Kit Analyzes Human DNA for Forensic Laboratories

A commercial reagent kit is being developed for analyzing degraded or limited DNA. In addition, a system is being developed with new software for automating DNA testing processes to streamline forensic laboratory workflow, as well as a new software application for expediting the process of laboratory validation.

DNA contained within biologic evidence found at a crime scene is a powerful tool for identifying and excluding potential contributors. Similar to fingerprinting, DNA testing can identify and differentiate between individuals. Because DNA can be the ultimate proof of identity, DNA analysis systems have become a significant part of the investigative process.

Forensic laboratories use an integrated suite of DNA analysis technologies, instrumentation, and human identification analysis software to effectively process DNA collected at crime scenes. Each of these products and technologies were designed to work in unison to strengthen a crime laboratory's capacity to process reproducible and defensible DNA samples and eliminate sample backlogs. These technologies were developed to enable forensic technicians to more efficiently use DNA in solving crimes, exonerating the wrongly accused and identifying missing persons.

Applied Biosystems (Foster City, CA, USA) reported that it expects the AmpF STR MiniFiler polymerase chain reaction (PCR) amplification kit for degraded DNA, the Hid Evolution system, and Valid Software will help the more than 10,000 forensic laboratories around the world maximize the use of DNA in human identification.

"Because DNA can be found virtually everywhere, it has become a great resource in providing information that aids the investigative process, and there are great demands on forensic labs to process samples in a quality, timely manner that meets regulatory requirements,” said Kevin Lothridge, executive director of the National Forensic Science Technology Center (NFSTC; Largo, Fl, USA). "With tools that expedite and expand the forensic laboratory's capacity to make positive identification, we can better support law enforcement and public safety professionals in their efforts to prosecute the guilty and exonerate the innocent.”

The MiniFiler kit is the first commercially available DNA testing kit that enables forensic DNA analysts to generate more useful information from degraded DNA as well as from samples that are limited by an impurity also known as inhibited DNA samples. The genetic information obtained can then be used in criminal, missing persons, and mass disaster cases. The MiniFiler kit is expected to be available in March 2007.

Applied Biosystems has formed a collaboration with Tecan (Männedorf, Switzerland), a manufacturer of advanced automation solutions, to co-develop and co-market the new Hid Evolution system, an automated DNA workstation with new software designed to streamline routine casework sample workflow for human identification applications. The Hid Evolution system integrates the Tecan Freedom Evo liquid handling workstation with the Applied Biosystems 7500 Real-Time PCR System, 3130xl genetic analyzer and AmpF Str DNA testing kits. By incorporating automated liquid handling and data transfer into forensic DNA testing laboratories, the Hid Evolution system reduces the labor required to process casework samples while decreasing the turnaround time. The system is expected to be available in March 2007.

Applied Biosystems is also delivering a new software application for forensic DNA laboratories that is expected to reduce the amount of labor associated with conducting validation studies required to implement new forensic DNA technology, such as the MiniFilerkit. Validsoftware is designed to help support, simplify, and standardize validation studies, while meeting government guidelines, which oversee the standards for
forensic DNA lab operation. Validsoftware is expected to be available in early summer 2007.


Related Links:
Applied Biosystems
NFSTC
Tecan