV is a valuable candidate marker to screen for TB-DM coinfection risk, as the occurrence of TB developing into TB-DM coinfection will increase MPV levels, and DM developing into a DM-TB coinfection will decrease MPV levels. MPV has significant specificity and sensitivity for predicting and diagnosing DM-TB coinfection. The study was published on May 20, 2021 in the journal BMC Infectious Diseases.
Related Links:
Nantong University College of Medicine
Cepheid Inc
Becton Dickinson
Hain Lifescience
Shenzhen Mindray
Gold Member
Quantitative POC Immunoassay Analyzer
EASY READER+
6 Part Hematology Analyzer with RET + IPF
Mispa HX 88
Gold Member
Immunochromatographic Assay
CRYPTO Cassette
Latest Clinical Chem. News
- VOCs Show Promise for Early Multi-Cancer Detection
- Portable Raman Spectroscopy Offers Cost-Effective Kidney Disease Diagnosis at POC
- Gold Nanoparticles to Improve Accuracy of Ovarian Cancer Diagnosis
- Simultaneous Cell Isolation Technology Improves Cancer Diagnostic Accuracy
- Simple Non-Invasive Hair-Based Test Could Speed ALS Diagnosis
- Paper Strip Saliva Test Detects Elevated Uric Acid Levels Without Blood Draws
- Prostate Cancer Markers Based on Chemical Make-Up of Calcifications to Speed Up Detection
- Breath Test Could Help Detect Blood Cancers
- ML-Powered Gas Sensors to Detect Pathogens and AMR at POC
- Saliva-Based Cancer Detection Technology Eliminates Need for Complex Sample Preparation
- Skin Swabs Could Detect Parkinson’s Years Before Symptoms Appear
- New Clinical Chemistry Analyzer Designed to Meet Growing Demands of Modern Labs
- New Reference Measurement Procedure Standardizes Nucleic Acid Amplification Test Results
- Pen-Like Tool Quickly and Non-Invasively Detects Opioids from Skin
- Simple Urine Test Could Detect Multiple Cancers at Early Stage
- Earwax Test Accurately Detects Parkinson’s by Identifying Odor Molecules
Channels
Clinical Chemistry
view channel
VOCs Show Promise for Early Multi-Cancer Detection
Early cancer detection is critical to improving survival rates, but most current screening methods focus on individual cancer types and often involve invasive procedures. This makes it difficult to identify... Read more
Portable Raman Spectroscopy Offers Cost-Effective Kidney Disease Diagnosis at POC
Kidney disease is typically diagnosed through blood or urine tests, often when patients present with symptoms such as blood in urine, shortness of breath, or weight loss. While these tests are common,... Read more
Molecular Diagnostics
view channel
New Diagnostic Method Detects Pneumonia at POC in Low-Resource Settings
Pneumonia continues to be one of the leading causes of death in low- and middle-income countries, where limited access to advanced laboratory infrastructure hampers early and accurate diagnosis.... Read more
Blood Immune Cell Analysis Detects Parkinson’s Before Symptoms Appear
Early diagnosis of Parkinson’s disease remains one of the greatest challenges in neurology. The condition, which affects nearly 12 million people globally, is typically identified only after significant... Read more
Immunology
view channel
Blood-Based Liquid Biopsy Model Analyzes Immunotherapy Effectiveness
Immunotherapy has revolutionized cancer care by harnessing the immune system to fight tumors, yet predicting who will benefit remains a major challenge. Many patients undergo costly and taxing treatment... Read more
Signature Genes Predict T-Cell Expansion in Cancer Immunotherapy
Modern cancer immunotherapies rely on the ability of CD8⁺ T cells to rapidly multiply within tumors, generating the immune force needed to eliminate cancer cells. However, the biological triggers behind... Read more
Microbiology
view channel
High-Throughput Enteric Panels Detect Multiple GI Bacterial Infections from Single Stool Swab Sample
Gastrointestinal (GI) infections are among the most common causes of illness worldwide, leading to over 1.7 million deaths annually and placing a heavy burden on healthcare systems. Conventional diagnostic... Read more
Fast Noninvasive Bedside Test Uses Sugar Fingerprint to Detect Fungal Infections
Candida bloodstream infections are a growing global health threat, causing an estimated 6 million cases and 3.8 million deaths annually. Hospitals are particularly vulnerable, as weakened patients after... Read more
Pathology
view channel
New Molecular Analysis Tool to Improve Disease Diagnosis
Accurately distinguishing between similar biomolecules such as proteins is vital for biomedical research and diagnostics, yet existing analytical tools often fail to detect subtle structural or compositional... Read more
Tears Offer Noninvasive Alternative for Diagnosing Neurodegenerative Diseases
Diagnosing and monitoring eye and neurodegenerative diseases often requires invasive procedures to access ocular fluids. Ocular fluids like aqueous humor and vitreous humor contain valuable molecular information... Read more
Technology
view channel
Cell-Sorting Device Uses Electromagnetic Levitation to Precisely Direct Cell Movement
Sorting different cell types—such as cancerous versus healthy or live versus dead cells—is a critical task in biology and medicine. However, conventional methods often require labeling, chemical exposure,... Read more
Embedded GPU Platform Enables Rapid Blood Profiling for POC Diagnostics
Blood tests remain a cornerstone of medical diagnostics, but traditional imaging and analysis methods can be slow, costly, and reliant on dyes or contrast agents. Now, scientists have developed a real-time,... Read more
Industry
view channel
Qiagen Acquires Single-Cell Omics Firm Parse Biosciences
QIAGEN (Venlo, Netherlands) has entered into a definitive agreement to fully acquire Parse Biosciences (Seattle, WA, USA), a provider of scalable, instrument-free solutions for single-cell research.... Read more
Puritan Medical Products Showcasing Innovation at AMP2025 in Boston
Puritan Medical Products (Guilford, ME, USA), the world’s most trusted manufacturer of swabs and specimen collection devices, is set to exhibit at AMP2025 in Boston, Massachusetts, from November 11–15.... Read more
Advanced Instruments Merged Under Nova Biomedical Name
Advanced Instruments (Norwood, MA, USA) and Nova Biomedical (Waltham, MA, USA) are now officially doing business under a single, unified brand. This transformation is expected to deliver greater value... Read more
