Genetic Defect Linked to Pediatric Liver Disease
By LabMedica International staff writers Posted on 04 Mar 2019 |
Image: A histopathology of the bile duct of a patient with biliary atresia: loss of bile ducts, brisk ductular reaction, and bile plugs (Photo courtesy of Hopkins GI Pathology).
Biliary atresia (BA) is the most common cause of end‐stage liver disease in children and the primary indication for pediatric liver transplantation, yet underlying etiologies remain unknown.
Approximately 10% of infants affected by BA exhibit various laterality defects (heterotaxy) including splenic abnormalities and complex cardiac malformations, a distinctive subgroup commonly referred to as the biliary atresia splenic malformation (BASM) syndrome.
A large team of medical scientists at the University of Colorado Anschutz (Aurora, CO, USA) sequenced DNA specimens from 67 subjects with BASM, including 58 patient-parent trios. They looked at 2,016 genes, a subset of the full genome that was associated with proteins that were candidates to cause a disease like BA. Candidate gene variants derived from the pre‐specified set of 2,016 genes associated with ciliary dysgenesis and/or dysfunction or cholestasis were prioritized according to pathogenicity, population frequency, and mode of inheritance.
The team found five BASM subjects harbored rare and potentially deleterious bi‐allelic variants in polycystin 1‐like 1, (PKD1L1), a gene associated with ciliary calcium signaling and embryonic laterality determination. Heterozygous PKD1L1 variants were found in three additional subjects. Immunohistochemical analysis of liver from the one BASM subject available revealed decreased PKD1L1 expression in bile duct epithelium when compared to normal livers and livers affected by other non‐cholestatic diseases.
Ronald Sokol, MD, a pediatric gastroenterologist and co-author of the study, said, “We don't know the cause of biliary atresia, which interferes with our ability to treat affected children. The importance of this is that scientists have never identified a gene, when mutated that causes BA. This is the first time it has been found.”
The authors concluded that whole exome sequencing identified bi‐allelic and heterozygous PKD1L1 variants of interest in eight BASM subjects from the ChiLDReN dataset. The dual roles for PKD1L1 in laterality determination and ciliary function suggest that PKD1L1 is a new, biologically plausible, cholangiocyte‐expressed candidate gene for the BASM syndrome. The study was published on January 21, 2019, in the journal Hepatology.
Related Links:
University of Colorado Anschutz
Approximately 10% of infants affected by BA exhibit various laterality defects (heterotaxy) including splenic abnormalities and complex cardiac malformations, a distinctive subgroup commonly referred to as the biliary atresia splenic malformation (BASM) syndrome.
A large team of medical scientists at the University of Colorado Anschutz (Aurora, CO, USA) sequenced DNA specimens from 67 subjects with BASM, including 58 patient-parent trios. They looked at 2,016 genes, a subset of the full genome that was associated with proteins that were candidates to cause a disease like BA. Candidate gene variants derived from the pre‐specified set of 2,016 genes associated with ciliary dysgenesis and/or dysfunction or cholestasis were prioritized according to pathogenicity, population frequency, and mode of inheritance.
The team found five BASM subjects harbored rare and potentially deleterious bi‐allelic variants in polycystin 1‐like 1, (PKD1L1), a gene associated with ciliary calcium signaling and embryonic laterality determination. Heterozygous PKD1L1 variants were found in three additional subjects. Immunohistochemical analysis of liver from the one BASM subject available revealed decreased PKD1L1 expression in bile duct epithelium when compared to normal livers and livers affected by other non‐cholestatic diseases.
Ronald Sokol, MD, a pediatric gastroenterologist and co-author of the study, said, “We don't know the cause of biliary atresia, which interferes with our ability to treat affected children. The importance of this is that scientists have never identified a gene, when mutated that causes BA. This is the first time it has been found.”
The authors concluded that whole exome sequencing identified bi‐allelic and heterozygous PKD1L1 variants of interest in eight BASM subjects from the ChiLDReN dataset. The dual roles for PKD1L1 in laterality determination and ciliary function suggest that PKD1L1 is a new, biologically plausible, cholangiocyte‐expressed candidate gene for the BASM syndrome. The study was published on January 21, 2019, in the journal Hepatology.
Related Links:
University of Colorado Anschutz
Latest Pathology News
- AI Integrated With Optical Imaging Technology Enables Rapid Intraoperative Diagnosis
- HPV Self-Collection Solution Improves Access to Cervical Cancer Testing
- Hyperspectral Dark-Field Microscopy Enables Rapid and Accurate Identification of Cancerous Tissues
- AI Advancements Enable Leap into 3D Pathology
- New Blood Test Device Modeled on Leeches to Help Diagnose Malaria
- Robotic Blood Drawing Device to Revolutionize Sample Collection for Diagnostic Testing
- Use of DICOM Images for Pathology Diagnostics Marks Significant Step towards Standardization
- First of Its Kind Universal Tool to Revolutionize Sample Collection for Diagnostic Tests
- AI-Powered Digital Imaging System to Revolutionize Cancer Diagnosis
- New Mycobacterium Tuberculosis Panel to Support Real-Time Surveillance and Combat Antimicrobial Resistance
- New Method Offers Sustainable Approach to Universal Metabolic Cancer Diagnosis
- Spatial Tissue Analysis Identifies Patterns Associated With Ovarian Cancer Relapse
- Unique Hand-Warming Technology Supports High-Quality Fingertip Blood Sample Collection
- Image-Based AI Shows Promise for Parasite Detection in Digitized Stool Samples
- Deep Learning Powered AI Algorithms Improve Skin Cancer Diagnostic Accuracy
- Microfluidic Device for Cancer Detection Precisely Separates Tumor Entities