Immuno-Capture Technology Optimized for Mass Spectrometer Detection

A next-generation immuno-capture technology has been optimized for mass spectrometer (MS) detection. Immuno-catch is a powerful alternative to slower, low-yield immunoprecipitation assays. The novel technology offers a promise of molecular diagnostics for the detection using standard MS and gel technologies and new capabilities for advanced peptide and protein research.

The new kit consists of streptavidin-coated 96 well microplates and all the necessary buffers for binding and elution from the plate. The streptavidin coating procedure provides a high binding capacity for biotinylated capture agents, including antibodies, but also an extremely robust coating to allow easy elution of captured analytes. These qualities provide a high-throughput, multiplex format for the enrichment of specific target proteins and peptides from complex biological samples (such as blood serum).

PerkinElmer Life and Analytical Sciences (Waltham, MA, USA) developed the new kit, which is called the Immuno-catch kit. In addition to conventional immunoassays of intact proteins, the kit was designed to meet the unique requirements of investigators interested in the low-molecular weight peptidomic environment. Researchers in the emerging discipline of peptidomics are very interested in peptide fragments as important markers of cellular signals and as potential markers of diagnostic information.

PerkinElmer is currently collaborating with Drs. Lance Liotta and Emanuel Petricoin, co-directors of the Center for Applied Proteomics and Molecular Medicine at George Mason University (Fairfax County, VA, USA) for the investigation of possible clinical utility of peptidomics and Immuno-catch. According to Dr Liotta, "The Immuno-catch platform provides a critical component for our ongoing alliance with PerkinElmer to explore the peptidome, which we hypothesize to hold untapped and significant diagnostic potential.

In addition to conventional immunoassays of intact proteins, the Immuno-catch kit was designed to meet the unique requirements of investigators interested in the low-molecular weight peptidomic environment. Researchers in the emerging discipline of peptidomics have great interest in peptide fragments as important markers of cellular signals and as potential markers of diagnostic information.


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