Novel Filtration System Removes Proteins and Phospholipids from Analytical Samples

Biotechnology and other life science researchers now have available a novel filter system that removes both proteins and phospholipids, which leaves samples ready for LC-MS/MS (liquid chromatography-mass spectroscopy) analysis.

The Biotage (Uppsala, Sweden) ISOLUTE PLD+ plate combines protein and phospholipid removal from blood-based matrix samples and provides simple and effective cleanup for LC-MS/MS analysis. According to the manufacturer, ISOLUTE PLD+ plates remove more than 99% of plasma proteins and phospholipids, the main causes of ion suppression, leading to cleaner extracts and increased sensitivity, signal-to-noise, for a broad range of analytes.


ISOLUTE PLD+ plates are available in standard 96-well format and can handle sample volumes of 100 to 200 microliters. Operation of the ISOLUTE PLD+ plate includes a filtration step that can be accomplished with a 96-well compatible positive pressure manifold (such as the Biotage Pressure+ 96), a vacuum manifold (for example the Biotage VacMaster 96) and most automated liquid handling systems.

“Requiring next to no method development, ISOLUTE PLD+ can be integrated quickly and easily into routine workflow, increasing productivity and reducing instrument downtime,” said Paul Roberts, analytical product manager at Biotage.

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