Control DNA Panels for PCR Studies

A panel of control DNA samples for polymerase chain reaction (PCR) studies has been established to aid researchers working on gene regulation.

Sigma, a division of Sigma-Aldrich Corporation (St. Louis, MO, USA), and the European Collection of Cell Cultures (ECACC) (Porton Down, UK) have established the human random control (HRC) DNA collection obtained from 480 UK Caucasian blood donors.

The DNA samples are maintained in lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) that can be continuously propagated in culture. All samples are completely defined and standardized so that they may be used as reference standards for research or quality control.

The purified DNA is available in five different panels. Each panel contains 10 micrograms of DNA at a concentration of 200 nanograms/microliter and represents 96 individuals. For convenience, three of the panels are available in 96-well format for use in PCR without the need for aliquoting or dilution at a concentration of 8 nanograms/microliter.

"The ECACC HRC control panels are an excellent complement to Sigma's Quantitative PCR product offering,” said Keith Jolliff, global marketing manager of molecular biology at Sigma-Aldrich. "The DNA panels are unique products that offer researchers convenience and quality control for gene regulation and quantification studies.”



Related Links:
Sigma-Aldrich Corporation
European Collection of Cell Cultures