Reliable Diagnostic Test Developed for Scabies
By LabMedica International staff writers Posted on 22 Nov 2011 |
A fluoroimmunoassay has been developed to determine the specific antibodies in the blood of patients infested with the human itch mite, Sarcoptes scabiei.
Scabies antigen specific immunoglobulin E (IgE) was measured by a dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) and quantified, while a competitive inhibition enzyme-linked immunosorbent assay (ELISA) was also conducted.
Scientists from the Menzies School of Health Research, (Darwin, NT, Australia) collaborated with other institutions, to create recombinant allergens to S. scabiei and house dust mites (HDM). Specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups. The 140 consenting volunteers consisted of six groups: 30 subjects with crusted scabies; 30 subjects with ordinary scabies; 30 previously exposed but currently noninfested subjects; 30 subjects naïve to scabies; 10 naïve subjects with atopic allergy as defined by positive skin prick test to a panel of common aeroallergens; and 10 naïve subjects with known allergy to human dust mites based on clinical history and positive specific IgE.
The mean IgE binding to the recombinant antigen for the crusted scabies group was significantly greater compared to each of the other groups. The ordinary scabies group also had a significantly higher mean value than each noninfested group, and the exposed but noninfested group's mean IgE response was significantly greater than the mean response for the naïve to scabies group. The recombinant antigen DELFIA (Wallac; Turku, Finland) showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects.
The authors concluded that the results demonstrate sensitive detection of IgE reactivity with genus-specific scabies mite epitopes and differential diagnosis of scabies mite allergy from allergy to HDM. The recombinant antigen specific IgE DELFIA has high diagnostic efficiency, with accuracy superior to other diagnostic methods currently available for scabies. Due to the high sensitivity, specificity, accuracy, predictive values, and area under the ROC curve, the developed DELFIA represents a marked improvement for the clinical diagnosis of scabies and helps direct future development of a specific diagnostic tool for scabies. The study was published on October 21, 2011, in the journal Diagnostic Microbiology and Infectious Disease.
Related Links:
Menzies School of Health Research
Wallac
Scabies antigen specific immunoglobulin E (IgE) was measured by a dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) and quantified, while a competitive inhibition enzyme-linked immunosorbent assay (ELISA) was also conducted.
Scientists from the Menzies School of Health Research, (Darwin, NT, Australia) collaborated with other institutions, to create recombinant allergens to S. scabiei and house dust mites (HDM). Specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups. The 140 consenting volunteers consisted of six groups: 30 subjects with crusted scabies; 30 subjects with ordinary scabies; 30 previously exposed but currently noninfested subjects; 30 subjects naïve to scabies; 10 naïve subjects with atopic allergy as defined by positive skin prick test to a panel of common aeroallergens; and 10 naïve subjects with known allergy to human dust mites based on clinical history and positive specific IgE.
The mean IgE binding to the recombinant antigen for the crusted scabies group was significantly greater compared to each of the other groups. The ordinary scabies group also had a significantly higher mean value than each noninfested group, and the exposed but noninfested group's mean IgE response was significantly greater than the mean response for the naïve to scabies group. The recombinant antigen DELFIA (Wallac; Turku, Finland) showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects.
The authors concluded that the results demonstrate sensitive detection of IgE reactivity with genus-specific scabies mite epitopes and differential diagnosis of scabies mite allergy from allergy to HDM. The recombinant antigen specific IgE DELFIA has high diagnostic efficiency, with accuracy superior to other diagnostic methods currently available for scabies. Due to the high sensitivity, specificity, accuracy, predictive values, and area under the ROC curve, the developed DELFIA represents a marked improvement for the clinical diagnosis of scabies and helps direct future development of a specific diagnostic tool for scabies. The study was published on October 21, 2011, in the journal Diagnostic Microbiology and Infectious Disease.
Related Links:
Menzies School of Health Research
Wallac
Latest Immunology News
- AI Predicts Tumor-Killing Cells with High Accuracy
- Diagnostic Blood Test for Cellular Rejection after Organ Transplant Could Replace Surgical Biopsies
- AI Tool Precisely Matches Cancer Drugs to Patients Using Information from Each Tumor Cell
- Genetic Testing Combined With Personalized Drug Screening On Tumor Samples to Revolutionize Cancer Treatment
- Testing Method Could Help More Patients Receive Right Cancer Treatment
- Groundbreaking Test Monitors Radiation Therapy Toxicity in Cancer Patients
- State-Of-The Art Techniques to Investigate Immune Response in Deadly Strep A Infections
- Novel Immunoassays Enable Early Diagnosis of Antiphospholipid Syndrome
- New Test Could Predict Immunotherapy Success for Broader Range Of Cancers
- Simple Blood Protein Tests Predict CAR T Outcomes for Lymphoma Patients
- Cell Sorter Chip Technology to Pave Way for Immune Profiling at POC
- Chip Monitors Cancer Cells in Blood Samples to Assess Treatment Effectiveness
- Automated Immunohematology Approaches Can Resolve Transplant Incompatibility
- AI Leverages Tumor Genetics to Predict Patient Response to Chemotherapy
- World’s First Portable, Non-Invasive WBC Monitoring Device to Eliminate Need for Blood Draw
- Predictive T-Cell Test Detects Immune Response to Viruses Even Before Antibodies Form