Tumor Microbiome Appears to Vary with Cancer Type
By LabMedica International staff writers Posted on 11 Jun 2020 |
Image: Bacteria (green) inside human pancreatic cancer cells (AsPC-1 cells). The cells’ nuclei are stained blue while their cytoplasm is stained orange (Photo courtesy of Weizmann Institute of Science).
Bacteria are well-known residents in human tumors, but whether their presence is advantageous to the tumors or to the bacteria themselves has been unclear. Bacteria found within tumors were localized within both cancer cells and immune cells and that the bacterial composition varied according to tumor type.
In what outside experts called the most rigorous and comprehensive survey of bacteria in human tumor samples, scientists have discovered distinct populations of microbes living inside different types of tumors: In other words, breast and brain and pancreatic tumors have their own characteristic microbiomes.
A large international team of scientists working with the Weizmann Institute of Science (Rehovot, Israel) undertook a comprehensive analysis of the tumor microbiome, studying 1,526 tumors and their adjacent normal tissues across seven cancer types, including breast, lung, ovary, pancreas, melanoma, bone, and brain tumors. The team utilized 16S ribosomal gene sequencing and quantitative polymerase chain reaction (PCR) to identify and quantify bacteria present in 1,010 tumor samples and more than 500 neighboring normal tissue samples.
The samples were collected from patients at nine medical centers, and the scientists focused on 528 bacterial species found in the tumors in their analysis. The team used immunohistochemistry, fluorescence in situ hybridization with 16S rRNA-targeting probes, and other pathology approaches and they showed that the majority of the microbes identified in the tumor samples were intracellular bacteria nestled within tumor and immune cells. This finding was supported by follow-up testing on four freshly collected breast tumor samples.
The investigators reported that each tumor type has a distinct microbiome composition and that breast cancer has a particularly rich and diverse microbiome. The intratumor bacteria are mostly intracellular and are present in both cancer and immune cells. They also noted correlations between intratumor bacteria or their predicted functions with tumor types and subtypes, patients’ smoking status, and the response to immunotherapy. The study was published on May 29, 2020 in the journal Science.
Related Links:
Weizmann Institute of Science
In what outside experts called the most rigorous and comprehensive survey of bacteria in human tumor samples, scientists have discovered distinct populations of microbes living inside different types of tumors: In other words, breast and brain and pancreatic tumors have their own characteristic microbiomes.
A large international team of scientists working with the Weizmann Institute of Science (Rehovot, Israel) undertook a comprehensive analysis of the tumor microbiome, studying 1,526 tumors and their adjacent normal tissues across seven cancer types, including breast, lung, ovary, pancreas, melanoma, bone, and brain tumors. The team utilized 16S ribosomal gene sequencing and quantitative polymerase chain reaction (PCR) to identify and quantify bacteria present in 1,010 tumor samples and more than 500 neighboring normal tissue samples.
The samples were collected from patients at nine medical centers, and the scientists focused on 528 bacterial species found in the tumors in their analysis. The team used immunohistochemistry, fluorescence in situ hybridization with 16S rRNA-targeting probes, and other pathology approaches and they showed that the majority of the microbes identified in the tumor samples were intracellular bacteria nestled within tumor and immune cells. This finding was supported by follow-up testing on four freshly collected breast tumor samples.
The investigators reported that each tumor type has a distinct microbiome composition and that breast cancer has a particularly rich and diverse microbiome. The intratumor bacteria are mostly intracellular and are present in both cancer and immune cells. They also noted correlations between intratumor bacteria or their predicted functions with tumor types and subtypes, patients’ smoking status, and the response to immunotherapy. The study was published on May 29, 2020 in the journal Science.
Related Links:
Weizmann Institute of Science
Latest Microbiology News
- Clinical Decision Support Software a Game-Changer in Antimicrobial Resistance Battle
- New CE-Marked Hepatitis Assays to Help Diagnose Infections Earlier
- 1 Hour, Direct-From-Blood Multiplex PCR Test Identifies 95% of Sepsis-Causing Pathogens
- Mouth Bacteria Test Could Predict Colon Cancer Progression
- Unique Metabolic Signature Could Enable Sepsis Diagnosis within One Hour of Blood Collection
- Groundbreaking Diagnostic Platform Provides AST Results With Unprecedented Speed
- Simple Blood Test Combined With Personalized Risk Model Improves Sepsis Diagnosis
- Blood Analysis Predicts Sepsis and Organ Failure in Children
- TB Blood Test Could Detect Millions of Silent Spreaders
- New Blood Test Cuts Diagnosis Time for Nontuberculous Mycobacteria Infections from Months to Hours
- New Tuberculosis Test to Expand Testing Access in Low- and Middle-Income Countries
- Rapid Test Diagnoses Tropical Disease within Hours for Faster Antibiotics Treatment
- Rapid Molecular Testing Enables Faster, More Targeted Antibiotic Treatment for Pneumonia
- Rapid AST Platform Provides Targeted Therapeutic Results Days Faster Than Current Standard of Care
- New Analysis Method Detects Pathogens in Blood Faster and More Accurately by Melting DNA
- Rapid Sepsis Test Delivers Two Days Faster Results