New Series of Lab-on-a-Chip Assays

Eighteen new or upgraded assay kits for RNA, DNA, and protein have been developed that offer improved sensitivity, sizing range, robustness, and sample matrix tolerance.

The Series II family of assay kits is designed for use with the Agilent 2100 bioanalyzer platform of Agilent Technologies (Palo Alto, CA, USA). In addition to improved specifications, the new kits have an extended shelf-life of a minimum of four months, which is expected to grow with further testing. There will be no need to purchase separate ladders for the RNA assay. The existing kits will be phased out by June 2006.

The Series II protein assays facilitate the replacement of time-consuming sodium dodecyl (lauryl) sulphate-polyacrylamide gel (SDS-PAGE) and capillary gel electrophoresis with much faster fluidic analyses. The Protein 230 kit features an increased sizing range, higher sensitivity and salt tolerance. The Protein 80 kit can be used for analyzing proteins from 5-80 kDa, making it suitable for reduced antibodies. Primary improvements to the three DNA Series II assays include better sizing and quantification, better sensitivity specifications, increased stability and robustness, and streamlined protocol. The RNA and protein kits are boxed in two parts for storage at 4 or -20ºC.



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