Assay Detects Urinary Proteins in Bladder Cancer
|
By LabMedica International staff writers Posted on 08 Jul 2013 |
|
Image: Synergy HT Multi-Mode microplate reader (Photo courtesy of Biotek).
A high-throughput assay has been developed for the detection of tyrosine-phosphorylated proteins (TPPs) in urine of bladder cancer patients.
Urinary TPPs appear to be a potential cancer marker for the detection of early stages of bladder cancer, but the development of methods to perform the evaluation of their diagnostic performances and their clinical validation is crucial.
Oncologists at the University of Turin Medical School (Turin, Italy) collected urine specimens from 23 bladder cancer patients and 43 healthy volunteers. There were 19 males and 4 females with bladder cancer with a mean age of 67.65 ± 10.63. The scientists developed an immobilized metal affinity chromatography (IMAC) system that was miniaturized in a 96 well format. Luminescence, visible, and infrared fluorescence antibody-based detection methods were comparatively evaluated.
The luminescence end-point detection was performed by means of a Synergy HT Multi-Mode microplate reader (Biotek; Winooski, VT, USA) and infrared (IR) fluorescence detection was performed with the Odyssey Infrared Imaging System (LI-COR Biosciences; Lincoln, NE, USA). Due to their low abundance, both a phosphoprotein enrichment step and very sensitive detection methods are required to detect TPPs in urine samples. To pursue high throughput, reproducibility and cost containment, which are required for bladder cancer screening programs. The scientists coupled the preanalytical IMAC procedure with high sensitive detection phases using either infrared fluorescence or chemiluminescence in an automated platform.
The assay represents the first quantitative and high throughput method for the measurement of TPPs in urine. Preliminary evaluation of the sensitivity and the specificity was 87% and 95% respectively, which confirmed the excellent diagnostic performances of TPPs as bladder cancer marker. The authors concluded that their method had optimized the measurement of TPP levels in urine samples, integrating the preanalytical and analytical phases in a 96 well format and with the use of affordable detection methods. This method is now feasible to be applied to a large number of patients and high-risk subjects to establish its actual diagnostic and predictive power. The study was published in the June 2013 issue of the journal Biochimica et Biophysica Acta.
Related Links:
University of Turin Medical School
Biotek
LI-COR Biosciences
Urinary TPPs appear to be a potential cancer marker for the detection of early stages of bladder cancer, but the development of methods to perform the evaluation of their diagnostic performances and their clinical validation is crucial.
Oncologists at the University of Turin Medical School (Turin, Italy) collected urine specimens from 23 bladder cancer patients and 43 healthy volunteers. There were 19 males and 4 females with bladder cancer with a mean age of 67.65 ± 10.63. The scientists developed an immobilized metal affinity chromatography (IMAC) system that was miniaturized in a 96 well format. Luminescence, visible, and infrared fluorescence antibody-based detection methods were comparatively evaluated.
The luminescence end-point detection was performed by means of a Synergy HT Multi-Mode microplate reader (Biotek; Winooski, VT, USA) and infrared (IR) fluorescence detection was performed with the Odyssey Infrared Imaging System (LI-COR Biosciences; Lincoln, NE, USA). Due to their low abundance, both a phosphoprotein enrichment step and very sensitive detection methods are required to detect TPPs in urine samples. To pursue high throughput, reproducibility and cost containment, which are required for bladder cancer screening programs. The scientists coupled the preanalytical IMAC procedure with high sensitive detection phases using either infrared fluorescence or chemiluminescence in an automated platform.
The assay represents the first quantitative and high throughput method for the measurement of TPPs in urine. Preliminary evaluation of the sensitivity and the specificity was 87% and 95% respectively, which confirmed the excellent diagnostic performances of TPPs as bladder cancer marker. The authors concluded that their method had optimized the measurement of TPP levels in urine samples, integrating the preanalytical and analytical phases in a 96 well format and with the use of affordable detection methods. This method is now feasible to be applied to a large number of patients and high-risk subjects to establish its actual diagnostic and predictive power. The study was published in the June 2013 issue of the journal Biochimica et Biophysica Acta.
Related Links:
University of Turin Medical School
Biotek
LI-COR Biosciences
Gold Member
Aspiration System
VACUSAFE
Steam Sterilizer
Hi Vac II Line
POC Immunoassay Analyzer
Procise DX
Latest Clinical Chem. News
- FDA-Approved Test Identifies Low Risk of Large Esophageal Varices in Cirrhosis
- Blood Protein Signature Diagnoses Pediatric IBD and Distinguishes Subtypes
- Blood Test Detects More High-Risk Prostate Cancers Than PSA
- Rapid Blood Test Aids Diagnosis of Acute Ischemic Stroke
- Blood-Based Alzheimer’s Testing Platform Offers Rapid Results
- Maternal Blood Biomarkers Identify Risk of Preterm and Early-Term Birth
- Simple Oral Swab Monitors Persistent Inflammation in Primary Ciliary Dyskinesia
- Simple Blood-Based Cholesterol Efflux Assay Identifies High-Risk Coronary Plaque Features
- Plasma Vitamin C Levels Associated with Brain Structure and Connectivity in Aging
- Mass Spectrometry Detects Tumor Metabolites for Cancer Monitoring
- Urinary Biomarker Assay Predicts Kidney Disease Progression Beyond Standard Measures
- Saliva-Based Test Detects Biochemical Signs of Sleep Loss
- Simple Dual-Tau Blood Test Detects and Stages Alzheimer’s Disease
- Alzheimer’s Blood Biomarkers Linked to Early Cognitive Differences Before Dementia
- Urine-Based Test Shows Promise for Autism Screening in Children
- Blood-Based Sensor Detects Early Signs of Alzheimer’s and Parkinson’s
Channels
Molecular Diagnostics
view channel
New Molecular Marker Helps Predict Multiple Myeloma Prognosis
Multiple myeloma is a bone marrow cancer marked by resistance to therapy and frequent relapse, complicating long-term disease control. Better molecular markers are needed to refine risk assessment and... Read more
Blood-Based RNA Biomarker Improves Prediction of Alzheimer’s Onset
Timely identification of patients approaching symptomatic Alzheimer’s disease (AD) remains a major clinical challenge, even as blood-based biomarkers continue to advance. Current assays are highly effective... Read more
Hematology
view channel
Next-Generation Hematology Platform Streamlines High-Complexity Lab Workflows
Sysmex America (Chicago, IL, USA) has introduced the next generation XR-Series, centered on the XR-10 Automated Hematology Module for high-complexity laboratories. The platform builds on the widely used... Read more
Blood Eosinophil Count May Predict Cancer Immunotherapy Response and Toxicity
Immune checkpoint inhibitors have improved outcomes across many cancers, yet only a subset of patients derive durable benefit and biomarkers to guide treatment remain limited. Eosinophils, best known for... Read more
Immunology
view channel
Anti-Lipid Antibody Biomarkers May Identify Early Lyme Disease and Persistent Symptoms
Lyme disease is often missed during its earliest and most treatable stage, while current serologic assays cannot distinguish active infection from prior exposure. Nearly half a million Americans are diagnosed... Read more
Emergency Department Opt-Out Testing Program Identifies Undiagnosed HIV
Undiagnosed HIV continues to drive avoidable morbidity and transmission, with many people identified only after substantial immune damage has occurred. In England, about one in 20 people living with HIV... Read more
Immune Biomarkers Could Identify Risk of Chronic Critical Illness on ICU Admission
Severe traumatic injury can trigger immune and organ dysfunction that complicates recovery in the intensive care unit. A subset of patients develop chronic critical illness, defined as dependence on intensive... Read more
Microbiology
view channel
Rapid Gastrointestinal PCR Panels Deliver One-Hour Results
Acute infectious gastroenteritis remains a major cause of illness worldwide, especially in young children, older adults, and immunocompromised patients. Nonspecific symptoms such as diarrhea, vomiting,... Read more
H. pylori Screening Within Colorectal Program Aids Gastric Cancer Prevention
Health systems increasingly rely on economic evidence to guide cancer prevention strategies. For gastric cancer, selecting screening approaches that can integrate with existing programs is a key policy question.... Read more
Machine Learning Reveals Consistent Gut Microbiome Patterns in Colorectal Cancer
Colorectal cancer has been repeatedly linked to alterations in the gut microbiome, yet findings have often varied across small, heterogeneous studies. Reproducibility has been limited by differing sequencing... Read more
Pathology
view channel
AI Pathology Tool Predicts Immunotherapy Response in Rare Cancers
Immunotherapy has transformed care for select malignancies, yet predicting which patients with rare cancers are most likely to benefit remains challenging. Clinicians often have only limited biomarkers... Read more
Uncertainty-Aware AI Tool Improves Digital Pathology for Cancer Subtyping
Reliable histologic subtyping guides therapy selection in oncology, yet diagnostic workflows grow more complex as whole-slide imaging and artificial intelligence (AI) expand. A persistent obstacle to clinical... Read more
Technology
view channel
AI Platform Links Biomarker Results to Cancer Clinical Trials and Guidelines
Oncology teams must manage growing volumes of genomic data, rapidly evolving clinical trial options, and frequently updated care guidelines, all within tight clinic schedules. Translating complex tumor... Read more
Agentic AI Platform Supports Genomic Decision-Making in Oncology
Oncology care teams increasingly face the challenge of managing complex molecular diagnostics, evolving treatment options, and extensive electronic health record documentation. Translating multimodal data... Read more
Industry
view channel
Partnership Integrates Automated DNA Extraction with Single-Molecule Digital PCR
Countable Labs (Palo Alto, CA, USA) and Promega (Madison, WI, USA) have entered a co-marketing agreement that integrates the Promega Maxwell System for nucleic acid extraction with Countable Labs’ Countable... Read more
