Biofluid Ribonucleic Acid Isolation Kit Launched

An isolation kit for the extraction of small ribonucleic acid (RNA) from serum, plasma, urine and other biofluids has been specifically optimized for such applications.

The new biofluids kit allows scientists to obtain high quality microRNA from virtually all types of biofluids in just 45 minutes and the workflow is completed in six simple steps without the use of hazardous chemicals such as phenol and chloroform.

The miRCURY RNA Isolation Kit – Biofluids (Exiqon; Vedbaek, Denmark) is optimized to work with company's highly sensitive miRCURY LNA Universal real-time microRNA polymerase chain reaction (qPCR) platform. The Isolation Kit is ideal for use with urine samples together with Exiqon’s Toxicology Focus microRNA PCR Panels, and for serum and plasma samples with the Serum/Plasma Focus microRNA PCR Panels, or indeed any of the almost 2,000 prevalidated qPCR microRNA assays available. The Isolation Kit – Biofluids can be used with a centrifuge or a vacuum manifold is provided in pack sizes of 10 and 50 units.

The company offers a complete range of PCR products for microRNA analysis, which includes ready-to-use panels for genome-wide screening of human, mouse and rat microRNAs, numerous focus panels, the fully flexible Pick & Mix panels, spike-in kits, cDNA synthesis and PCR Master Mix kits, as well as unique qPCR data analysis software. Customers that have identified novel microRNAs can take advantage of Exiqon’s proprietary LNA chemistry and unique design algorithm through easy-to-use online tool for high quality design of custom microRNA qPCR assays.

Henrik M. Pfundheller, Senior Vice President Sales and Marketing at Exiqon, said, "Our customers want fully integrated systems that allow highly robust and sensitive microRNA profiling in difficult samples such as blood and urine, and we deliver the solution with this new kit. We have not only optimized the protocols to ensure the highest yield in the market but have also made sure that the sample does not contain any inhibitors which may jeopardize the downstream PCR analysis."

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