Blood Test Simplifies Diagnosis of Celiac Disease
|
By LabMedica International staff writers Posted on 29 Jan 2014 |
|
Image: AID ELISPOT reader system (Photo courtesy of Autoimmun Diagnostika GmbH).
A new blood test is being developed that can rapidly and accurately diagnose celiac disease without the need for prolonged gluten exposure.
The diagnostic test gave a result within 24 hours and preliminary findings indicated it could accurately detect celiac disease and it is hoped that larger studies will verify its role as a widely used tool for diagnosing celiac disease (CD).
Scientists at the Walter and Eliza Hall Institute (Melbourne, Australia) recruited 27 patients being treated for CD, four with CD but were untreated and 17 non-CD controls. Blood for serology and cytokine release assays was drawn in the morning immediately before (d0) and on day six (d6) after commencing gluten challenge, or prior to commencing a gluten-free diet (GFD) in untreated CD patients.
Serum transglutaminase (tTG-IgA), deamidated gliadin peptide (DGP)-immunoglobulin (Ig)A and DGP-IgG were evaluated with commercial kits (INOVA Diagnostics; San Diego, CA, USA). Plasma interferon gamma (IFN-γ) levels on d0 and d6 were measured in triplicate by enzyme-linked immunosorbent assay (ELISA) and measured on an automated ELISA reader. The investigators also performed the IFN-γ ELISPOT assay and spot-forming units (SFU) in individual wells were counted using an automated AID ELISPOT reader system (Autoimmun Diagnostika GmbH; Strasberg, Germany) and results expressed as SFU per 106 peripheral blood mononuclear cells (PBMC).
The authors concluded that the whole blood cytokine release assays appear to be sensitive and specific potential diagnostic test for CD patients following GFD. As an added benefit over current diagnostic tests being performed on patients already following GFD, the mobilization of gluten-reactive T-cells specific for CD into the bloodstream requires oral gluten challenge for only three days instead of the weeks or months required for diagnosis based on abnormal small bowel histology.
Jason Tye-Din, MBBS, PhD, FRACP, a gastroenterologist and a coauthor of the study said, “A test that simplifies diagnosis for patients is likely to significantly enhance disease detection. This new diagnostic approach is encouraging and we hope that larger studies can validate these findings and establish its role in the diagnosis of celiac disease, with the possibility of avoiding intestinal biopsies for diagnosis altogether.” The study was published on January 3, 2014, in the journal Clinical & Experimental Immunology.
Related Links:
Walter and Eliza Hall Institute
INOVA Diagnostics
Autoimmun Diagnostika GmbH
The diagnostic test gave a result within 24 hours and preliminary findings indicated it could accurately detect celiac disease and it is hoped that larger studies will verify its role as a widely used tool for diagnosing celiac disease (CD).
Scientists at the Walter and Eliza Hall Institute (Melbourne, Australia) recruited 27 patients being treated for CD, four with CD but were untreated and 17 non-CD controls. Blood for serology and cytokine release assays was drawn in the morning immediately before (d0) and on day six (d6) after commencing gluten challenge, or prior to commencing a gluten-free diet (GFD) in untreated CD patients.
Serum transglutaminase (tTG-IgA), deamidated gliadin peptide (DGP)-immunoglobulin (Ig)A and DGP-IgG were evaluated with commercial kits (INOVA Diagnostics; San Diego, CA, USA). Plasma interferon gamma (IFN-γ) levels on d0 and d6 were measured in triplicate by enzyme-linked immunosorbent assay (ELISA) and measured on an automated ELISA reader. The investigators also performed the IFN-γ ELISPOT assay and spot-forming units (SFU) in individual wells were counted using an automated AID ELISPOT reader system (Autoimmun Diagnostika GmbH; Strasberg, Germany) and results expressed as SFU per 106 peripheral blood mononuclear cells (PBMC).
The authors concluded that the whole blood cytokine release assays appear to be sensitive and specific potential diagnostic test for CD patients following GFD. As an added benefit over current diagnostic tests being performed on patients already following GFD, the mobilization of gluten-reactive T-cells specific for CD into the bloodstream requires oral gluten challenge for only three days instead of the weeks or months required for diagnosis based on abnormal small bowel histology.
Jason Tye-Din, MBBS, PhD, FRACP, a gastroenterologist and a coauthor of the study said, “A test that simplifies diagnosis for patients is likely to significantly enhance disease detection. This new diagnostic approach is encouraging and we hope that larger studies can validate these findings and establish its role in the diagnosis of celiac disease, with the possibility of avoiding intestinal biopsies for diagnosis altogether.” The study was published on January 3, 2014, in the journal Clinical & Experimental Immunology.
Related Links:
Walter and Eliza Hall Institute
INOVA Diagnostics
Autoimmun Diagnostika GmbH
Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Anti-Cyclic Citrullinated Peptide Test
GPP-100 Anti-CCP Kit
New
Gold Member
TORCH Panel Rapid Test
Rapid TORCH Panel Test
Latest Immunology News
- AI Predicts Tumor-Killing Cells with High Accuracy
- Diagnostic Blood Test for Cellular Rejection after Organ Transplant Could Replace Surgical Biopsies
- AI Tool Precisely Matches Cancer Drugs to Patients Using Information from Each Tumor Cell
- Genetic Testing Combined With Personalized Drug Screening On Tumor Samples to Revolutionize Cancer Treatment
- Testing Method Could Help More Patients Receive Right Cancer Treatment
- Groundbreaking Test Monitors Radiation Therapy Toxicity in Cancer Patients
- State-Of-The Art Techniques to Investigate Immune Response in Deadly Strep A Infections
- Novel Immunoassays Enable Early Diagnosis of Antiphospholipid Syndrome
- New Test Could Predict Immunotherapy Success for Broader Range Of Cancers
- Simple Blood Protein Tests Predict CAR T Outcomes for Lymphoma Patients
- Cell Sorter Chip Technology to Pave Way for Immune Profiling at POC
- Chip Monitors Cancer Cells in Blood Samples to Assess Treatment Effectiveness
- Automated Immunohematology Approaches Can Resolve Transplant Incompatibility
- AI Leverages Tumor Genetics to Predict Patient Response to Chemotherapy
- World’s First Portable, Non-Invasive WBC Monitoring Device to Eliminate Need for Blood Draw
- Predictive T-Cell Test Detects Immune Response to Viruses Even Before Antibodies Form
Channels
Clinical Chemistry
view channel
3D Printed Point-Of-Care Mass Spectrometer Outperforms State-Of-The-Art Models
Mass spectrometry is a precise technique for identifying the chemical components of a sample and has significant potential for monitoring chronic illness health states, such as measuring hormone levels... Read more.jpg "Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)")
POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection
Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more
Highly Reliable Cell-Based Assay Enables Accurate Diagnosis of Endocrine Diseases
The conventional methods for measuring free cortisol, the body's stress hormone, from blood or saliva are quite demanding and require sample processing. The most common method, therefore, involves collecting... Read more
Molecular Diagnostics
view channel
Blood Proteins Could Warn of Cancer Seven Years before Diagnosis
Two studies have identified proteins in the blood that could potentially alert individuals to the presence of cancer more than seven years before the disease is clinically diagnosed. Researchers found... Read more (1)_1.jpg "Image: Ultrasound image of micro-histotripsy liberation of biomarkers in a tumor (Photo courtesy of Joy Wang and Pradyumna Kedarisetti)"){kind=small}
Ultrasound-Aided Blood Testing Detects Cancer Biomarkers from Cells
Ultrasound imaging serves as a noninvasive method to locate and monitor cancerous tumors effectively. However, crucial details about the cancer, such as the specific types of cells and genetic mutations... Read more_1.jpg "Image: The innovative method to customize and strengthen DNA origami structure promises breakthroughs in medicine (Photo courtesy of Shutterstock)")
.jpg "Image: The QIAstat-Dx Respiratory Panel Plus has received U.S. FDA clearance (Photo courtesy of QIAGEN)")
Hematology
view channel
Next Generation Instrument Screens for Hemoglobin Disorders in Newborns
Hemoglobinopathies, the most widespread inherited conditions globally, affect about 7% of the population as carriers, with 2.7% of newborns being born with these conditions. The spectrum of clinical manifestations... Read more
First 4-in-1 Nucleic Acid Test for Arbovirus Screening to Reduce Risk of Transfusion-Transmitted Infections
Arboviruses represent an emerging global health threat, exacerbated by climate change and increased international travel that is facilitating their spread across new regions. Chikungunya, dengue, West... Read more
POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy
Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more
First Affordable and Rapid Test for Beta Thalassemia Demonstrates 99% Diagnostic Accuracy
Hemoglobin disorders rank as some of the most prevalent monogenic diseases globally. Among various hemoglobin disorders, beta thalassemia, a hereditary blood disorder, affects about 1.5% of the world's... Read moreMicrobiology
view channel
Integrated Solution Ushers New Era of Automated Tuberculosis Testing
Tuberculosis (TB) is responsible for 1.3 million deaths every year, positioning it as one of the top killers globally due to a single infectious agent. In 2022, around 10.6 million people were diagnosed... Read more
Automated Sepsis Test System Enables Rapid Diagnosis for Patients with Severe Bloodstream Infections
Sepsis affects up to 50 million people globally each year, with bacteraemia, formerly known as blood poisoning, being a major cause. In the United States alone, approximately two million individuals are... Read more
Enhanced Rapid Syndromic Molecular Diagnostic Solution Detects Broad Range of Infectious Diseases
GenMark Diagnostics (Carlsbad, CA, USA), a member of the Roche Group (Basel, Switzerland), has rebranded its ePlex® system as the cobas eplex system. This rebranding under the globally renowned cobas name... Read more
Clinical Decision Support Software a Game-Changer in Antimicrobial Resistance Battle
Antimicrobial resistance (AMR) is a serious global public health concern that claims millions of lives every year. It primarily results from the inappropriate and excessive use of antibiotics, which reduces... Read morePathology
view channel
AI Integrated With Optical Imaging Technology Enables Rapid Intraoperative Diagnosis
Rapid and accurate intraoperative diagnosis is essential for tumor surgery as it guides surgical decisions with precision. Traditional intraoperative assessments, such as frozen sections based on H&E... Read more
HPV Self-Collection Solution Improves Access to Cervical Cancer Testing
Annually, over 604,000 women across the world are diagnosed with cervical cancer, and about 342,000 die from this disease, which is preventable and primarily caused by the Human Papillomavirus (HPV).... Read more
Hyperspectral Dark-Field Microscopy Enables Rapid and Accurate Identification of Cancerous Tissues
Breast cancer remains a major cause of cancer-related mortality among women. Breast-conserving surgery (BCS), also known as lumpectomy, is the removal of the cancerous lump and a small margin of surrounding tissue.... Read more
Technology
view channel
New Diagnostic System Achieves PCR Testing Accuracy
While PCR tests are the gold standard of accuracy for virology testing, they come with limitations such as complexity, the need for skilled lab operators, and longer result times. They also require complex... Read more
DNA Biosensor Enables Early Diagnosis of Cervical Cancer
Molybdenum disulfide (MoS2), recognized for its potential to form two-dimensional nanosheets like graphene, is a material that's increasingly catching the eye of the scientific community.... Read more
Self-Heating Microfluidic Devices Can Detect Diseases in Tiny Blood or Fluid Samples
Microfluidics, which are miniature devices that control the flow of liquids and facilitate chemical reactions, play a key role in disease detection from small samples of blood or other fluids.... Read more
Breakthrough in Diagnostic Technology Could Make On-The-Spot Testing Widely Accessible
Home testing gained significant importance during the COVID-19 pandemic, yet the availability of rapid tests is limited, and most of them can only drive one liquid across the strip, leading to continued... Read moreIndustry
view channel
Danaher and Johns Hopkins University Collaborate to Improve Neurological Diagnosis
Unlike severe traumatic brain injury (TBI), mild TBI often does not show clear correlations with abnormalities detected through head computed tomography (CT) scans. Consequently, there is a pressing need... Read more
Beckman Coulter and MeMed Expand Host Immune Response Diagnostics Partnership
Beckman Coulter Diagnostics (Brea, CA, USA) and MeMed BV (Haifa, Israel) have expanded their host immune response diagnostics partnership. Beckman Coulter is now an authorized distributor of the MeMed... Read more_1.jpg "Image: Thermo Fisher will distribute Bio-Techne products across Europe (Photo courtesy of 123RF)")
