Cell Sorter Facilitates Multicolor Flow Cytometry Studies

A next generation cell sorter uses stable fluidic design and electronic processing while expanding sorting capabilities with an array of laser options.

Complex, multicolor sorting is enabled by seven pinholes that spatially separate each of the seven lasers. A range of unique software components further enhances instrument functionality.

IntelliSort II is a bead-less drop delay determination and monitoring system. The multifiber Beam Shaping Optic creates flat-top beam profiles, providing short alignment times and high-level optic stability. Two stages control the alignment for all seven lasers to simplify sorter preparation. Camera images and proprietary algorithms determine optimal drop delay without fluorescent beads being incorporated in the sample line. A quick-release nozzle design enables easy removal or swapping of the nozzle body and tip through the use of a single lever

The IntelliSort II is produced by Beckman Coulter Inc., (Brea, CA, USA) and uses the MoFlo Astrios technology. The auto-quality-control allows system performance to be tracked and trended across 32 available parameters, while the updated Summit Software permits six-way sorting into tubes and sorting into plates having as many as 1,536 wells. Index-sorting logic tracks each plate-sorted cell back to its histogram or scatter plot. A touchscreen control panel facilitates daily set-up with operations designed around core laboratory workflow. Biosafety features are prominent on the system.

Brad Calvin, vice president, Flow Cytometry at Beckman Coulter, said, "The range of features incorporated on MoFlo Astrios elevates the sorting and productivity capabilities available. This instrument reflects our commitment to providing scientists with instruments that combine cutting-edge performance with workflow gains.”

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Beckman Coulter