Paper-Based Electrochemical Device Analyses Glucose in Urine
|
By LabMedica International staff writers Posted on 07 May 2012 |
|
A paper-based electrochemical system has been designed for flow-injection analysis of the concentration of glucose in urine samples.
Capillary wicking facilitates a gravity-driven flow of buffer solution continuously through paper and nitrocellulose, from a buffer reservoir at one end of the device to a sink at the other.
Scientists at the Vrije Universiteit (VU University; Amsterdam, The Netherlands) devised the instrument whereby difference in height between the reservoir and the sink leads to a continuous and constant flow. The nitrocellulose, on which glucose oxidase is immobilized, lies horizontally on a working electrode, which consists of a thin platinum layer deposited on a solid support. The counter and reference electrodes are strategically positioned upstream in the buffer reservoir.
A simple pipetting device was developed for reliable application of sub-microliter volumes of sample without the need of commercial micropipettes, which damage the nitrocellulose membrane. The system is based on amperometry, and the measured current is proportional to the glucose concentration. The system was tested for the determination of the concentration of glucose in 15 urine samples that had tested positive for glucose during routine analysis in the clinical laboratory. The concentration measured by the devise method resulted in a noninvasive, quantitative assay that could be used for diagnosis and monitoring of diabetes.
The system can also be used to measure glucose in other fluids, such as cell culture medium. Use of enzymes other than glucose oxidase, e.g., cholesterol oxidase, lactate oxidase, or d-3-hydroxybutyrate dehydrogenase, will enable the measurement of other metabolites, such as cholesterol, lactate, or d-3-hydroxybutyrate, respectively, making this system versatile for general clinical applications.
The authors concluded that this method does not require disposable test strips, with enzyme and electrodes that are thrown away after each measurement. Due to its low cost, this system could be used in medical environments that are resource-limited. The study was published on April 5, 2012, in the journal Analytical Chemistry.
Related Links:
Vrije Universiteit
Capillary wicking facilitates a gravity-driven flow of buffer solution continuously through paper and nitrocellulose, from a buffer reservoir at one end of the device to a sink at the other.
Scientists at the Vrije Universiteit (VU University; Amsterdam, The Netherlands) devised the instrument whereby difference in height between the reservoir and the sink leads to a continuous and constant flow. The nitrocellulose, on which glucose oxidase is immobilized, lies horizontally on a working electrode, which consists of a thin platinum layer deposited on a solid support. The counter and reference electrodes are strategically positioned upstream in the buffer reservoir.
A simple pipetting device was developed for reliable application of sub-microliter volumes of sample without the need of commercial micropipettes, which damage the nitrocellulose membrane. The system is based on amperometry, and the measured current is proportional to the glucose concentration. The system was tested for the determination of the concentration of glucose in 15 urine samples that had tested positive for glucose during routine analysis in the clinical laboratory. The concentration measured by the devise method resulted in a noninvasive, quantitative assay that could be used for diagnosis and monitoring of diabetes.
The system can also be used to measure glucose in other fluids, such as cell culture medium. Use of enzymes other than glucose oxidase, e.g., cholesterol oxidase, lactate oxidase, or d-3-hydroxybutyrate dehydrogenase, will enable the measurement of other metabolites, such as cholesterol, lactate, or d-3-hydroxybutyrate, respectively, making this system versatile for general clinical applications.
The authors concluded that this method does not require disposable test strips, with enzyme and electrodes that are thrown away after each measurement. Due to its low cost, this system could be used in medical environments that are resource-limited. The study was published on April 5, 2012, in the journal Analytical Chemistry.
Related Links:
Vrije Universiteit
Gold Member
STI Test
Vivalytic MG, MH, UP/UU
Immunofluorescence Analyzer
IFA System
Hematology Consumables
Bioblood Devices
Latest Clinical Chem. News
- Saliva-Based Test Detects Biochemical Signs of Sleep Loss
- Simple Dual-Tau Blood Test Detects and Stages Alzheimer’s Disease
- Alzheimer’s Blood Biomarkers Linked to Early Cognitive Differences Before Dementia
- Urine-Based Test Shows Promise for Autism Screening in Children
- Blood-Based Sensor Detects Early Signs of Alzheimer’s and Parkinson’s
- Liquid Biopsy Biomarkers May Improve Childhood Epilepsy Diagnosis
- Urine-Based Alzheimer’s Test Receives FDA Breakthrough Device Designation
- Fluid Biomarker Improves Diagnosis and Monitoring of Primary CNS Lymphoma
- New CA19-9 Cutoff Value Helps Identify High-Risk Pancreatic Cancer Patients
- Blood-Based Biomarkers Show Promise for Psychosis Risk Prediction
- International Experts Recommend Ending Routine 'Corrected' Calcium Reporting
- Long-Term Data Show PSA Screening Modestly Reduces Prostate Cancer Deaths
- Urine-Based Nanosensor Tracks Lung Cancer and Fibrosis Noninvasively
- FDA-Cleared Assay Enables Comprehensive Automated Testosterone Testing
- CE-Marked Blood Biomarker Test Advances Automated Alzheimer’s Diagnostics
- Blood-Based Alzheimer’s Test Gains CE Mark for Amyloid Pathology Detection
Channels
Molecular Diagnostics
view channel
New PCR Assay Supports Bundibugyo Ebola Outbreak Surveillance
Rapid identification of Ebola infections is essential to limit transmission and guide public health response, yet detection can be difficult when outbreaks involve rare variants. The current outbreaks... Read more
Plasma Protein Signature Predicts Lung Cancer Risk Up to Five Years Ahead
Lung cancer remains a leading cause of cancer death, and many cases are detected only after symptoms appear. Current screening programs largely target people with a history of smoking, leaving other at-risk... Read more
Hematology
view channel
Next-Generation Hematology Platform Streamlines High-Complexity Lab Workflows
Sysmex America (Chicago, IL, USA) has introduced the next generation XR-Series, centered on the XR-10 Automated Hematology Module for high-complexity laboratories. The platform builds on the widely used... Read more
Blood Eosinophil Count May Predict Cancer Immunotherapy Response and Toxicity
Immune checkpoint inhibitors have improved outcomes across many cancers, yet only a subset of patients derive durable benefit and biomarkers to guide treatment remain limited. Eosinophils, best known for... Read more
Immunology
view channel
Aptamer-Based Biosensor Enables Mutation-Resilient SARS-CoV-2 Detection
Rapid evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can undermine existing molecular diagnostics, especially when assays target small viral components. Double-antibody sandwich... Read more
Study Points to Autoimmune Pathway Behind Long COVID Symptoms
Long COVID leaves many SARS-CoV-2 survivors with persistent fatigue, cognitive issues, palpitations, and musculoskeletal pain for months or years. Estimates cited in new research suggest 4%–20% of infected... Read more
Metabolic Biomarker Distinguishes Latent from Active Tuberculosis and Tracks Treatment Response
Tuberculosis (TB) remains the world’s leading infectious killer, with 10.8 million cases and 1.25 million deaths recorded globally in 2023. Yet many infected individuals never develop active disease, underscoring... Read more
Microbiology
view channel
New Culture Medium Speeds C. difficile Resistance Detection and Reduces Costs
Clostridioides difficile infections remain a persistent threat in hospitals and communities, affecting about 500,000 people in the United States each year. Severe cases can be fatal within 30 days of diagnosis,... Read more
Automated Blood Culture System Speeds Detection of Bloodstream Infections
Bloodstream infections and sepsis require rapid laboratory detection to guide targeted antimicrobial therapy and reduce mortality. Conventional blood culture workflows can delay actionable results by critical... Read more
Pathology
view channel
3D Spatial Multi-Omics Maps Intra-Tumor Diversity in Colorectal Cancer
Colorectal cancer remains a leading cause of cancer death, and clinical decision-making is complicated by marked intra-tumor heterogeneity. Conventional bulk sequencing averages molecular signals across... Read more
Blood-Based Method Tracks Gene Activity in the Living Brain
Real-time measurement of gene activity in the brain has been limited by assays requiring destructive tissue sampling. Tracking active genes could reveal how the body responds to environmental factors,... Read more
Technology
view channel
AI Platform Links Biomarker Results to Cancer Clinical Trials and Guidelines
Oncology teams must manage growing volumes of genomic data, rapidly evolving clinical trial options, and frequently updated care guidelines, all within tight clinic schedules. Translating complex tumor... Read more
Agentic AI Platform Supports Genomic Decision-Making in Oncology
Oncology care teams increasingly face the challenge of managing complex molecular diagnostics, evolving treatment options, and extensive electronic health record documentation. Translating multimodal data... Read more
Industry
view channel
Collaboration Advances ctDNA-Guided Development in Metastatic Colorectal Cancer
Natera, Inc. (Austin, TX, USA) and CytoDyn Inc. (Vancouver, WA, USA) announced a strategic collaboration focused on metastatic colorectal cancer (mCRC). Under the agreement, Natera will evaluate circulating... Read more
