New Single Step ELISA Kits Available for Multiple Analytes

A new line of ELISA kits with a universal simplified protocol is now available for biotech and life science researchers.

The Abcam plc. (Cambridge, United Kingdom) line of SimpleStep ELISA kits currently comprise kits for the detection of more than 35 molecules ranging from interleukins and interferons to tumor markers.

Abcam states that the new single wash, colorimetric sandwich ELISA kits have a greatly simplified protocol when compared to standard multistep ELISAs, providing significant timesaving and ease of use. This is accomplished by employing a labeled capture and detection antibody which, "immunocapture" the specific analyte from the sample solution. This entire complex (capture antibody/protein/detection antibody) is in turn immobilized in the well of the microtiter plate via the immunoaffinity of an anti-tag antibody coated in the microplate well.

The unified operational protocol calls for a single one-hour incubation at room temperature and a single wash step. Furthermore, the new SimpleStep platform requires no specific training or new instrumentation.

James Murray, general manager of Abcam, said, “Abcam is continually in touch with researchers to anticipate the latest research trends and developments, so that we can continue to meet their needs worldwide. We are excited to see how SimpleStep ELISA kits will help to increase the pace of scientific discoveries.”

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