Lasers Used to Build Scaffolds to Help in Neuron Regeneration

Lasers are being used to construct scaffolds to be used as delivery vehicles to drop cells off at damaged locations and help treat diseases such as Alzheimer’s and Parkinson’s.

Because of the versatility and accuracy of lasers, the structures have shown significant potential for facilitating the growth of cells, and they could be designed specifically to meet the individual needs of a variety of cells.

In their study published September 20, 2011, in IOP Publishing’s journal Biofabrication, researchers employed lasers to create intricate scaffolds, with micrometer-sized features, and demonstrated their ability to harness effectively the growth of neuronal cells. The repairing of neural tissue--such as peripheral nerves, spinal cord, and the brain--has long been studied using a technique known as tissue engineering and is now becoming a realistic treatment as technology advances.

A multidisciplinary field in which tissues and organs are formed by growing cells onto materials outside of the body, tissue engineering relies heavily on the creation of scaffolds that determine the efficiency, size, shape, and orientation of cell growth. By modifying the composition and design of these scaffolds, they could be utilized as a transport vehicle to drop cells off at a specific damaged site, help them attach and grow, and then become degraded in the body without any damage to the cells or the host.

In this study, the researchers from the University of Crete (Rethymno and Heraklio, Greece) and the University of Sheffield (UK), constructed a scaffold from a commonly used polymer, polylactic acid (PLA). This synthetic, biocompatible substance degrades in the human body to form lactic acid, a naturally occurring chemical that can easily be removed, leaving the regenerated tissue behind in the required size, shape, and structure.

An ultra-fast, titanium sapphire laser was tightly focused on the PLA material and moved through three dimensions (3D) to create complicated sub-micrometer structures. Within the structures, small struts and holes were fabricated to ensure stability and the efficient delivery of nutrients to the cells; both are essential for tissue generation.

Taking the fabrication one-step further, 3D seashell structures were created from the polymer to demonstrate the intricacy of the laser technique. To evaluate the compatibility of the structures, the researchers grew neuronal cells on them and observed, using high-powered microscopes, how the cells proliferated and aligned. The neuronal cells showed good compatibility with the PLA structures with less than 10% of the cells dying after five days.

Study coauthor, Prof. Frederik Claeyssens, said, “This is the first time we have been able to structure polylactide with such high resolution and the first time that direct laser writing has been applied to tissue engineering. “Compared to other techniques, direct laser writing allows the scaffold to be created in a user-defined manner on the micrometer level and provides the possibility to explore the relationship between structure of, and cell growth on, the scaffold.”

An Institute of Physics spokesperson said, “The fabrication of appropriate scaffolds is a vital step in the process of tissue engineering and must be fine-tuned if treatments for Alzheimer’s and Parkinson’s are to be realized.”

Related Links:
University of Crete
University of Sheffield