We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.


Download Mobile App
Recent News COVID-19 Clinical Chem. Molecular Diagnostics Hematology Immunology Microbiology Pathology Technology BioResearch Industry

Researchers Developing 'Lab-in-Box' Kit for Medium Throughput Processing of COVID-19 Samples for Detection Using RT-PCR

By LabMedica International staff writers
Posted on 10 Nov 2020
A team of scientists is developing viral RNA extraction kits for medium-throughput processing of COVID-19 samples for detection using RT-PCR.

Magnostics Ltd. (Dublin, Ireland) is leading a consortium of scientists to develop and launch viral RNA extraction kits for the detection of COVID-19. To detect the SARS-CoV-2 virus, which causes COVID-19 infection, a very small number of RNA viral genomes must be isolated from the nasal swab. This is a stereotypical needle in the haystack problem where there are a very small number of viruses among tens of thousands of much larger cells. The team of scientists has developed necessary protocols for SARS-cov-2 testing based on Magnostics' Si@Hi-Mag superparamagnetic beads. The key is to use Si@Hi-mag magnetic nanoparticles to capture the RNA from the complicated soup that makes up the virus lysate, which includes proteins, lipids, and other cellular components, and then use a powerful magnet to move and thus separate the magnetic nanoparticles. This allows a series of solutions to be used to rinse the RNA-nanoparticle complex and then release the RNA into a buffer that can be used for reverse transcription and quantitative detection with the polymerase chain reaction (PCR).


Using test samples from patients, the team has proven that Si@Hi-mag beads can be used to extract viral RNA from clinical samples. This includes Lysis buffer, which inactivates and bursts open the virus, superparamagnetic nanoparticles, and a rare-earth magnet array for medium-throughput processing. This important demonstration forms the basis for Magnostics to develop a CE marked 'lab-in-box' kit for the medium throughput manual extraction of viral RNA from clinical samples. The product will allow clinical laboratories to perform 400-600 extractions per day using existing infrastructure.

Related Links:
Magnostics Ltd.

Latest COVID-19 News