LabMedica

Download Mobile App
Recent News Expo Clinical Chem. Molecular Diagnostics Hematology Immunology Microbiology Pathology Technology Industry Focus

Routine Light Transmission Platelet Aggregation Automated

By LabMedica International staff writers
Posted on 05 Aug 2014
Print article
The Sysmex CS-2000i fully automated blood coagulation analyzer
The CS-2000i fully automated blood coagulation analyzer (Photo courtesy of Sysmex America, Inc.)
Platelet aggregation is most commonly measured by light transmission aggregometry (LTA), in which the increase in light transmission through a stirred suspension of platelet-rich plasma (PRP) is monitored as platelets aggregate.

The assessment of platelet aggregation to a range of agonists including adenosine diphosphate (ADP), epinephrine, collagen, arachidonic acid and ristocetin is central to the investigation of platelet function disorders, but is only undertaken by a few specialized hemostasis laboratories.

Hematologists at the University College London (UK) working with colleagues from Japan and France, obtained blood samples from 14 normal healthy subjects not receiving any medication or who were self-medicating with nonsteroidal anti-inflammatory drugs, and from 2 individuals who were taking clopidogrel, an antiplatelet agent used to inhibit blood clots. Platelet aggregation was performed by examining the effect of varying reaction cuvette stirrer speed and the platelet count in PRP using the following agonists: ADP, epinephrine, collagen, ristocetin and arachidonic acid. Platelet aggregation reactions were measured on an AggRAM aggregometer (Helena Biosciences Europe; Gateshead, UK) which was used as the reference instrument.

The investigators duplicated the platelet aggregation study using the CS-2000i analyzer (Sysmex Corporation; Kobe, Japan) with the same agonists. The CS-2000i is an open analytical system, which means that test protocols and reagents can be user-defined. For this study, test protocols varying only in the reagent/concentration used were defined for commonly used platelet agonists. These protocols facilitated the generation of measured raw light transmission data under highly controlled conditions including sample volume, incubation period, reagent volume, reaction mixture stirrer speed and period of time for which the reaction was to be monitored.

CS-2000i reaction cuvette stirrer speed was found to influence reaction sensitivity and was optimized to 800 revolutions per minute (rpm). There were no clinically significant changes in aggregation response when the PRP platelet count was 150 to 480 × 109/L, but below this there were changes in the maximum amplitude (MA) and slope (rate). Dose response with each of the agonists was comparable between CS-2000i and an AggRAM aggregometer and normal subjects receiving antiplatelet drugs. Aggregation imprecision was similar on both the CS-2000i and AggRAM systems, with a coefficient of variation for 2 μm to 5 μm ADP MA and slope varying between 3% to 12%.

The authors concluded that their data demonstrated that CS-2000i is comparable to a stand-alone aggregometer, although CS-2000i has the advantages of walk-away technology and also required a 44% smaller sample volume than the AggRAM. The study was published July 13, 2014, in the International Journal of Laboratory Hematology.

Related Links:

University College London 
Helena Biosciences Europe 
Sysmex Corporation


Gold Member
Chagas Disease Test
CHAGAS Cassette
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Fecal DNA Extraction Kit
QIAamp PowerFecal Pro DNA Kit
New
Troponin I Test
Quidel Triage Troponin I Test
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Immunology

view channel
Image: The findings were based on patients from the ADAURA clinical trial of the targeted therapy osimertinib for patients with NSCLC with EGFR-activated mutations (Photo courtesy of YSM Multimedia Team)

Post-Treatment Blood Test Could Inform Future Cancer Therapy Decisions

In the ongoing advancement of personalized medicine, a new study has provided evidence supporting the use of a tool that detects cancer-derived molecules in the blood of lung cancer patients years after... Read more

Microbiology

view channel
Image: Schematic representation illustrating the key findings of the study (Photo courtesy of UNIST)

Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours

Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Sekisui Diagnostics UK Ltd.