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Molecular Diagnostics

Image: The Specificity of Recombinase Polymerase Amplification -Lateral Flow (RPA-LF) immunochromatographic strip to amplify species of the Viannia subgenus. The most relevant L. Viannia species (L. braziliensis, L. guyanensis, and L. panamensis) produced stronger bands in the lateral flow strip than other less common species of this subgenus. NTC= No template control (Photo courtesy of University of Texas Medical Branch).

Field-Applicable Molecular Test Diagnoses Tegumentary Leishmaniasis

Cutaneous and mucosal leishmaniases are widely distributed in Central and South America. Leishmania of the Viannia subgenus are the most frequent species infecting humans. L. (V.) braziliensis, L. (V.) panamensis are also responsible for metastatic mucosal leishmaniasis. More...
11 May 2016

Bacterial Vaginosis Assessed By Molecular Methods

Bacterial vaginosis (BV) is an aberrant state of the vaginal microbiota, which is characterized by a depletion of lactobacilli, an increased diversity of the bacterial population and an elevated pH. It is one the most common vaginal syndromes in fertile, premenopausal and pregnant women. More...
11 May 2016
Image: The fully automated nCounter analysis system (Photo courtesy of NanoString Technologies).

Novel Multiplexed Digital Immunohistochemistry Used On Tissue Sections

Intratumoral heterogeneity has emerged as a critical challenge to the implementation of targeted therapeutics and immunohistochemistry (IHC) has been used to assess spatial heterogeneity of proteins; however, it has been difficult to quantify protein abundance at high multiplex and wide dynamic range. More...
10 May 2016
Image: Predicted alteration in untranslated region (UTR) structure using mFOLD for variants flagged by SNPfold. Wild-type (a,c,e) and variant (b,d,f) structure pairs are displayed, with the variant location indicated by a red arrow. SHAPE analysis revealed differences in reactivity between mutant and variant mRNAs, confirming alterations to 2° structure (Image courtesy of Mucaki EJ et al, 2016, BMC Medical Genomics.)

New Strategy Broadens Detection of Breast & Ovarian Cancer Gene Variants

Using next generation sequencing (NGS) technology and information theory (IT), researchers have developed a new method for identifying mutations and prioritizing sequence variants related to hereditary breast and ovarian cancer (HBOC). This could not only reduce the number of possible variants to test for, but also increase the number of patients that are properly diagnosed. More...
09 May 2016

Liver Cancer May Be Diagnosed from Altered Sugar Metabolism

Altered gene expression that causes cancerous cells to metabolize fructose differently from healthy cells could be a significant new biomarker for diagnosing liver cancer. More...
04 May 2016
Image: The MammaPrint DNA microarray test for breast cancer prognosis (Photo courtesy of Agendia).

Genetic Test Reduces Post-Surgery Chemotherapy for Breast Cancer Patients

A genetic test identified a large group of patients for whom five-year distant metastasis-free survival was equally good whether or not they received adjuvant chemotherapy, chemotherapy given post-surgery. More...
04 May 2016
Image: The Cologuard testing kit for colorectal cancer (Photo courtesy of Exact Sciences).

Noninvasive Test Detects Colorectal Cancer in Unscreened Patients

A noninvasive colorectal cancer-screening test detected the disease in patients who had previously avoided more invasive screening measures. Colorectal cancer is the second deadliest form of cancer in the USA and this year, nearly 135,000 Americans will be diagnosed with the disease and 50,000 Americans will die of it. More...
03 May 2016
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Genetic Testing channel of LabMedica brings the latest in molecular genetics, cytogenetics, and epigenetics, and methods from PCR to FISH, and more.