Labeling Kits for DNA Microarray Uses

A new line of labeling kits for DNA microarray applications provides users with all the reagents necessary to go from total RNA samples to microarrays.

These RNA ampULSe kits, from Kreatech Biotechnology (Amsterdam, The Netherlands), combine technology obtained from a technology supply agreement between the company and Ambion (Austin, TX, USA) for Ambion's Message Amp II aRNA amplification system in conjunction with Kreatech's proprietary universal linkage (ULS) system and labeling kits for gene-expression analysis.

The kits offer the ULS labeling molecule bound to a choice of fluorescent dye pairs that have been licensed by Kreatech from other companies. The RNA ampULSe kit also includes Kreatech's KREA pure columns to purify the labeled aRNA, which insures effective removal of unreacted ULS and higher recoveries of sample. Kreatech's ULS technology relies on the special binding properties of transition metals, especially platinum, to form coordinative bonds with biomolecules by binding to specific sites on DNA, RNA, and proteins.

"In using the direct, non-enzymatic ULS labeling technology, the performance of the Ambion in vitro transcription reaction is further enhanced, since the end user can use natural unmodified nucleotides in the amplification step, resulting in higher sample yields and a better representation of the transcriptome,” explained Brent Keller, general manager, Kreatech USA/vice president, commercial operations.




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