Reagents for GeneChip Expression Array
By Biotechdaily staff writers
Posted on 12 Feb 2004
A new line of GeneChip expression reagents has been developed, offering a complete, standardized sample preparation system that is easier to use and will help customers produce more robust and consistent array results, whether used for diagnostic biopsies or bioresearch.Posted on 12 Feb 2004
The reagents were developed for use with the GeneChip IVT Labeling Kit of Affymetrix (Santa Clara, CA, USA) in collaboration with Invitrogen Corp. (Carlsbad, CA, USA). The reagents include two new complementary DNA (cDNA) synthesis kits that contain Invitrogen's SuperScript reverse transcriptase (RT). The One-Cycle cDNA synthesis kit offers all necessary reagents for standard target labeling, using as little as 1 ug of total RNA as starting material prior to the labeling step. The Two-Cycle cDNA kit offers customers a streamlined procedure for preparing samples using a small amount of material, such as biopsy or laser capture dissected samples. The entire procedure takes only two and a half days, compared to four days with the previous procedure.
The new GeneChip IVT Labeling Kit was developed specifically for the GeneChip platform to provide high-yield, consistent, and uniform labeling efficiency, with more streamlined protocol and convenient packaging.
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