Multi-Pathogen Tests Optimized for Two Automated Systems

Tests based on multiplexing polymerase chain reaction (PCR) technology, which enable detection of multiple pathogens in a single tube, have been optimized for two automated systems. This will allow these tests to be performed throughout a wide spectrum of labs, from small to mid-sized labs to large commercial reference labs.

Called Seeplex and developed by Seegene (Rockville, MD, USA, and Seoul, Republic of Korea), the tests deliver maximum specificity, reproducibility, and sensitivity and can be applied to a broad range of molecular diagnostics, including human, animal, plant, and microorganism. Currently, Seegene's Seeplex multi-pathogen detection tests offer labs worldwide simple, cost-effective, and comprehensive screening for sexually transmitted diseases (STDs), respiratory viruses, human Papillomaviruses, sepsis, and pneumonia.

The Seeplex multi-pathogen tests are now optimized for Lab 901's (Midlothian, UK) ScreenTape and Caliper LifeSciences' (Hopkinton, MA, USA) LC90 automated detection systems. The ScreenTape system is the first fully automated, walk-away solution for gel electrophoresis. ScreenTape will automate the simultaneous analysis of eight or sixteen Seeplex PCR samples. The processing speed for eight samples is less than 10 minutes; 16 samples less than 15 minutes. ScreenTape displays results using easy to interpret color codes. The ScreenTape system comprises the TapeStation (that carries out liquid handling, electrophoresis, and imaging), ScreenTape (a consumable that contains the pre-cast, pre-packaged gel and running buffer), and custom software. With no gel or buffer preparation and no system priming, even untrained operators can rapidly generate accurate and reproducible test data.

The LabChip 90 (LC90) system performs fast, automated, 1-D electrophoretic separations of protein, DNA, and RNA samples directly from a 96 or 384 well plate. The LC90 can load and read 96 Seeplex samples within 45 minutes or 384 Seeplex samples in four hours in easy-to-interpret reports.

Seegene is currently working to optimize Seeplex tests for other automated capillary electrophoresis systems. Seeplex's compatibility with a wide range of automated detection systems will provide end-users with the flexibility to use the platform best suited for their purposes.

Seegene applies its novel and proprietary Seeplex system utilizing DPO (dual priming oligo) and ACP (annealing control primer) to create multi-pathogen tests delivering maximum specificity, reproducibility, and sensitivity. Seegene's mission is to integrate Seeplex with disease diagnostics to provide a new guideline for effectively treating patients


Related Links:
Seegene
Lab 901
Caliper LifeSciences