New Kit Extracts Genomic DNA from Blood

An innovative kit can extract genomic DNA from fresh or frozen blood samples in just a few minutes.

The kit uses a nucleic acid binding matrix that easily inserts into either a pipette, which is best for extraction of a few samples or with system for extraction workflows that require a higher level of throughput.

The TruTip technology can be adapted to work with most other standard electronic pipettes and automated liquid handling systems. The samples are passed back and forth across the binding matrix multiple times. TruTip delivers inhibitor-free, polymerase chain reaction (PCR)-ready, human genomic DNA from both saliva and whole blood, faster and more efficiently than other extraction kits on the market today.

The TruTip approach for extraction of human genomic DNA from blood is five times faster than spin columns when automated and does not require centrifugation, vacuum manifolds, or magnets. Samples from 50 µL to 400 µL can easily be extracted in just minutes allowing molecular biologists and clinical laboratories to achieve results equivalent to "gold-standard” methods, in a fraction of the time. The patented TruTip is manufactured by Akonni Biosystems, (Frederick, MD, USA), and is easily inserted into either a Rainin EDP 3-Plus pipette (Rainin Instrument LLC; Oakland, CA, USA) or into an Eppendorf epMotion system (Eppendorf; Hamburg, Germany).

TruTip kits are also in development for extraction of a variety of other nucleic acid targets from a range of clinical samples, such as sputum, nasal wash, urine, stool, or CSF, where faster purification methods are being required by accelerating infectious disease testing to prevent bottlenecks.

Related Links:
Akonni Biosystems
Rainin Instrument LLC.
Eppendorf