Seegene Highlights High-Throughput, Single Tube 8-Plex RT-PCR Test for Flu A, Flu B, RSV and COVID-19 with Dual Internal Controls

Seegene, Inc. (Seoul, Korea) highlighted its new high-throughput 8-plex test for Flu A, Flu B, RSV and COVID-19 with dual internal controls at the all virtual 2020 AACC Annual Scientific Meeting held on December 13-17.

Attendees of the 2020 AACC Virtual Meeting Platform viewed Seegene’s Allplex SARS-CoV-2/FluA/FluB/RSV Assay, a single tube real-time RT-PCR assay that simultaneously detects and differentiates Flu A, Flu B, RSV A/B, and three different target genes of COVID-19 (S gene, RdRP gene and N gene).


Given the similarity in terms of symptoms between seasonal flu and SARS-CoV-2, multiplexing capability will be a critical consideration to accommodate high volume testing necessary for effective disease control in preparation for flu season during COVID-19 pandemic. Seegene's assay incorporates its proprietary high multiplex chemistry technologies, maximizing sensitivity and specificity during PCR amplification and detection of multiple targets. Combined with its unique task target automated system and interpretation software solution, the company offers unparalleled capacity at the most competitive pricing available on the market.

The assay also includes dual targets for internal control (exogenous and endogenous, respectively) run in the same reaction tube, which allow verification of the whole test process as well as proper sampling. The assay is currently validated with a wide range of extraction systems (Seegene STARlet, Seegene NIMBUS, Microlab STARlet IVD, Microlab NIMBUS, Seeprep32, KingFisher Flex, MagNA Pure 96, NucliSENS EasyMag, GeneAll Ribospin vRD Viral RNA/DNA Extraction Kit, QIAamp DSP Viral RNA Mini kit) and plans to expand further upon market demand. For PCR instruments, Bio-Rad CFX96 systems can be used.

Seegene also offered a glimpse of its comprehensive syndromic product portfolio on the same automated solution, including its well-established syndromic respiratory virus/bacteria panels which simultaneously detect 26 different targets.