New Fluorescence Assays for Molecular Biology
By Biotechdaily staff writers
Posted on 01 Jul 2004
Several new products for the quantitation of nucleic acids and proteins are now available to researchers in the field of molecular biology. These include separate, ready-for-use kits for measuring DNA, RNA, and protein.Posted on 01 Jul 2004
The new fluorescence assays comprise the Quant-iTT line marked by Molecular Probes (Eugene, OR USA). Separate kits are available for DNA, RNA, and protein. The fluorescent dye in each Quant-iT kit matches common fluorescence excitation and emission filter sets in fluorescent microplate readers.
The DNA kit is described as being several orders of magnitude more sensitive than UV absorbance methods while requiring only one to 20 µl of sample. The wide assay range eliminates the need to dilute concentrated samples.
The RNA kit is cited as being the first homogenous assay ever developed for quantitation of RNA in the presence of DNA. Molecular Probes says that the protein assay is easier to perform and more sensitive than standard absorbance based assays. The signal is unaffected by many common contaminants including DTT, beta-mercaptoethanol, amino acids, and DNA.
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