Stool DNA Extraction Kit for Molecular Diagnostics Faster, Simpler

A DNA extraction kit provides a faster, simpler method to purify genomic DNA from stool samples prior to PCR amplification and analysis.

The E-Sphere stool DNA extraction kit answers the market need for speed with the added benefits of accuracy, improved cost effectiveness, reduced risk of sample contamination, and greater safety for lab technicians. The process requires only standard clinical laboratory equipment.


Advanced technology in the kit includes the high performance E-Sphere stool enzyme, a thermostable reagent that digests fecal matter at 75 °C during the 15-minute incubation stage. A further innovation is the proprietary E-Sphere clean-up column, which purifies the DNA.

Phthisis Diagnostics (Charlottesville, VA, USA) introduced the kit at the American Society for Microbiology annual meeting, which took place during May 21-24, 2011, in New Orleans (LA, USA). The kit allows lab workers to extract DNA from human stool samples in a three-step process. Other similar kits take three times the steps, said company cofounder and president Crystal Icenhour.

The E-Sphere stool sample-preparation method allows manual DNA extraction from 12 stool samples in less than 30 minutes. The process includes only nine steps and minimizes sample contamination risk with just 2 sample transfers. Stool samples can be quickly processed within a biological safety cabinet.

The kit was developed and manufactured in the USA to International Organization for Standardization (ISO) 9001 & ISO 13485 standards in a Quality Systems Registrars (QSR) & current Good Manufacturing Practice (cGMP) compliant facility, and is designated as a general-purpose reagent.

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Phthisis Diagnostics