Analytic Device May Detect Bacterial Virus Particles

Newly developed technology provides for the precise measurement of nanoparticles.

The University of California, Santa Barbara (UCSB; USA) researchers have devised a new instrument capable of detecting individual nanoparticles with diameters as small as a few tens of nanometers. The study was published on line in March 2011 in the journal Nature Nanotechnology, and is slated to appear in the April 2011 print issue.

"This device opens up a wide range of potential applications in nanoparticle analysis,” said Dr. Jean-Luc Fraikin, the lead author on the study. "Applications in water analysis, pharmaceutical development, and other biomedical areas are likely to be developed using this new technology.”

The instrument was developed in the lab of Dr. Andrew Cleland, professor of physics at UCSB, in collaboration with the group of Dr. Erkki Ruoslahti, professor, Sanford-Burnham Medical Research Institute at UCSB. Dr. Fraikin is presently a postdoctoral associate in the Marth Lab at the Sanford-Burnham Medical Research Institute's Center for Nanomedicine, and in the Soh Lab in the department of mechanical engineering at UCSB.

The device detects the tiny particles, suspended in fluid, as they flow one by one through the instrument at rates estimated to be as high as half a million particles per second. Dr. Fraikin compares the device to a nanoscale turnstile, which can count--and measure--particles as they pass individually through the electronic "eye” of the device. The instrument measures the volume of each nanoparticle, allowing for very rapid and precise size analysis of complex mixtures. Moreover, the researchers demonstrated that the instrument could detect bacterial virus particles, both in saline solution as well as in mouse blood plasma.

In this study, the researchers additionally found an amazingly high concentration of nanoparticles present in the native blood plasma. These particles exhibited an intriguing size distribution, with particle concentration increasing as the diameter fell to an order of 30 nm to 40 nm, an as-yet unexplained result.


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