DNA Polymerase Can Be Stored at 4 °C

A new DNA polymerase can be stored in the refrigerator at 4 °C. The storage buffer of the DNA polymerase contains a stabilizer that binds to the enzyme preventing inactivation. This enables reaction setup without the use of ice--saving the time usually required for reagent thawing.

Called the TopTaq DNA polymerase, it has a number of convenient features that provide high-product yields in a wide range of end-point polymerase chain reaction (PCR) applications. The benefits of TopTaq include room temperature handling, and a single preoptimized protocol suitable for all PCR assays.

The buffer formulation and single preoptimized protocol eliminate the need for optimization of experimental parameters for individual-primer template systems, saving time and money. High yields of specific amplification product are achieved, even when amplifying a range of different sized products using the same Mg2+ concentrations and annealing temperatures.

A product of Qiagen (Hamburg, Germany), TopTaq DNA polymerase is supplied with CoralLoad concentrate, which can be optionally added for immediate loading of amplified products onto a gel. Q-Solution is also provided for improved amplification of GC-rich templates.


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