Easy and Cost-Effective Production of Functional siRNA Achieved with Generation Kit

A siRNA generation kit enables easy and cost-effective generation of a large number of small interfering RNAs (siRNAs) from full-length target genes.

siRNAs are 21-23-nucleotide RNA molecules that can cause targeted gene silencing in mammalian cells through a process known as RNA interference. In nature, siRNAs are generated by ribonuclease III cleavage of longer double-stranded RNAs (dsRNAs). When dsRNAs are transfected directly into mammalian cells, they activate the interferon system and provoke nonspecific gene suppression and cytotoxic response. siRNAs have proven to be effective at specifically silencing gene expression without causing any interferon response.

Using an ultra-active form of human recombinant dicer enzyme, a double-stranded RNA-specific endonuclease, it is possible using the kit to cleave more than 95% of dsRNA template into 22 bp [base pair] siRNAs within two hours under optimized reaction conditions. In contrast to individual synthetic siRNAs, the Turbo Dicer kit allows the user to quickly produce multiple siRNA species against the target mitochondrial RNA (mRNA), and thereby achieve effective gene silencing with minimal guesswork. Moreover, due to the minimal concentration of any individual siRNA species generated with the Turbo Dicer enzyme, off-target effects are not a problem.

For maximum convenience, the Turbo Dicer siRNA generation kit, developed by from Amsbio (Abingdon, UK), contains everything that is required for preparing double-stranded RNA from the user's target gene(s), dsRNA cleavage, siRNA cleanup, and transfection. Both the Recombinant Turbo Dicer enzyme and the RNA purification columns are also available separately.

AMS Biotechnology (Amsbio) is a leading international provider of products and custom services for life sciences research. The company's range includes over 23,000 polyclonal and monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, tissue DNA, RNA, protein, and microarray products. Key research areas include apoptosis, cell invasion and migration, cell signaling, DNA damage, electrophoresis, glycobiology, posttranslational modification, and stem cell biology.

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