New Line of DNA Amplification Reagents Promises Consistent Results Across All PCR Instruments

New reagent mixes specially formulated for PCR applications are now available for biotech and genomics researchers.

Bio-Rad Laboratories, Inc. (Hercules, CA, USA) recently launched two iTaq universal one-step kits aimed to deliver superior data precision and highly efficient reverse transcription quantitative PCR (RT-qPCR) performance under most reaction conditions on any qPCR instrument.


The new iTaq universal one-step kit line includes the iTaq universal SYBR Green one-step kit and the iTaq universal probes one-step kit. Both kits come as a double concentrated, ready-to-use reaction mixes with iScript reverse transcriptase optimized for RT-qPCR.

The iTaq universal SYBR Green one-step kit includes SYBR Green I dye and Bio-Rad’s patented RT inhibitor reducer. The iTaq universal probes one-step kit allows researchers to combine multiple targets in one well (multiplexing), improving data precision and reducing costs.

The new kits enable investigators to obtain reliable and reproducible gene expression-analysis results while amplifying genomic DNA and difficult amplicons with superior efficiency. Results are consistent across real-time instrument platforms under fast or standard conditions.

The Bio-Rad Kits are intended for gene expression analysis, genotyping, mutation detection, and pathogen detection.

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