Master Mixes for PCR Instruments
By Biotechdaily staff writers
Posted on 01 Aug 2005
Ready-to-use reagent mixes simplify the preparation of samples for quantitative polymerase chain reaction (qPCR) and two-step quantitative reverse transcription PCR (qRT-PCR).Posted on 01 Aug 2005
The FastStart TaqMan Probe Master and FastStart SYBR Green Master mixes are optimized for use with most real-time PCR instruments other than the LightCycler 2.0 instruments. Available in two formulations, one contains the ROX reference dye and one does not. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart TaqMan Probe Master allows very sensitive detection and quantification of defined DNA sequences. The FastStart TaqMan Probe Master works well in combination with the probes of the Universal ProbeLibrary of Roche Diagnostics (Basel, Switzerland), which developed the new mixes.
The FastStart SYBR Green Master is a ready-to-use double-concentrated master mix that contains all the necessary reagents (except primers and template) and is suitable for quantitative real-time DNA detection assays whenever qPCR and two-step qRT-PCR assays in the SYBR Green I detection format are performed. In principle, the TaqMan Probe Master and the SYBR Green Master can be used for the amplification and detection of any DNA or cDNA target.
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Roche-Diagnostics