Nanoscale Biosensor for Detection of Pathogenic Bacteria under Field Conditions
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By LabMedica International staff writers Posted on 27 Jun 2016 |
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Image: A colorized scanning electron micrograph (SEM) showing a number of Gram-negative Escherichia coli bacteria of the strain O157:H7 (Photo courtesy of the CDC).
A team of bioengineers used nanoscale technology to develop a simple, portable biosensor system for detection of pathogenic bacteria – such as the diarrhea-causing Escherichia coli (E. coli) 0157:H7 – under field conditions.
Investigators at Washington State University (Pullman, USA) used organic and inorganic components to create nanoparticles, which resembled miniature flowers under the microscope. These "nanoflowers" provided a large surface area for immobilizing the enzymes that needed to detect low levels of the bacteria. Binding of the specific target bacteria triggered a change in acidity that was amplified to be measurable on a handheld pH meter or with a pH indicator paper strip.
“We want to take these nanoflowers and create a simple-to-use, handheld device that anyone can use anywhere,” said senior author Dr. Yuehe Lin, professor of mechanical and materials engineering at Washington State University. “It will be as simple as using a pregnancy test strip or a glucose meter.”
A patent application has been filed for the methodology, which was also described in the June 15, 2016, issue of the journal Small.
Related Links:
Washington State University
Investigators at Washington State University (Pullman, USA) used organic and inorganic components to create nanoparticles, which resembled miniature flowers under the microscope. These "nanoflowers" provided a large surface area for immobilizing the enzymes that needed to detect low levels of the bacteria. Binding of the specific target bacteria triggered a change in acidity that was amplified to be measurable on a handheld pH meter or with a pH indicator paper strip.
“We want to take these nanoflowers and create a simple-to-use, handheld device that anyone can use anywhere,” said senior author Dr. Yuehe Lin, professor of mechanical and materials engineering at Washington State University. “It will be as simple as using a pregnancy test strip or a glucose meter.”
A patent application has been filed for the methodology, which was also described in the June 15, 2016, issue of the journal Small.
Related Links:
Washington State University
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